{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T19:55:29Z","timestamp":1762113329512},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[1987,11]]},"abstract":"<jats:p>Mechanisms of energy deprivation contracture were investigated in cultured chick embryo ventricular cells. In the presence of zero-extracellular-Na+, (choline chloride substitution)-nominal-zero-Ca2+ [( Ca2+] approximately 5 microM), exposure of ventricular cells to 1 mM cyanide (CN) and 20 mM 2-deoxyglucose (2-DG)-zero-glucose solution resulted in the development of a contracture (video motion detector) in 5.9 +\/- 0.5 minutes. Early after contracture development, the resupply of extracellular Na+, in the continued presence of CN + 2-DG, resulted in a rapid partial relaxation (t1\/2 = 1.9 +\/- 0.3 seconds), associated with an increase in 45Ca efflux, presumably due to transsarcolemmal Ca2+ extrusion due to Na+-Ca2+ exchange. Resupply of glucose and removal of CN + 2-DG, in the continued absence of Na+, resulted in an initially slower (t1\/2 = 11.6 +\/- 2.5 seconds), but more complete relaxation of contracture, which was not associated with increased Ca2+ efflux. Pretreatment with 20 mM caffeine delayed the onset of contracture (9.2 +\/- 1.1 minutes) and resulted in a contracture that could not be relaxed by resupply of external Na+ only. Studies using the fluorescent Ca2+ probe indo 1 demonstrated that in zero-Na+-zero-Ca2+ solutions, contracture due to CN + 2-DG was associated with an initial rise in [Ca2+]i but that this did not account for all of contracture force development. In cells exposed to CN + 2-DG in the presence of normal extracellular Na+ and Ca2+ concentrations, a small rise in [Ca2+]i was associated with initial contracture development, consistently preceding the development of a larger accelerated contracture presumably due to ATP depletion.(ABSTRACT TRUNCATED AT 250 WORDS)<\/jats:p>","DOI":"10.1161\/01.res.61.5.726","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:17:09Z","timestamp":1339460229000},"page":"726-734","update-policy":"http:\/\/dx.doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":44,"title":["Role of changes in [Ca2+]i in energy deprivation contracture."],"prefix":"10.1161","volume":"61","author":[{"given":"W H","family":"Barry","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Utah, School of Medicine, Salt Lake City."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G A","family":"Peeters","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Utah, School of Medicine, Salt Lake City."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"C A","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Utah, School of Medicine, Salt Lake City."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M J","family":"Cunningham","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Utah, School of Medicine, Salt Lake City."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Circulation Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.RES.61.5.726","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T19:35:00Z","timestamp":1715542500000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.RES.61.5.726"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,11]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1987,11]]}},"alternative-id":["10.1161\/01.RES.61.5.726"],"URL":"https:\/\/doi.org\/10.1161\/01.res.61.5.726","relation":{},"ISSN":["0009-7330","1524-4571"],"issn-type":[{"value":"0009-7330","type":"print"},{"value":"1524-4571","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,11]]},"assertion":[{"value":"1987-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}