{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:04:57Z","timestamp":1791313497987,"version":"4.3.1"},"reference-count":25,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2000,3,3]]},"abstract":"<jats:p>\n            <jats:italic>Abstract<\/jats:italic>\n            \u2014The cytoskeleton of the cardiomyocyte has been shown to modulate ion channel function. Cytoskeletal disruption in vitro alters Na\n            <jats:sup>+<\/jats:sup>\n            channel kinetics, producing a late Na\n            <jats:sup>+<\/jats:sup>\n            current that can prolong repolarization. This study describes the properties of the cardiac Na\n            <jats:sup>+<\/jats:sup>\n            channel and cardiac repolarization in neonatal mice lacking ankyrin\n            <jats:sub>B<\/jats:sub>\n            , a cytoskeletal \u201cadaptor\u201d protein. Using whole-cell voltage clamp techniques,\n            <jats:italic>I<\/jats:italic>\n            <jats:sub>Na<\/jats:sub>\n            density was lower in ankyrin\n            <jats:sub>B<\/jats:sub>\n            (\u2212\/\u2212) ventricular myocytes than in wild-type (WT) myocytes (\u2212307\u00b126 versus \u2212444\u00b139 pA\/pF,\n            <jats:italic>P<\/jats:italic>\n            &lt;0.01). Ankyrin\n            <jats:sub>B<\/jats:sub>\n            (\u2212\/\u2212) myocytes exhibited a hyperpolarizing shift in activation and inactivation kinetics compared with WT. Slower recovery from inactivation contributed to the negative shift in steady-state inactivation in ankyrin\n            <jats:sub>B<\/jats:sub>\n            (\u2212\/\u2212). Single Na\n            <jats:sup>+<\/jats:sup>\n            channel mean open time was longer in ankyrin\n            <jats:sub>B<\/jats:sub>\n            (\u2212\/\u2212) versus WT at test potentials (\n            <jats:italic>V<\/jats:italic>\n            <jats:sub>t<\/jats:sub>\n            ) of \u221240 mV (1.0\u00b10.1 versus 0.61\u00b10.04 ms,\n            <jats:italic>P<\/jats:italic>\n            &lt;0.05) and \u221250 mV (0.8\u00b10.1 versus 0.39\u00b10.05 ms,\n            <jats:italic>P<\/jats:italic>\n            &lt;0.05). Ankyrin\n            <jats:sub>B<\/jats:sub>\n            (\u2212\/\u2212) exhibited late single-channel openings at\n            <jats:italic>V<\/jats:italic>\n            <jats:sub>t<\/jats:sub>\n            \u221240 and \u221250 mV, which were not seen in WT. Late\n            <jats:italic>I<\/jats:italic>\n            <jats:sub>Na<\/jats:sub>\n            contributed to longer action potential durations measured at 90% repolarization (APD\n            <jats:sub>90<\/jats:sub>\n            ) at 1 Hz stimulation in ankyrin\n            <jats:sub>B<\/jats:sub>\n            (\u2212\/\u2212) compared with WT (354\u00b126 versus 274\u00b122 ms,\n            <jats:italic>P<\/jats:italic>\n            &lt;0.05). From ECG recordings of neonatal mice, heart rates were slower in ankyrin\n            <jats:sub>B<\/jats:sub>\n            (\u2212\/\u2212) than in WT (380\u00b114 versus 434\u00b113 bpm,\n            <jats:italic>P<\/jats:italic>\n            &lt;0.01). Although the QT interval was similar in ankyrin\n            <jats:sub>B<\/jats:sub>\n            (\u2212\/\u2212) and WT at physiological heart rates, QT-interval prolongation in response to heart rate deceleration was greater in ankyrin\n            <jats:sub>B<\/jats:sub>\n            (\u2212\/\u2212). In conclusion, Na\n            <jats:sup>+<\/jats:sup>\n            channels in ankyrin\n            <jats:sub>B<\/jats:sub>\n            (\u2212\/\u2212) display reduced\n            <jats:italic>I<\/jats:italic>\n            <jats:sub>Na<\/jats:sub>\n            density and abnormal kinetics at the whole-cell and single-channel level that contribute to prolonged APD\n            <jats:sub>90<\/jats:sub>\n            and abnormal QT-rate adaptation.\n          <\/jats:p>","DOI":"10.1161\/01.res.86.4.441","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:52:16Z","timestamp":1339462336000},"page":"441-447","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":76,"title":["Abnormal Cardiac Na\n            <sup>+<\/sup>\n            Channel Properties and QT Heart Rate Adaptation in Neonatal Ankyrin\n            <sub>B<\/sub>\n            Knockout Mice"],"prefix":"10.1161","volume":"86","author":[{"given":"Vijay S.","family":"Chauhan","sequence":"first","affiliation":[{"name":"From the Division of Cardiology (V.S.C., A.O.G.), Duke University Medical Center, and Department of Cell Biology (S.T., M.B., V.B.), Howard Hughes Medical Institute, Durham, NC."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shmuel","family":"Tuvia","sequence":"additional","affiliation":[{"name":"From the Division of Cardiology (V.S.C., A.O.G.), Duke University Medical Center, and Department of Cell Biology (S.T., M.B., V.B.), Howard Hughes Medical Institute, Durham, NC."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona","family":"Buhusi","sequence":"additional","affiliation":[{"name":"From the Division of Cardiology (V.S.C., A.O.G.), Duke University Medical Center, and Department of Cell Biology (S.T., M.B., V.B.), Howard Hughes Medical Institute, Durham, NC."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vann","family":"Bennett","sequence":"additional","affiliation":[{"name":"From the Division of Cardiology (V.S.C., A.O.G.), Duke University Medical Center, and Department of Cell Biology (S.T., M.B., V.B.), Howard Hughes Medical Institute, Durham, NC."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augustus O.","family":"Grant","sequence":"additional","affiliation":[{"name":"From the Division of Cardiology (V.S.C., A.O.G.), Duke University Medical Center, and Department of Cell Biology (S.T., M.B., V.B.), Howard Hughes Medical Institute, Durham, NC."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.09.110193.000331"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.4.1008"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.147.5.995"},{"key":"e_1_3_2_4_2","first-page":"H203","volume":"269","year":"1995","unstructured":"Undrovinas AI, Shander GS, Makielski JC. 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Am J Hum Genet. 1995;57:1114\u20131122.","journal-title":"Am J Hum Genet"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.88.2.8339437"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1540-8167.1998.tb00096.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.96.5.1551"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.83.1.95"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.83.6.668"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.83.5.560"}],"container-title":["Circulation Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.RES.86.4.441","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T20:26:20Z","timestamp":1715545580000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.RES.86.4.441"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3,3]]},"references-count":25,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2000,3,3]]}},"alternative-id":["10.1161\/01.RES.86.4.441"],"URL":"https:\/\/doi.org\/10.1161\/01.res.86.4.441","relation":{},"ISSN":["0009-7330","1524-4571"],"issn-type":[{"value":"0009-7330","type":"print"},{"value":"1524-4571","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,3,3]]},"assertion":[{"value":"1999-09-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-12-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-03-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}