{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:29:33Z","timestamp":1758126573283,"version":"3.40.3"},"reference-count":29,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[2001,2]]},"abstract":"<jats:p>Stimulation of \u03b2-adrenoceptors leads to vascular smooth muscle hyperpolarization, presumably through the \u03b2-adrenoceptors\/Gs protein\/adenylate cyclase\/ATP-sensitive K<jats:sup>+<\/jats:sup>-channels (KATP) signaling cascade, which may play an important role in the sympathetic control of membrane potential. \u03b2-Adrenoceptor\u2013mediated hyperpolarization has been shown to be impaired in the established stage of experimental hypertension. The present study tested the hypothesis that \u03b2-adrenergic hyperpolarization may be defective before the development of hypertension in some forms of genetic hypertension. We evaluated \u03b2-adrenoceptor\u2013mediated hyperpolarization using microelectrodes in mesenteric resistance arteries from 5-week-old, prehypertensive, spontaneously hypertensive rats (SHR) and age-matched Wistar-Kyoto rats (WKY). Isoproterenol-induced hyperpolarization was significantly smaller in SHR than in WKY (10<jats:sup>\u22127<\/jats:sup>mol\/L: \u22124.6\u00b10.6 versus \u22127.8\u00b10.8 mV,<jats:italic>P<\/jats:italic>&lt;0.01; 10<jats:sup>\u22126<\/jats:sup>mol\/L: \u22127.8\u00b10.5 versus \u22129.8\u00b10.6 mV,<jats:italic>P<\/jats:italic>&lt;0.05; n=9). Furthermore, hyperpolarization to cholera toxin, a direct activator of Gs protein, was also impaired in SHR. On the other hand, hyperpolarization to forskolin, an adenylate cyclase activator, and to levcromakalim, a KATP opener, was comparable between groups. These findings suggest that \u03b2-adrenoceptor\u2013mediated hyperpolarization is defective in SHR before the development of hypertension, presumably because of an abnormality at the Gs protein site. Considering the importance of membrane potential in the control of vascular tone, altered \u03b2-adrenergic control of membrane potential might play a role in the development of hypertension in SHR.<\/jats:p>","DOI":"10.1161\/01.hyp.37.2.609","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:25:31Z","timestamp":1339460731000},"page":"609-613","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":17,"title":["Impaired \u03b2-Adrenergic Hyperpolarization in Arteries From Prehypertensive Spontaneously Hypertensive Rats"],"prefix":"10.1161","volume":"37","author":[{"given":"Kenichi","family":"Goto","sequence":"first","affiliation":[{"name":"From the Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Fujii","sequence":"additional","affiliation":[{"name":"From the Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isao","family":"Abe","sequence":"additional","affiliation":[{"name":"From the Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.21.6.1005"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/hyp.34.2.222"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/S0022-3565(25)24288-4","volume":"272","year":"1995","unstructured":"Nakashima M, Vanhoutte PM. Isoproterenol causes hyperpolarization through opening of ATP-sensitive potassium channels in vascular smooth muscle of the canine saphenous vein. J Pharmacol Exp Ther. 1995;272:379\u2013384.","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","unstructured":"Goto K Fujii K Abe I Fujishima M. Sympathetic control of arterial membrane potential by ATP-sensitive K+ channels. 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