{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T15:30:50Z","timestamp":1758814250945},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[1987,11]]},"abstract":"<jats:p>To clarify the immune mechanism in myocarditis, immunofluorescence techniques with laser flow cytometry were used to examine serial changes in lymphocyte subsets in the heart, spleen, and peripheral blood of DBA\/2 and BALB\/c mice inoculated with encephalomyocarditis virus (Experiment I). B cells were identified by staining with fluorescein isothiocyanate-labelled rabbit anti-mouse immunoglobulin. T-cell subsets were identified with rat anti-Thy 1.2, and nonpolymorphic Lyt 1 and Lyt 2 monoclonal antibodies plus fluorescein isothiocyanate-labelled anti-mouse immunoglobulin. On days 7 and 14 postinfection, the percentage of Thy 1.2+ (pan T) cells in both strains had decreased in the peripheral blood; B cells showed no significant changes throughout the entire period. On the other hand, Thy 1.2+ (pan T) and Lyt 1+, 23+ (precursor and immature) T cells appeared to occupy the major portion of the myocardium on days 7 and 14 when congestive heart failure developed. To confirm this, serial immunohistologic studies (immunoperoxidase staining) of the hearts of DBA\/2 and BALB\/c mice with encephalomyocarditis virus-induced myocarditis were performed (Experiment II). In Experiment II, most of the stained cells in the hearts of both strains were Thy 1.2 positive and Lyt 1 and Lyt 2 positive on days 7 and 14. Thus, Experiments I and II demonstrated that lymphocytes at the site of inflammation in acute viral myocarditis carried antigenic markers that differed from those of peripheral lymphocytes and suggested that Thy 1.2+ (pan T) cells, especially the Lyt 1+, 23+ subset (immature T cells and T-cell subset precursors) were involved in the development of myocarditis in these animals.<\/jats:p>","DOI":"10.1161\/01.res.61.5.715","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:17:09Z","timestamp":1339460229000},"page":"715-725","update-policy":"http:\/\/dx.doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":25,"title":["Immunologic identification of lymphocyte subsets in experimental murine myocarditis with encephalomyocarditis virus. Different kinetics of lymphocyte subsets between the heart and the peripheral blood, and significance of Thy 1.2+ (pan T) and Lyt 1+, 23+ (immature T) subsets in the development of myocarditis."],"prefix":"10.1161","volume":"61","author":[{"given":"C","family":"Kishimoto","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Kuribayashi","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Fukuma","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Masuda","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Tomioka","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W H","family":"Abelmann","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Kawai","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Circulation Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.RES.61.5.715","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T19:35:02Z","timestamp":1715542502000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.RES.61.5.715"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,11]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1987,11]]}},"alternative-id":["10.1161\/01.RES.61.5.715"],"URL":"https:\/\/doi.org\/10.1161\/01.res.61.5.715","relation":{},"ISSN":["0009-7330","1524-4571"],"issn-type":[{"value":"0009-7330","type":"print"},{"value":"1524-4571","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,11]]},"assertion":[{"value":"1987-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}