{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T13:33:58Z","timestamp":1780666438962,"version":"3.54.1"},"reference-count":45,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[2000,8]]},"abstract":"<jats:p>\n            <jats:italic>Abstract<\/jats:italic>\n            \u2014Ascorbic acid and  glutathione (GSH) are important determinants of the intracellular redox  state, and both are known to accelerate the decomposition of\n            <jats:italic>S<\/jats:italic>\n            -nitrosoglutathione (GSNO), an endogenous  adduct of nitric oxide (NO). The implications of these observations for  GSNO bioactivity are not yet clear. We investigated the effect of  ascorbic acid and GSH on GSNO bioactivity by using a bioassay with  isolated segments of guinea pig aorta suspended in organ chambers.  Arterial segments demonstrated relaxation to GSNO (0.1  \u03bcmol\/L) that was significantly enhanced by 300 \u03bcmol\/L ascorbic  acid (71\u00b16% versus 53\u00b16%,\n            <jats:italic>P<\/jats:italic>\n            &lt;0.05) but not GSH. Both  ascorbic acid and GSH significantly shortened the duration of  arterial relaxation in response to 0.1 \u03bcmol\/L GSNO  (from &gt;120 minutes to 22.5\u00b13.5 and 36.3\u00b14.3 minutes, respectively;\n            <jats:italic>P<\/jats:italic>\n            &lt;0.05), consistent with accelerated decomposition  of GSNO that was confirmed spectrophotometrically. The effect of  ascorbic acid was abrogated by either DTPA or the copper(I)-specific  agent bathocuproine but not deferoxamine, indicating  a dependence on the availability of redox-active copper.  Consistent with this notion, the action of ascorbic acid on  GSNO bioactivity was also supported by copper-zinc superoxide  dismutase, a physiologically relevant source of  copper. In contrast, the effect of GSH on GSNO degradation and  GSNO-mediated arterial relaxation was independent of  transition metal ions, because DTPA had no effect. These data indicate  that both ascorbic acid and GSH modulate GSNO bioactivity and suggest a  distinction between the mechanism of GSNO degradation by ascorbic acid  or GSH. Whereas both ascorbic acid and GSH accelerate the degradation  of GSNO, only ascorbic acid is dependent on the presence of transition  metal ions.\n          <\/jats:p>","DOI":"10.1161\/01.hyp.36.2.291","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:25:31Z","timestamp":1339460731000},"page":"291-295","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":37,"title":["Ascorbic Acid and Glutathione Modulate the Biological Activity of\n            <i>S-<\/i>\n            Nitrosoglutathione"],"prefix":"10.1161","volume":"36","author":[{"given":"Aiming","family":"Xu","sequence":"first","affiliation":[{"name":"From the Evans Memorial Department of Medicine and Whitaker  Cardiovascular Institute, Boston University School of Medicine, Boston, Mass."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph A.","family":"Vita","sequence":"additional","affiliation":[{"name":"From the Evans Memorial Department of Medicine and Whitaker  Cardiovascular Institute, Boston University School of Medicine, Boston, Mass."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"John F.","family":"Keaney","sequence":"additional","affiliation":[{"name":"From the Evans Memorial Department of Medicine and Whitaker  Cardiovascular Institute, Boston University School of Medicine, Boston, Mass."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.14.6348"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117852"},{"key":"e_1_3_2_3_2","first-page":"262","volume":"9","year":"1996","unstructured":"Welch GN, Upchurch GR Jr, Loscalzo J. Nitric oxide as a vascular modulator. Blood Rev. 1996;9:262\u2013269.","journal-title":"Blood Rev"},{"key":"e_1_3_2_4_2","first-page":"H931","volume":"267","year":"1994","unstructured":"Kubes P, Kurose I, Granger DN. NO donors prevent integrin-induced leukocyte adhesion but not P-selectin-dependent rolling in postischemic venules. Am J Physiol. 1994;267:H931\u2013H937.","journal-title":"Am J Physiol"},{"key":"e_1_3_2_5_2","first-page":"2630","volume":"95","year":"1995","unstructured":"Marks DS, Vita JA, Folts JD, Keaney JF Jr, Welch GN, Loscalzo J. Inhibition of neointimal proliferation in rabbits after vascular injury by a single treatment with a protein adduct of nitric oxide. J Clin Invest. 1995;95:2630\u20132638.","journal-title":"J Clin Invest"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114081"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/377239a0"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.23.13176"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0033-0620(05)80003-9"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/tx00040a007"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.5.2841"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116364"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1281928"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1139\/y93-111"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.47.28158"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.31.18596"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.9.3680"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/381380a0"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1996.0247"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(96)68032-X"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)00560-1"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.25.14492"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)00086-5"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117480"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1039\/an9588300670"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1161\/atvb.17.11.2975"},{"key":"e_1_3_2_27_2","first-page":"H924","volume":"271","year":"1996","unstructured":"Jackson TS, Lerner E, Weisbrod RM, Tajima M, Loscalzo J, Keaney JF Jr. The vasodilatory properties of recombinant maxadilan. Am J Physiol. 1996;271:H924\u2013H930.","journal-title":"Am J Physiol"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.3109\/10715769009145927"},{"key":"e_1_3_2_29_2","first-page":"1758","volume":"93","year":"1993","unstructured":"McAninly J, Williams DLH, Askew SC, Butler AR, Russel C. Metal ion catalysis in nitrosothiol (RSNO) decomposition. J Chem Soc Chem Commun. 1993;93:1758\u20131759.","journal-title":"J Chem Soc Chem Commun"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(95)00089-U"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.10.5158"},{"key":"e_1_3_2_32_2","unstructured":"Stocker R Frei B. Endogenous antioxidant defences in human blood plasma. In: Sies H ed. Oxidative Stress: Oxidants and Antioxidants. London England: Academic Press; 1991:213\u2013243."},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a021883"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)83589-X"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1993.1327"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(92)90507-S"},{"key":"e_1_3_2_37_2","first-page":"142","volume":"317","year":"1994","unstructured":"Spear N, Aust SD. Hydroxylation of deoxyguanosine in DNA by copper and thiols. Arch Biochem Biophys. 1994;317:142\u2013148.","journal-title":"Arch Biochem Biophys"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.3.1044"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.29.17355"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)74235-5"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5414.651"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5321.2034"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1998.1010"},{"key":"e_1_3_2_44_2","first-page":"1107","volume":"96","year":"1996","unstructured":"Levine GN, Frei B, Koulouris SN, Gerhard MD, Keaney JF Jr, Vita JA. Ascorbic acid reverses endothelial dysfunction in patients with coronary artery disease. Circulation. 1996;96:1107\u20131113.","journal-title":"Circulation"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)04410-4"}],"container-title":["Hypertension"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.HYP.36.2.291","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T17:39:17Z","timestamp":1715535557000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.HYP.36.2.291"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,8]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2000,8]]}},"alternative-id":["10.1161\/01.HYP.36.2.291"],"URL":"https:\/\/doi.org\/10.1161\/01.hyp.36.2.291","relation":{},"ISSN":["0194-911X","1524-4563"],"issn-type":[{"value":"0194-911X","type":"print"},{"value":"1524-4563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,8]]},"assertion":[{"value":"1999-10-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-02-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}