{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T03:57:17Z","timestamp":1783569437462,"version":"3.55.0"},"reference-count":47,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[1995,11]]},"abstract":"<jats:p><jats:italic>Abstract<\/jats:italic>In vitro evidence suggests that natriuretic peptide receptors (NPR)-B and NPR-C inhibit vascular smooth muscle (VSM) proliferation. NPR-B is guanylate cyclase-coupled and selectively activated by C-type natriuretic peptide (CNP)-(1-22). NPR-C is not guanylate cyclase-coupled and, unlike NPR-B, avidly binds atrial natriuretic peptide (ANP)-(1-28) as well as CNP-(1-22). Here, we investigate these receptors during the VSM proliferation and neointimal formation found 5, 7, and 20 days after compressing the central ear artery of the rabbit. Receptors were mapped autoradiographically using [<jats:sup>125<\/jats:sup>I-Tyr<jats:sup>0<\/jats:sup>]CNP-(1-22), which binds NPR-B and NPR-C, and<jats:sup>125<\/jats:sup>I-ANP-(1-28), which binds NPR-C and NPR-A, another guanylate cyclase-coupled receptor. Normal tunica media had NPR-B\u2013like binding sites, and the level of these did not change significantly after compression. Consistent with this, CNP-(1-22) stimulated cGMP production equally with membranes from normal or damaged arteries and was more effective than ANP-(1-28). Neointima, which became evident 5 to 7 days after arterial damage, expressed NPR-C\u2013like sites and no detectable NPR-B\u2013like binding. NPR-C\u2013like sites also appeared on the media for the first time between 5 and 7 days after compression. Immunohistochemistry for proliferating cell nuclear antigen revealed widespread mitosis in VSM at 5 days after compression, but mitosis was virtually restricted to the neointima at and beyond 7 days after compression. Thus, whereas levels of NPR-B did not change significantly after arterial injury and NPR-A was not detected, NPR-C\u2013like receptors were unregulated as mitosis declined in the media and as a prominent neointima formed.<\/jats:p>","DOI":"10.1161\/01.res.77.5.906","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:47:05Z","timestamp":1339462025000},"page":"906-918","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":29,"title":["Regional Expression of Natriuretic Peptide Receptors During the Formation of Arterial Neointima in the Rabbit"],"prefix":"10.1161","volume":"77","author":[{"given":"J.","family":"Brown","sequence":"first","affiliation":[{"name":"From the Physiological Laboratory, Cambridge, England."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Q.","family":"Chen","sequence":"additional","affiliation":[{"name":"From the Physiological Laboratory, Cambridge, England."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","first-page":"1","volume":"45","year":"1993","unstructured":"Bobick A, Campbell J. 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