{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:45:56Z","timestamp":1790858756491,"version":"4.1.0"},"reference-count":24,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[1999,4,2]]},"abstract":"<jats:p>\n            <jats:italic>Abstract<\/jats:italic>\n            \u2014The laminins belong to a family of trimeric basement membrane glycoproteins with multiple domains, structures, and functions. Endothelial cells bind laminin-1 and form capillary-like structures when plated on a laminin-1\u2013rich basement membrane matrix, Matrigel. Laminin-1 is composed of 3 chains, \u03b11, \u03b21, and \u03b31. Because laminin-1 is known to contain multiple biologically active sites, we have screened 156 synthetic overlapping peptides spanning the entire laminin \u03b31 chain for potential angiogenic sequences. Only 7 of these peptides, designated as C16, C25, C30, C38, C64, C75, and C102, disrupted the formation of capillary-like structures by human umbilical vein endothelial cells on Matrigel. Dose-response experiments in the presence of 50 to 200 \u03bcg\/mL showed that tube formation was prevented by most peptides at 150 and 200 \u03bcg\/mL, except for C16, which showed strong activity at all concentrations. Active peptides promoted vessel sprouting from aorta rings and angiogenesis in the chick chorioallantoic membrane assay. In addition, the active peptides also promoted endothelial cell adhesion to dishes coated with 0.1 \u03bcg of peptide and inhibited attachment to laminin-1 but not to plastic or fibronectin. Four of the active peptides, C25, C38, C75, and C102, may have cell-type specificity with endothelial cells, since they did not promote PC12 neurite outgrowth or adhesion of B16-F10 melanoma and human submandibular gland cells. These results suggest that specific laminin \u03b31\u2013chain peptides have angiogenic activity with potential therapeutic applications.\n          <\/jats:p>","DOI":"10.1161\/01.res.84.6.688","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:47:05Z","timestamp":1339462025000},"page":"688-694","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":83,"title":["Identification of Endothelial Cell Binding Sites on the Laminin \u03b31 Chain"],"prefix":"10.1161","volume":"84","author":[{"given":"M. Lourdes","family":"Ponce","sequence":"first","affiliation":[{"name":"From the Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motoyoshi","family":"Nomizu","sequence":"additional","affiliation":[{"name":"From the Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mucio C.","family":"Delgado","sequence":"additional","affiliation":[{"name":"From the Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichiro","family":"Kuratomi","sequence":"additional","affiliation":[{"name":"From the Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew P.","family":"Hoffman","sequence":"additional","affiliation":[{"name":"From the Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"Powell","sequence":"additional","affiliation":[{"name":"From the Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiko","family":"Yamada","sequence":"additional","affiliation":[{"name":"From the Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hynda K.","family":"Kleinman","sequence":"additional","affiliation":[{"name":"From the Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine M.","family":"Malinda","sequence":"additional","affiliation":[{"name":"From the Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0945-053X(94)90192-9"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0955-0674(90)90082-P"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0945-053X(94)90184-8"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.145.1.204"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83607-4"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.4.1589"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041530324"},{"key":"e_1_3_2_8_2","first-page":"903","volume":"51","year":"1991","unstructured":"Sakamoto N, Iwahana M, Tanaka NG, Osada T. Inhibition of angiogenesis and tumor growth by a synthetic laminin peptide, CDPGYIGSR-NH2. Cancer Res. 1991;51:903\u2013906.","journal-title":"Cancer Res"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/84.21.1633"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.51.32198"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0083-6729(08)60446-X"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.4.2.2404817"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1996.0304"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00350a005"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)45498-1"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.35.20583"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107470"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.109.8.2013"},{"key":"e_1_3_2_19_2","first-page":"115","volume":"63","year":"1990","unstructured":"Nicosia RF, Ottinetti A. Growth of microvessels in serum-free matrix culture of rat aorta: a quantitative assay of angiogenesis in vitro. Lab Invest. 1990;63:115\u2013122.","journal-title":"Lab Invest"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90945-8"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5323.225"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.13.1.53"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb05836.x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06683.x"}],"container-title":["Circulation Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.RES.84.6.688","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T20:20:16Z","timestamp":1715545216000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.RES.84.6.688"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,4,2]]},"references-count":24,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1999,4,2]]}},"alternative-id":["10.1161\/01.RES.84.6.688"],"URL":"https:\/\/doi.org\/10.1161\/01.res.84.6.688","relation":{},"ISSN":["0009-7330","1524-4571"],"issn-type":[{"value":"0009-7330","type":"print"},{"value":"1524-4571","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,4,2]]},"assertion":[{"value":"1998-08-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-01-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-04-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}