{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T07:38:49Z","timestamp":1785224329556,"version":"3.55.0"},"reference-count":46,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2000,10,27]]},"abstract":"<jats:p>\n            <jats:italic>Abstract<\/jats:italic>\n            \u2014We sought to determine whether adenovirus-mediated gene transfer in vivo of calcitonin gene\u2013related peptide (CGRP), a potent vasodilator, ameliorates cerebral vasoconstriction after experimental subarachnoid hemorrhage (SAH). Arterial blood was injected into the cisterna magna of rabbits to mimic SAH 5 days after injection of AdRSVCGRP (8\u00d710\n            <jats:sup>8<\/jats:sup>\n            pfu), AdRSV\u03b2gal (control virus), or vehicle. After injection of AdRSVCGRP, there was a 400-fold increase in CGRP in cerebrospinal fluid. Contraction of the basilar artery to serotonin in vitro was greater in rabbits after SAH than after injection of artificial cerebrospinal fluid (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.001). Contraction to serotonin was less in rabbits with SAH after AdRSVCGRP than after AdRSV\u03b2gal or vehicle (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.02). Basal diameter of the basilar artery before SAH (measured with digital subtraction angiogram) was 13% greater in rabbits treated with AdRSVCGRP than in rabbits treated with vehicle or AdRSV\u03b2gal (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.005). In rabbits treated with vehicle or AdRSV\u03b2gal, arterial diameter after SAH was 25\u00b13% smaller than before SAH (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.0005). In rabbits treated with AdRSVCGRP, arterial diameter was similar before and after SAH and was reduced by 19\u00b13% (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.01) after intracisternal injection of CGRP-(8-37) (0.5 nmol\/kg), a CGRP\n            <jats:sub>1<\/jats:sub>\n            receptor antagonist. To determine whether gene transfer of CGRP after SAH may prevent cerebral vasoconstriction, we constructed a virus with a cytomegalovirus (CMV) promoter, which results in rapid expression of the transgene product. Treatment of rabbits with AdCMVCGRP after experimental SAH prevented constriction of the basilar artery 2 days after SAH. Thus, gene transfer of CGRP prevents cerebral vasoconstriction in vivo after experimental SAH.\n          <\/jats:p>","DOI":"10.1161\/01.res.87.9.818","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:52:16Z","timestamp":1339462336000},"page":"818-824","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":51,"title":["Gene Transfer of Calcitonin Gene\u2013Related Peptide Prevents Vasoconstriction After Subarachnoid Hemorrhage"],"prefix":"10.1161","volume":"87","author":[{"given":"Kazunori","family":"Toyoda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank M.","family":"Faraci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshimasa","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiro","family":"Ueda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jon J.","family":"Andresen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoichiro","family":"Otake","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald D.","family":"Heistad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","unstructured":"Mohr JP Kistler JP Fink ME. 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