{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T02:21:06Z","timestamp":1792462866393,"version":"4.5.0"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[1998,6]]},"abstract":"<jats:p><jats:italic>Abstract<\/jats:italic>\u2014The renal kallikrein-kinin system is activated under conditions of mineralocorticoid excess. To evaluate whether endogenous kinins exert a protective role against the development of mineralocorticoid-induced hypertension, we studied the cardiovascular effects induced by long-term administration of deoxycorticosterone (DOC; 0.3 \u03bcmol\/g body wt SC once per week for 6 weeks) or vehicle in transgenic mice (Bk2r<jats:sup>\u2212\/\u2212<\/jats:sup>) lacking the bradykinin B<jats:sub>2<\/jats:sub>receptor gene and in wild-type controls (Bk2r<jats:sup>+\/+<\/jats:sup>). Under basal conditions, Bk2r<jats:sup>\u2212\/\u2212<\/jats:sup>mice showed higher systolic blood pressure (tail-cuff plethysmography) than wild-type Bk2r<jats:sup>+\/+<\/jats:sup>and heterozygous Bk2r<jats:sup>+\/\u2212<\/jats:sup>mice (121\u00b12 versus 114\u00b12 and 115\u00b12 mm Hg, respectively;<jats:italic>P<\/jats:italic>&lt;0.05 for both comparisons). Heart rate was higher in Bk2r<jats:sup>\u2212\/\u2212<\/jats:sup>and Bk2r<jats:sup>+\/\u2212<\/jats:sup>than in Bk2r<jats:sup>+\/+<\/jats:sup>(459\u00b112 and 418\u00b17 versus 390\u00b17 bpm;<jats:italic>P<\/jats:italic>&lt;0.05 for both comparisons). Systolic blood pressure was increased by DOC in transgenic as well as in wild-type mice, whereas no change was induced by the vehicle. The pressor response to DOC was more rapid and pronounced in Bk2r<jats:sup>\u2212\/\u2212<\/jats:sup>than in Bk2r<jats:sup>+\/+<\/jats:sup>and Bk2r<jats:sup>+\/\u2212<\/jats:sup>(30\u00b15 versus 15\u00b14 and 6\u00b13 mm Hg, respectively, at 3 weeks;<jats:italic>P<\/jats:italic>&lt;0.01 for both comparisons). The difference in systolic blood pressure was consistent with that detected by direct intra-arterial measurements of mean blood pressure. Neither DOC nor its vehicle altered heart rate or gain in body weight over time. Under basal conditions, urinary sodium excretion did not differ between strains. During DOC administration, cumulative urinary sodium excretion was lower in Bk2r<jats:sup>\u2212\/\u2212<\/jats:sup>than in Bk2r<jats:sup>+\/+<\/jats:sup>(2.59\u00b10.15 versus 3.31\u00b10.22 mmol, respectively, during the first week;<jats:italic>P<\/jats:italic>&lt;0.05). Urinary kinin excretion was increased by DOC in both Bk2r<jats:sup>\u2212\/\u2212<\/jats:sup>(from 0.65\u00b10.17 to 4.27\u00b10.80 pmol\/24 h;<jats:italic>P<\/jats:italic>&lt;0.01) and Bk2r<jats:sup>+\/+<\/jats:sup>(from 0.55\u00b10.09 to 6.27\u00b11.48 pmol\/24 h;<jats:italic>P<\/jats:italic>&lt;0.05). The increase in urinary kinin excretion was similar between strains. These results show that integrity of the bradykinin B<jats:sub>2<\/jats:sub>receptor is essential for regulation of blood pressure and heart rate under basal conditions. In addition, they indicate that activation of the kallikrein-kinin system represents a compensatory response against the development of hypertension induced by mineralocorticoid excess.<\/jats:p>","DOI":"10.1161\/01.hyp.31.6.1278","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:53:46Z","timestamp":1339462426000},"page":"1278-1283","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":27,"title":["Enhanced Blood Pressure Sensitivity to Deoxycorticosterone in Mice With Disruption of Bradykinin B<sub>2<\/sub>Receptor Gene"],"prefix":"10.1161","volume":"31","author":[{"given":"Costanza","family":"Emanueli","sequence":"first","affiliation":[{"name":"From the Departments of Farmacologia (C.E., M.P.D.), Biochimica (A.F.M., M.B.S.), and Clinica Medica (M.C., P.M.), University of Sassari (Italy); Department of Clinical Biochemistry, University of Munich (Germany) (E.F.); and National Institute of Biostructures and Biosystems, Osilo, Italy (P.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin","family":"Fink","sequence":"additional","affiliation":[{"name":"From the Departments of Farmacologia (C.E., M.P.D.), Biochimica (A.F.M., M.B.S.), and Clinica Medica (M.C., P.M.), University of Sassari (Italy); Department of Clinical Biochemistry, University of Munich (Germany) (E.F.); and National Institute of Biostructures and Biosystems, Osilo, Italy (P.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Franca","family":"Milia","sequence":"additional","affiliation":[{"name":"From the Departments of Farmacologia (C.E., M.P.D.), Biochimica (A.F.M., M.B.S.), and Clinica Medica (M.C., P.M.), University of Sassari (Italy); Department of Clinical Biochemistry, University of Munich (Germany) (E.F.); and National Institute of Biostructures and Biosystems, Osilo, Italy (P.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Bonaria","family":"Salis","sequence":"additional","affiliation":[{"name":"From the Departments of Farmacologia (C.E., M.P.D.), Biochimica (A.F.M., M.B.S.), and Clinica Medica (M.C., P.M.), University of Sassari (Italy); Department of Clinical Biochemistry, University of Munich (Germany) (E.F.); and National Institute of Biostructures and Biosystems, Osilo, Italy (P.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena","family":"Conti","sequence":"additional","affiliation":[{"name":"From the Departments of Farmacologia (C.E., M.P.D.), Biochimica (A.F.M., M.B.S.), and Clinica Medica (M.C., P.M.), University of Sassari (Italy); Department of Clinical Biochemistry, University of Munich (Germany) (E.F.); and National Institute of Biostructures and Biosystems, Osilo, Italy (P.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Piera","family":"Demontis","sequence":"additional","affiliation":[{"name":"From the Departments of Farmacologia (C.E., M.P.D.), Biochimica (A.F.M., M.B.S.), and Clinica Medica (M.C., P.M.), University of Sassari (Italy); Department of Clinical Biochemistry, University of Munich (Germany) (E.F.); and National Institute of Biostructures and Biosystems, Osilo, Italy (P.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Madeddu","sequence":"additional","affiliation":[{"name":"From the Departments of Farmacologia (C.E., M.P.D.), Biochimica (A.F.M., M.B.S.), and Clinica Medica (M.C., P.M.), University of Sassari (Italy); Department of Clinical Biochemistry, University of Munich (Germany) (E.F.); and National Institute of Biostructures and Biosystems, Osilo, Italy (P.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.13.6.658"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1969.217.1.1"},{"key":"e_1_3_2_3_2","first-page":"F87","volume":"248","year":"1985","unstructured":"Granger JP, Hall JE. 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