{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:55:09Z","timestamp":1791114909370,"version":"4.1.0"},"reference-count":20,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"12","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2000,6,23]]},"abstract":"<jats:p>\n                    <jats:italic>Abstract<\/jats:italic>\n                    \u2014Lymphocyte binding to vascular endothelium is a prerequisite for the movement of immune cells from the blood into lymphoid tissues and into sites of inflammation. Human vascular adhesion protein-1 (VAP-1) is an endothelial glycoprotein involved in this interaction. It also displays an enzymatic (monoamine oxidase) activity. Here we examined how recombinant human VAP-1 mediates lymphocyte binding using rotatory and flow chamber binding assays. VAP-1 cDNA transfected into an endothelial cell line, which does not bind lymphocytes, renders the cell line capable of binding lymphocytes in a shear-dependent manner. VAP-1 transfectants bound lymphocytes 5 times better than monocytes with a preference for T killer cells, and no specific granulocyte adherence was detectable. The binding is partially inhibited by anti\u2013VAP-1 monoclonal antibodies or by blocking lymphocyte L-selectin and CD18 integrins, but not by inhibition of several other homing-associated molecules. In contrast, CD44 ligation on lymphocytes markedly upregulates their VAP-1\u2013dependent adhesion, suggesting that the VAP-1 counterreceptor can be activated via CD44. The transfectant model also allowed us to perform detailed structure-function analyses of VAP-1. We show that the exposed integrin-binding motif RGD or the enzymatic activity is not indispensable for VAP-1\u2013dependent adhesion. Together, these data show that VAP-1 can reconstitute the lymphocyte-endothelial adhesion cascade under shear and propose a critical role for VAP-1 in lymphocyte emigration from the blood.\n                  <\/jats:p>","DOI":"10.1161\/01.res.86.12.1245","type":"journal-article","created":{"date-parts":[[2012,6,11]],"date-time":"2012-06-11T20:52:16Z","timestamp":1339447936000},"page":"1245-1251","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":37,"title":["Human Vascular Adhesion Protein-1 (VAP-1) Plays a Critical Role in Lymphocyte\u2013Endothelial Cell Adhesion Cascade Under Shear"],"prefix":"10.1161","volume":"86","author":[{"given":"Marko","family":"Salmi","sequence":"first","affiliation":[{"name":"From the MediCity Research Laboratory, University of Turku, and National Public Health Institute, Department in Turku, Turku, Finland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sami","family":"Tohka","sequence":"additional","affiliation":[{"name":"From the MediCity Research Laboratory, University of Turku, and National Public Health Institute, Department in Turku, Turku, Finland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirpa","family":"Jalkanen","sequence":"additional","affiliation":[{"name":"From the MediCity Research Laboratory, University of Turku, and National Public Health Institute, Department in Turku, Turku, Finland."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60889-5"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90337-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5258.60"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1529341"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(95)80023-9"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/1357-2725(95)00130-1"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.63.070194.001503"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.1.17"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.144.3.828"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830180814"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.23.11436"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.4.589"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.2.241"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00834-0"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830270730"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.161.3.1549"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.2.927"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830230826"},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"4399","DOI":"10.4049\/jimmunol.153.10.4399","volume":"153","year":"1994","unstructured":"Galandrini R, De Maria R, Piccoli M, Frati L, Santoni A. CD44 triggering enhances human NK cell cytotoxic functions. J Immunol. 1994;153:4399\u20134407.","journal-title":"J Immunol"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/11.12.1195"}],"container-title":["Circulation Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.RES.86.12.1245","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T16:34:13Z","timestamp":1715531653000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.RES.86.12.1245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,6,23]]},"references-count":20,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2000,6,23]]}},"alternative-id":["10.1161\/01.RES.86.12.1245"],"URL":"https:\/\/doi.org\/10.1161\/01.res.86.12.1245","relation":{},"ISSN":["0009-7330","1524-4571"],"issn-type":[{"value":"0009-7330","type":"print"},{"value":"1524-4571","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,6,23]]},"assertion":[{"value":"1999-12-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-04-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-06-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}