{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:51:02Z","timestamp":1790693462286,"version":"4.1.0"},"reference-count":33,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[2001,2]]},"abstract":"<jats:p>\n            Growth  arrest and DNA damage inducible gene 153 (\n            <jats:italic>gadd153<\/jats:italic>\n            ) is  expressed at very low levels in growing cells but is markedly induced  in response to cellular stresses, including glucose  deprivation, exposure to genotoxic agents, and other  growth-arresting situations. Forced expression of GADD153 can  induce cell cycle arrest and\/or apoptosis in many types of  cells. Recently, we reported that GADD153 was induced in vascular  smooth muscle cells (VSMCs) in neointimal lesions of  balloon-injured carotid arteries. To investigate the underlying  molecular mechanisms of\n            <jats:italic>gadd153<\/jats:italic>\n            gene expression in  VSMCs, we isolated and characterized a promoter region of the rat\n            <jats:italic>gadd153<\/jats:italic>\n            gene. Sequence alignments of this region  revealed 1 TATA-like sequence and several well-known\n            <jats:italic>cis<\/jats:italic>\n            elements. The 5\u2032-deletion  analysis for this region showed that a domain spanning \u2212447  through \u2212368 drastically reduced the promoter activity to almost  equal levels of promoterless control. Because this domain contained a  consensus sequence for the nuclear factor 1 family of proteins (NF1),  DNA-binding studies were performed by use of 2 types of NF1 consensus  probes. Both probes were specifically shifted by nuclear extracts from  proliferating VSMCs and were supershifted by antiserum against CCAAT  transcription factor\/NF1. In addition, promoter activity of a mutant  luciferase vector, which was generated by a point mutation at the NF1  binding motif of the gadd153 gene, was 14-fold higher than that of a  wild-type one. These results suggest that\n            <jats:italic>gadd153<\/jats:italic>\n            gene  expression in VSMCs is negatively regulated by an NF1-binding motif,  and NF1 may act as an antiapoptotic factor by continuously  suppressing\n            <jats:italic>gadd153<\/jats:italic>\n            gene expression in growing  VSMCs.\n          <\/jats:p>","DOI":"10.1161\/01.hyp.37.2.419","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:25:31Z","timestamp":1339460731000},"page":"419-424","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":13,"title":["Nuclear Factor 1 Is a Negative Regulator of\n            <i>gadd153<\/i>\n            Gene Expression in Vascular Smooth Muscle Cells"],"prefix":"10.1161","volume":"37","author":[{"given":"Michitsugu","family":"Nakamura","sequence":"first","affiliation":[{"name":"From The Second Department of Internal Medicine, Ehime University School  of Medicine, Ehime, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takafumi","family":"Okura","sequence":"additional","affiliation":[{"name":"From The Second Department of Internal Medicine, Ehime University School  of Medicine, Ehime, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Kitami","sequence":"additional","affiliation":[{"name":"From The Second Department of Internal Medicine, Ehime University School  of Medicine, Ehime, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunio","family":"Hiwada","sequence":"additional","affiliation":[{"name":"From The Second Department of Internal Medicine, Ehime University School  of Medicine, Ehime, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.5.9.1538"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.13.8.4736"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.2337\/diab.42.5.738"},{"key":"e_1_3_2_4_2","first-page":"3814","volume":"52","year":"1992","unstructured":"Price BD, Calderwood SK. Gadd45 and gadd153 messenger RNA levels are increased during hypoxia and after exposure of cells to agents which elevate the levels of the glucose-regulated proteins. Cancer Res. 1992;52:3814\u20133817.","journal-title":"Cancer Res"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.4.453"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.4.2361"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.4.2352"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.8.2810"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113671"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/362801a0"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199201233260406"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/hyp.29.1.340"},{"key":"e_1_3_2_13_2","unstructured":"Igase M Okura T Nakamura M Takata Y Kitami Y Hiwada K. The role of GADD153 in vascular smooth muscle cell apoptosis. Clin Sci. In press."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(91)91328-A"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.36.25576"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/hyp.33.1.298"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)46254-5"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(92)90523-R"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3140547"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(00)00140-2"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.18.12917"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1994.tb18560.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.12.6975"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(99)00020-7"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.11.8.4065"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.23.8800"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.9.10.4196"},{"key":"e_1_3_2_28_2","first-page":"245","volume":"39","year":"1997","unstructured":"Delmastro DA, Li J, Vaisman A, Solle M, Chaney SG. DNA damage inducible-gene expression following platinum treatment in human ovarian carcinoma cell lines. Cancer Chemother Pharmacol. 1997;39:245\u2013253.","journal-title":"Cancer Chemother Pharmacol"},{"key":"e_1_3_2_29_2","first-page":"686","volume":"57","year":"1997","unstructured":"Eymin B, Dubrez L, Allouche M, Solary E. Increased gadd153 messenger RNA level is associated with apoptosis in human leukemic cells treated with etoposide. 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