{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T13:54:14Z","timestamp":1760622854826,"version":"3.40.3"},"reference-count":31,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[2001,8]]},"abstract":"<jats:p>Transforming growth factor-\u03b2 (TGF-\u03b2) has been implicated in the development of interstitial fibrosis in cardiac hypertrophy. NO has been regarded as a potent inhibitor of cardiac fibroblast growth, albeit the modulation of cellular events associated with interstitial fibrosis remains undefined. In this regard, the regulation of TGF-\u03b2 mRNA expression by the NO donor<jats:italic>S<\/jats:italic>-nitroso-<jats:italic>N<\/jats:italic>-acetyl-penicillamine (SNAP) was examined in neonatal rat cardiac fibroblasts. SNAP treatment for 4 hours decreased TGF-\u03b2<jats:sub>3<\/jats:sub>mRNA levels, an effect mimicked by 8-bromo-cGMP. TGF-\u03b2<jats:sub>3<\/jats:sub>mRNA, however, had returned to levels observed in the untreated cells after a 24-hour exposure to SNAP, whereas a decreased expression persisted with 8-bromo-cGMP. In contrast to TGF-\u03b2<jats:sub>3<\/jats:sub>, TGF-\u03b2<jats:sub>1<\/jats:sub>mRNA levels were modestly increased in response to cGMP-generating molecules. The treatment with actinomycin D for at least 8 hours did not appreciably alter TGF-\u03b2<jats:sub>3<\/jats:sub>mRNA levels. By contrast, SNAP treatment caused a rapid decrease of TGF-\u03b2<jats:sub>3<\/jats:sub>mRNA with a half-life of 3.3\u00b10.2 hours, thereby supporting a mechanism of destabilization. The pretreatment with SNAP inhibited angiotensin II-stimulated protein synthesis and the concomitant expression of TGF-\u03b2<jats:sub>3<\/jats:sub>mRNA. These data reveal a disparate pattern of TGF-\u03b2<jats:sub>1<\/jats:sub>and TGF-\u03b2<jats:sub>3<\/jats:sub>mRNA regulation by NO and highlight a novel mechanism of destabilization contributing to the decreased expression of TGF-\u03b2<jats:sub>3<\/jats:sub>mRNA. The modulation of both basal and angiotensin II-stimulated TGF-\u03b2<jats:sub>3<\/jats:sub>mRNA expression provides a mechanism by which NO may influence the progression of interstitial fibrosis.<\/jats:p>","DOI":"10.1161\/01.hyp.38.2.261","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:25:31Z","timestamp":1339460731000},"page":"261-266","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":24,"title":["Nitric Oxide Attenuates the Expression of Transforming Growth Factor-\u03b2<sub>3<\/sub>mRNA in Rat Cardiac Fibroblasts via Destabilization"],"prefix":"10.1161","volume":"38","author":[{"given":"Nadia","family":"Abdelaziz","sequence":"first","affiliation":[{"name":"From the Universite\u0301 de Montre\u0301al, Departeme\u0301nt de Physiologie, et l\u2019Institut de Cardiologie de Montre\u0301al, Montre\u0301al, Que\u0301bec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Colombo","sequence":"additional","affiliation":[{"name":"From the Universite\u0301 de Montre\u0301al, Departeme\u0301nt de Physiologie, et l\u2019Institut de Cardiologie de Montre\u0301al, Montre\u0301al, Que\u0301bec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Mercier","sequence":"additional","affiliation":[{"name":"From the Universite\u0301 de Montre\u0301al, Departeme\u0301nt de Physiologie, et l\u2019Institut de Cardiologie de Montre\u0301al, Montre\u0301al, Que\u0301bec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelino","family":"Calderone","sequence":"additional","affiliation":[{"name":"From the Universite\u0301 de Montre\u0301al, Departeme\u0301nt de Physiologie, et l\u2019Institut de Cardiologie de Montre\u0301al, Montre\u0301al, Que\u0301bec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.83.6.1828192"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.75.1.8013079"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.92.9.2385"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3109\/08977198809000251"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1992.262.6.H1861"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703466"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.69.2.1860186"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)35666-1"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1999.276.5.L814"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2980249"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1161\/01.HYP.23.6.1121","volume":"23","year":"1994","unstructured":"Forstermann U, Closs EI, Pollock JS, Nakane M, Schwarz P, Gath I, Kleinert H. 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