{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T05:50:17Z","timestamp":1761630617868},"reference-count":45,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[1999,1,22]]},"abstract":"<jats:p>\n            <jats:italic>Abstract<\/jats:italic>\n            \u2014Traumatic shock induces profound pathophysiological alterations and initiates inflammatory reactions in many tissues, thus resulting in acute multiple organ damage (eg, intestine, pancreas, and liver). In the rat, Noble-Collip drum trauma increases P-selectin expression on the vascular endothelium as a result of loss of endothelium-derived NO. Here we postulated that blockade of the earliest steps in leukocyte adhesion (ie, leukocyte rolling) via administration of a recombinant soluble form of P-selectin glycoprotein ligand-1 (PSGL-1; the recombinant soluble form is rsPSGL.Ig) would attenuate selectin-mediated events observed in the rat during traumatic shock. Using intravital microscopy of the rat mesenteric microvasculature, we found that intravenous infusion of rsPSGL.Ig significantly inhibited leukocyte-endothelium interaction (ie, leukocyte rolling, adherence, and transmigration) induced by traumatic shock as well as by activation of the microvascular endothelium with 50 \u03bcmol\/L\n            <jats:italic>N<\/jats:italic>\n            <jats:sup>G<\/jats:sup>\n            -nitro-\n            <jats:sc>l<\/jats:sc>\n            -arginine methyl ester. Immunohistochemical detection of P-selectin on the mesenteric venular endothelial surface demonstrated that rsPSGL.Ig functionally neutralizes effects of P-selectin on the endothelial cell surface rather than attenuating P-selectin expression. Systemic administration of rsPSGL.Ig to traumatized rats prolonged survival time and survival rate, significantly attenuating ileal myeloperoxidase activity and significantly preserving mesenteric endothelial function. Furthermore, PSGL-1 mRNA levels were significantly increased in the blood of traumatized rats and were reduced after systemic administration of rsPSGL.Ig. Thus, soluble recombinant forms of PSGL-1 are able to ameliorate acute shock states by suppressing selectin-mediated leukocyte-endothelium interaction at both the functional and molecular levels.\n          <\/jats:p>","DOI":"10.1161\/01.res.84.1.93","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:47:05Z","timestamp":1339462025000},"page":"93-102","update-policy":"http:\/\/dx.doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":25,"title":["Effect of Recombinant Soluble P-Selectin Glycoprotein Ligand-1 on Leukocyte-Endothelium Interaction In Vivo"],"prefix":"10.1161","volume":"84","author":[{"given":"Rosario","family":"Scalia","sequence":"first","affiliation":[{"name":"From the Department of Physiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pa."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reid","family":"Hayward","sequence":"additional","affiliation":[{"name":"From the Department of Physiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pa."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerie E.","family":"Armstead","sequence":"additional","affiliation":[{"name":"From the Department of Physiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pa."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander G.","family":"Minchenko","sequence":"additional","affiliation":[{"name":"From the Department of Physiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pa."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allan M.","family":"Lefer","sequence":"additional","affiliation":[{"name":"From the Department of Physiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pa."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.1942.sp000856"},{"key":"e_1_3_2_2_2","first-page":"19","volume":"1","year":"1994","unstructured":"Christopher TA, Ma XL, Lefer AM. Beneficial actions of S-nitroso-N-acetylpenicillamine, a nitric oxide donor, in murine traumatic shock. Shock. 1994;1:19\u201324.","journal-title":"Shock"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-198811000-00002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0955-0674(92)90109-P"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.33.040193.000443"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-199609010-00004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116623"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.118.2.445"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)31656-3"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.128.4.661"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90278-3"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90327-M"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119457"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90173-6"},{"key":"e_1_3_2_15_2","first-page":"16794","volume":"269","year":"1994","unstructured":"Natsuka S, Gersten KM, Zenita K, Kannagi R, Lowe JB. Molecular cloning of a cDNA encoding a novel human leukocyte \u03b1-1,3-fucosyltransferase capable of synthesizing the sialyl Lewisx determinant. J Biol Chem. 1994;269:16794\u201316799.","journal-title":"J Biol Chem"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.19.9326"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.78.5.780"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.42.5.455"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.18.9967"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0026-2862(84)90047-5"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1989.257.5.G683"},{"key":"e_1_3_2_22_2","first-page":"54","volume":"75","year":"1995","unstructured":"Weyrich AS, Burke M, Albertine KH, Lefer AM. Time course of coronary vascular endothelial adhesion molecules during reperfusion of the ischemic feline myocardium. 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Superoxide mediates reperfusion-induced leukocyte-endothelial cell interactions. Am J Physiol. 1989;257:H1740\u2013H1745.","journal-title":"Am J Physiol"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198902093200606"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V86.12.4417.bloodjournal86124417"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118234"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.7.2415.bloodjournal8872415"},{"key":"e_1_3_2_42_2","doi-asserted-by":"crossref","first-page":"3995","DOI":"10.4049\/jimmunol.157.9.3995","volume":"157","year":"1996","unstructured":"Tu LL, Chen AJ, Delahunty MD, Moore KL, Watson SR, McEver RP, Tedder TF. L-selectin binds to P-selectin glycoprotein ligand-1 on leukocytes: interactions between the lectin, epidermal growth factor, and consensus repeat domains of the selectins determine ligand binding specificity. 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