{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:39:33Z","timestamp":1791092373939,"version":"4.1.0"},"reference-count":32,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[2000,10]]},"abstract":"<jats:p>\n            <jats:italic>Abstract<\/jats:italic>\n            \u2014Aldosterone  is synthesized in extra-adrenal tissues, both blood vessels and brain.  We undertook the present study to determine whether the rat heart  produces aldosterone and to investigate the effects of  adrenalectomy, ACE inhibition, and angiotensin II on  aldosterone synthesis in the heart. To clarify the  pathophysiological role of cardiac  aldosterone in the hypertensive heart, we compared the  synthesis of aldosterone in the hearts of stroke-prone  spontaneously hypertensive rats (SHRSP) with that in Wistar-Kyoto rats.  The effects of the aldosterone antagonist  spironolactone on myocardial hypertrophy in  adrenalectomized SHRSP were also studied. Isolated rat hearts were  perfused for 2 hours, and the perfusate was analyzed  with HPLC and mass spectrometry. The activity of  aldosterone synthase was estimated on the basis of the  conversion of [\n            <jats:sup>14<\/jats:sup>\n            C]deoxycorticosterone to  [\n            <jats:sup>14<\/jats:sup>\n            C]aldosterone. The levels of  aldosterone synthase gene (CYP11B2) mRNA were determined  with competitive polymerase chain reaction. Aldosterone  production, the activity of aldosterone synthase,  and the expression of CYP11B2 mRNA were increased in hearts from  adrenalectomized rats and rats treated with angiotensin II.  ACE inhibitors decreased cardiac aldosterone  synthesis. Cardiac aldosterone, aldosterone  synthase activity, and CYP11B2 mRNA levels in hearts from 2- and  4-week-old SHRSP were significantly greater than those of age-matched  Wistar-Kyoto rats. Spironolactone prevented cardiac  hypertrophy in adrenalectomized SHRSP. These results  suggest that the rat heart produces aldosterone and that  endogenous cardiac aldosterone may affect  cardiac function and hypertrophy in hypertension in  rats.\n          <\/jats:p>","DOI":"10.1161\/01.hyp.36.4.495","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:25:31Z","timestamp":1339460731000},"page":"495-500","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":105,"title":["Cardiac Aldosterone Production in Genetically Hypertensive Rats"],"prefix":"10.1161","volume":"36","author":[{"given":"Yoshiyu","family":"Takeda","sequence":"first","affiliation":[{"name":"From the Second Department of Internal Medicine (Y.T., T.Y., M.D., H.M.),  Department of Health Sciences (Y.T.), School of Medicine, Kanazawa University,  Kanazawa, Japan; and Third Department of Internal Medicine (I.M.), Fukui  Medical School, Fukui, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Yoneda","sequence":"additional","affiliation":[{"name":"From the Second Department of Internal Medicine (Y.T., T.Y., M.D., H.M.),  Department of Health Sciences (Y.T.), School of Medicine, Kanazawa University,  Kanazawa, Japan; and Third Department of Internal Medicine (I.M.), Fukui  Medical School, Fukui, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Demura","sequence":"additional","affiliation":[{"name":"From the Second Department of Internal Medicine (Y.T., T.Y., M.D., H.M.),  Department of Health Sciences (Y.T.), School of Medicine, Kanazawa University,  Kanazawa, Japan; and Third Department of Internal Medicine (I.M.), Fukui  Medical School, Fukui, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isamu","family":"Miyamori","sequence":"additional","affiliation":[{"name":"From the Second Department of Internal Medicine (Y.T., T.Y., M.D., H.M.),  Department of Health Sciences (Y.T.), School of Medicine, Kanazawa University,  Kanazawa, Japan; and Third Department of Internal Medicine (I.M.), Fukui  Medical School, Fukui, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Mabuchi","sequence":"additional","affiliation":[{"name":"From the Second Department of Internal Medicine (Y.T., T.Y., M.D., H.M.),  Department of Health Sciences (Y.T.), School of Medicine, Kanazawa University,  Kanazawa, Japan; and Third Department of Internal Medicine (I.M.), Fukui  Medical School, Fukui, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117269"},{"key":"e_1_3_2_2_2","first-page":"E657","volume":"269","year":"1995","unstructured":"Young M, Head G, Funder JW. 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