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The systolic blood pressure was measured by the tail-cuff method twice a week. Food and water intake and urine flow were measured daily. The results showed that sustained insulin infusion significantly increased plasma insulin concentrations from 277.7\u00b125.8 pmol\/L to 609.9\u00b122.2 and 696.7\u00b123.0 pmol\/L by the end of weeks 4 and 6, respectively (<jats:italic>P<\/jats:italic>&lt;0.05). Systolic blood pressure was significantly increased from 135\u00b13 to 157\u00b13 and 159\u00b12 mm Hg (<jats:italic>P<\/jats:italic>&lt;0.05) at the corresponding time points. There was a significant increase in the plasma norepinephrine concentration after insulin infusion, whereas no significant changes in plasma triglyceride and glucose concentrations, water intake, urine flow, sodium excretion, sodium gain, and body weight gain were observed. Bilateral renal denervation depleted renal norepinephrine stores and prevented the development of hyperinsulinemia-induced hypertension. After hyperinsulinemia-induced hypertension had been fully established (from 134\u00b12 to 157\u00b12 mm Hg), bilateral renal denervation reversed the elevated systolic blood pressure to normotensive levels within 2 weeks. Transient denervated diuresis and natriuresis were observed. These results indicate that chronic hyperinsulinemia-induced hypertension requires the presence of intact renal nerves in rats.<\/jats:p>","DOI":"10.1161\/01.hyp.32.2.249","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:53:46Z","timestamp":1339462426000},"page":"249-254","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":44,"title":["Renal Denervation Prevents and Reverses Hyperinsulinemia-Induced Hypertension in Rats"],"prefix":"10.1161","volume":"32","author":[{"given":"Wann-Chu","family":"Huang","sequence":"first","affiliation":[{"name":"From the Graduate Institute of Medical Sciences, Tzu Chi College of Medicine (W.-C.H.), Division of Nephrology; the Department of Medicine, Tzu Chi General Hospital (T.-C.F.); and the Department of Pharmacology, College of Medicine, National Cheng Kung University (J.-T.C.), Taiwan, Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Te-Chao","family":"Fang","sequence":"additional","affiliation":[{"name":"From the Graduate Institute of Medical Sciences, Tzu Chi College of Medicine (W.-C.H.), Division of Nephrology; the Department of Medicine, Tzu Chi General Hospital (T.-C.F.); and the Department of Pharmacology, College of Medicine, National Cheng Kung University (J.-T.C.), Taiwan, Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juei-Tang","family":"Cheng","sequence":"additional","affiliation":[{"name":"From the Graduate Institute of Medical Sciences, Tzu Chi College of Medicine (W.-C.H.), Division of Nephrology; the Department of Medicine, Tzu Chi General Hospital (T.-C.F.); and the Department of Pharmacology, College of Medicine, National Cheng Kung University (J.-T.C.), Taiwan, Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198708063170605"},{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","unstructured":"Weidmann P Bohlen L de Courten M. 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