{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T11:29:15Z","timestamp":1765279755477},"reference-count":24,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:p>\n            <jats:italic>Abstract<\/jats:italic>\n            \u2014It is known that hyperaldosteronism has been associated with magnesium deficiency, yet there are no data on the intracellular concentration of ionized magnesium ([Mg\n            <jats:sup>2+<\/jats:sup>\n            <jats:sub>i<\/jats:sub>\n            ]) in subjects with primary aldosteronism (PA). We measured intralymphocyte free magnesium ([Mg\n            <jats:sup>2+<\/jats:sup>\n            <jats:sub>i<\/jats:sub>\n            ]) and intralymphocyte free calcium ([Ca\n            <jats:sup>2+<\/jats:sup>\n            <jats:sub>i<\/jats:sub>\n            ]) in 16 patients with PA and 26 normotensive control subjects (NCs). [Mg\n            <jats:sup>2+<\/jats:sup>\n            <jats:sub>i<\/jats:sub>\n            ] and [Ca\n            <jats:sup>2+<\/jats:sup>\n            <jats:sub>i<\/jats:sub>\n            ] were also measured in blood lymphocytes incubated in vitro with aldosterone, according to a fluorimetric method. In subjects with PA, [Mg\n            <jats:sup>2+<\/jats:sup>\n            <jats:sub>i<\/jats:sub>\n            ] was significantly lower than that in NCs (mean\u00b1SD; PA 203\u00b156 \u03bcmol\/L, NCs 291\u00b143 \u03bcmol\/L, 95% confidence interval 57 to 119,\n            <jats:italic>P<\/jats:italic>\n            =0.001). In the patients, [Ca\n            <jats:sup>2+<\/jats:sup>\n            <jats:sub>i<\/jats:sub>\n            ] did not prove to be statistically different from that of NCs (mean\u00b1SD; PA 47.2\u00b110.6 nmol\/L, NCs 53.2\u00b111 nmol\/L). The lymphocytes exposed to the action of aldosterone showed a significant reduction in [Mg\n            <jats:sup>2+<\/jats:sup>\n            <jats:sub>i<\/jats:sub>\n            ] (n=15, NCs 271\u00b128 \u03bcmol\/L, aldosterone treatment 188\u00b139 \u03bcmol\/L,\n            <jats:italic>P<\/jats:italic>\n            =0.001, 95% confidence interval 57 to 108). The dose-effect curve of aldosterone on [Mg\n            <jats:sup>2+<\/jats:sup>\n            <jats:sub>i<\/jats:sub>\n            ] showed an EC\n            <jats:sub>50<\/jats:sub>\n            value of \u22480.5 to 1 nmol\/L aldosterone. The reduction in [Mg\n            <jats:sup>2+<\/jats:sup>\n            <jats:sub>i<\/jats:sub>\n            ] mediated by aldosterone is antagonized by the receptor inhibitor of aldosterone; it is inhibited by inhibitors of protein synthesis and is not measurable when the lymphocytes are incubated in an Na\n            <jats:sup>+<\/jats:sup>\n            -free medium. The data are consistent with the hypothesis that aldosterone affects the cellular homeostasis of magnesium, probably through modification of the activity of the Na\n            <jats:sup>+<\/jats:sup>\n            -Mg\n            <jats:sup>2+<\/jats:sup>\n            antiporter.\n          <\/jats:p>","DOI":"10.1161\/01.hyp.35.1.113","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:25:31Z","timestamp":1339460731000},"page":"113-117","update-policy":"http:\/\/dx.doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":32,"title":["Intralymphocyte Free Magnesium in Patients With Primary Aldosteronism"],"prefix":"10.1161","volume":"35","author":[{"given":"Pietro","family":"Delva","sequence":"first","affiliation":[{"name":"From the Departments of Biomedic and Surgical Sciences (P.D., C.P., M.D., G.M., A.L.) and Surgery (P.B.), University of Verona, Policlinico Borgo Roma, Verona, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caterina","family":"Pastori","sequence":"additional","affiliation":[{"name":"From the Departments of Biomedic and Surgical Sciences (P.D., C.P., M.D., G.M., A.L.) and Surgery (P.B.), University of Verona, Policlinico Borgo Roma, Verona, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurizio","family":"Degan","sequence":"additional","affiliation":[{"name":"From the Departments of Biomedic and Surgical Sciences (P.D., C.P., M.D., G.M., A.L.) and Surgery (P.B.), University of Verona, Policlinico Borgo Roma, Verona, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Germana","family":"Montesi","sequence":"additional","affiliation":[{"name":"From the Departments of Biomedic and Surgical Sciences (P.D., C.P., M.D., G.M., A.L.) and Surgery (P.B.), University of Verona, Policlinico Borgo Roma, Verona, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Brazzarola","sequence":"additional","affiliation":[{"name":"From the Departments of Biomedic and Surgical Sciences (P.D., C.P., M.D., G.M., A.L.) and Surgery (P.B.), University of Verona, Policlinico Borgo Roma, Verona, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Lechi","sequence":"additional","affiliation":[{"name":"From the Departments of Biomedic and Surgical Sciences (P.D., C.P., M.D., G.M., A.L.) and Surgery (P.B.), University of Verona, Policlinico Borgo Roma, Verona, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.1994.tb02021.x"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/hyp.29.2.587"},{"key":"e_1_3_2_3_2","unstructured":"Delva P Fallo F Mantero F Capra C Degan M Minuz P Lechi A. 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