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LTC<jats:sub>4<\/jats:sub>(20 nmol\/L) induced a rapid and significant increase in leukocyte rolling flux that was inhibitable by an anti\u2013P-selectin antibody and soluble sialyl Lewis<jats:sup>x<\/jats:sup>(sLe<jats:sup>x<\/jats:sup>). LTC<jats:sub>4<\/jats:sub>also induced a significant reduction in leukocyte rolling velocity, an event that was independent of P-selectin but entirely dependent on sLe<jats:sup>x<\/jats:sup>. This LTC<jats:sub>4<\/jats:sub>-induced reduction in leukocyte rolling velocity was independent of any hemodynamic alterations. Another P-selectin effector, histamine, did not affect leukocyte rolling velocity even at &gt;5000 times the concentration of LTC<jats:sub>4<\/jats:sub>. Treatment with an anti\u2013L-selectin antibody had no effect on the LTC<jats:sub>4<\/jats:sub>-induced increase in leukocyte rolling or reduction in rolling velocity. Inhibition of LTC<jats:sub>4<\/jats:sub>bioconversion to LTD<jats:sub>4<\/jats:sub>by pretreatment with<jats:sc>l<\/jats:sc>-serine (100 \u03bcmol\/L) prevented the LTC<jats:sub>4<\/jats:sub>-induced increase in leukocyte rolling flux and the LTC<jats:sub>4<\/jats:sub>-induced reduction in leukocyte rolling velocity. A subtle, yet significant, increase in leukocyte adhesion was also observed with LTC<jats:sub>4<\/jats:sub>. Pretreatment with a platelet-activating factor receptor antagonist returned the LTC<jats:sub>4<\/jats:sub>-induced leukocyte rolling velocity to baseline levels. The addition of a very low concentration of platelet-activating factor (1 nmol\/L) induced significant leukocyte adhesion in the presence of LTC<jats:sub>4<\/jats:sub>but not histamine. This study demonstrates that LTC<jats:sub>4<\/jats:sub>, via bioconversion to leukotriene D<jats:sub>4<\/jats:sub>, induces a P-selectin\u2013dependent and sLe<jats:sup>x<\/jats:sup>-dependent increase in leukocyte rolling flux and a P-selectin\u2013independent but sLe<jats:sup>x<\/jats:sup>-dependent reduction in leukocyte rolling velocity, a parameter that may play an essential role in subsequent leukocyte adhesion.<\/jats:p>","DOI":"10.1161\/01.res.77.5.879","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:47:05Z","timestamp":1339462025000},"page":"879-887","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":67,"title":["Leukotriene C<sub>4<\/sub>\/D<sub>4<\/sub>Induces P-Selectin and Sialyl Lewis<sup>x<\/sup>\u2013Dependent Alterations in Leukocyte Kinetics In Vivo"],"prefix":"10.1161","volume":"77","author":[{"given":"Samina","family":"Kanwar","sequence":"first","affiliation":[{"name":"From the Immunology Research Group, University of Calgary (Canada)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brent","family":"Johnston","sequence":"additional","affiliation":[{"name":"From the Immunology Research Group, University of Calgary (Canada)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Kubes","sequence":"additional","affiliation":[{"name":"From the Immunology Research Group, University of Calgary (Canada)."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0952-7915(89)90093-9"},{"key":"e_1_3_2_2_2","first-page":"13","volume":"29","year":"1989","unstructured":"Schutzer K-M, Haglund U, Falk A. 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