{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T19:40:05Z","timestamp":1715542805589},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[1986,12]]},"abstract":"<jats:p>Histamine has been shown to have both positive inotropic and chronotropic effects. To evaluate the chronotropic effects, spontaneously contracting monolayers of cultured rat myocardial cells were treated with histamine, 10(-7) M-10(-4) M. This resulted in a dose-dependent increase in contraction frequency reaching a maximum in 10(-5) M histamine. Contraction frequency (mean +\/- SEM) increased from a control of 121 +\/- 5 contractions per minute to 153 +\/- 4.5, 181 +\/- 9, 212 +\/- 4, and 216 +\/- 1 in 10(-7) M, 10(-6) M, 10(-5) M, and 10(-4) M histamine, respectively (for each n = 10, p less than 0.001). The effect was time-dependent, taking 30 minutes to develop fully. Changes in contraction frequency were accompanied by parallel dose- and time-dependent increases in the verapamil-sensitive sodium influx. Verapamil-sensitive sodium influx (pmol\/cm2\/sec) increased from a control of 10.45 +\/- 1.44 (mean +\/- SEM) to 24.34 +\/- 2.41 and 32.57 +\/- 2.35 at 10- and 30-minute treatment with 10(-6) M histamine (n = 5, p less than 0.001). These data fit the previously described relation between verapamil-sensitive sodium influx and contraction frequency in these cells. Cimetidine (10(-4) M) but not diphenhydramine (10(-4) M) abolished both the contraction frequency and sodium influx response to histamine. Subsequent studies showed a dose- and time-dependent elevation of cyclic adenosine monophosphate (cAMP) with histamine treatment.(ABSTRACT TRUNCATED AT 250 WORDS)<\/jats:p>","DOI":"10.1161\/01.res.59.6.668","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:17:09Z","timestamp":1339460229000},"page":"668-675","update-policy":"http:\/\/dx.doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":7,"title":["The effects of histamine on contraction frequency, sodium influx, and cyclic AMP in cultured rat heart cells."],"prefix":"10.1161","volume":"59","author":[{"given":"D","family":"McCall","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C Y","family":"Lui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Circulation Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.RES.59.6.668","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T19:26:20Z","timestamp":1715541980000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.RES.59.6.668"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,12]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1986,12]]}},"alternative-id":["10.1161\/01.RES.59.6.668"],"URL":"https:\/\/doi.org\/10.1161\/01.res.59.6.668","relation":{},"ISSN":["0009-7330","1524-4571"],"issn-type":[{"value":"0009-7330","type":"print"},{"value":"1524-4571","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,12]]},"assertion":[{"value":"1986-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}