{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,17]],"date-time":"2024-05-17T23:23:39Z","timestamp":1715988219498},"reference-count":18,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[2000,12]]},"abstract":"<jats:p>\n            <jats:italic>Abstract<\/jats:italic>\n            \u2014A sodium pump  inhibitor (digitalis-like factor), isolated from the  peritoneal dialysate of volume-expanded, hypertensive patients with  kidney failure who were treated with this dialysis modality, was  further purified and characterized by means of supercritical fluid  chromatography, a separation technique whose  application to very-low-concentration biomolecules is new. Previous  studies suggested that after high-performance liquid  chromatography (HPLC) purification, this  inhibitor was the only factor correlated with volume status  and blood pressure in these patients. When this same HPLC fraction was  furthered purified on 2-dimensional supercritical fluid  chromatography, a single peak coeluted with  [Na,K]ATPase inhibitory activity. When split specimens  were used, there was a strict correlation between the peak area,  measured by flame ionization detection, and activity (n=10,\n            <jats:italic>R<\/jats:italic>\n            =0.98,\n            <jats:italic>P<\/jats:italic>\n            =0.00001).  Inhibitory activity after supercritical fluid  chromatography was still correlated with the degree of  volume expansion of donor patients (\n            <jats:italic>P<\/jats:italic>\n            =0.01). After HPLC  purification, this volume-sensitive inhibitor was  chemically labile. With further purification on supercritical fluid  chromatography, the active peak was still labile with  comparable half-life. Supercritical fluid  chromatography coupled with flame ionization detection  provided an estimate of the amount of the inhibitor  present. Again using split specimens, we determined that the labile  digitalis-like factor was \u224830-fold more effective than ouabain in  inhibiting renal [Na,K]ATPase activity and \u2265500 times more effective  than ouabain in causing vascular smooth muscle contraction. The data  suggest that we have purified to homogeneity a labile digitalis-like  factor that is readily distinguished from ouabain or bufalin, based on  chromatographic characteristics, chemical lability, and a  much lower effective concentration for its biological  activity.\n          <\/jats:p>","DOI":"10.1161\/01.hyp.36.6.1059","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:25:31Z","timestamp":1339460731000},"page":"1059-1064","update-policy":"http:\/\/dx.doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":9,"title":["Application of Supercritical Fluid Chromatography to Characterize a Labile Digitalis-Like Factor"],"prefix":"10.1161","volume":"36","author":[{"given":"Steven W.","family":"Graves","sequence":"first","affiliation":[{"name":"From the Department of Chemistry and Biochemistry, Brigham Young  University, Provo, Utah (S.W.G.); the Department of Analytical Chemistry,  Uppsala University, Uppsala, Sweden (K.E.M.); and the Department of Radiology,  Brigham and Women\u2019s Hospital, Harvard Medical School, Boston, Mass  (N.K.H.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen E.","family":"Markides","sequence":"additional","affiliation":[{"name":"From the Department of Chemistry and Biochemistry, Brigham Young  University, Provo, Utah (S.W.G.); the Department of Analytical Chemistry,  Uppsala University, Uppsala, Sweden (K.E.M.); and the Department of Radiology,  Brigham and Women\u2019s Hospital, Harvard Medical School, Boston, Mass  (N.K.H.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norman K.","family":"Hollenberg","sequence":"additional","affiliation":[{"name":"From the Department of Chemistry and Biochemistry, Brigham Young  University, Provo, Utah (S.W.G.); the Department of Analytical Chemistry,  Uppsala University, Uppsala, Sweden (K.E.M.); and the Department of Radiology,  Brigham and Women\u2019s Hospital, Harvard Medical School, Boston, Mass  (N.K.H.)."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/258577a0"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(76)90002-3"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1974.2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.4.5.581"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","unstructured":"Pamnani MB Huot SJ Buggy J Clough DL Haddy FJ. Demonstration of a humoral inhibitor of the Na+-K+ pump in some models of experimental hypertension. Hypertension. 1981;3(S2):96\u2013101.","DOI":"10.1161\/01.HYP.3.6_Pt_2.II-96"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajh\/7.11.1016"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.282.6267.847"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/300650a0"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(85)90562-4"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","unstructured":"Hollenberg NK Graves SW. Endogenous sodium pump inhibition: current status and therapeutic opportunities. In: Jucker E ed. Progress in Drug Research. Basel Switzerland: Birkhauser Verlag; 1996:9\u201342.","DOI":"10.1007\/978-3-0348-8996-4_1"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.14.6259"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/hyp.31.5.1097"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1996.61"},{"key":"e_1_3_2_14_2","first-page":"815","volume":"29","year":"1996","unstructured":"Tao Q-F, Hollenberg NK, Price DA, Graves SW. Sodium pump isoform specificity for the digitalis-like factor isolated from human peritoneal dialysate. Hypertension. 1996;29:815\u2013821.","journal-title":"Hypertension"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02278586"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-60-6-1135"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199506000-00002"},{"key":"e_1_3_2_18_2","first-page":"39","volume":"9","year":"1995","unstructured":"Krep HH, Graves SW, Price DA, Lazarus M, Ensign A, Soszynski PA, Hollenberg NK. Reversal of sodium pump inhibitor induced vascular smooth muscle contraction with Digibind: stoichiometry and its implication. Am J Hypertens. 1995;9:39\u201346.","journal-title":"Am J Hypertens"}],"container-title":["Hypertension"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.HYP.36.6.1059","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T17:49:48Z","timestamp":1715536188000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.HYP.36.6.1059"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,12]]},"references-count":18,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2000,12]]}},"alternative-id":["10.1161\/01.HYP.36.6.1059"],"URL":"https:\/\/doi.org\/10.1161\/01.hyp.36.6.1059","relation":{},"ISSN":["0194-911X","1524-4563"],"issn-type":[{"value":"0194-911X","type":"print"},{"value":"1524-4563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,12]]},"assertion":[{"value":"2000-03-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-06-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}