{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T03:40:02Z","timestamp":1784173202485,"version":"3.55.0"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2001,2,2]]},"abstract":"<jats:p>\n            <jats:italic>Abstract<\/jats:italic>\n            \u2014Changes in the behavior of the sarcoplasmic reticulum (SR) in rat ventricular myocytes were investigated under conditions of metabolic inhibition using laser-scanning confocal microscopy to measure intracellular Ca\n            <jats:sup>2+<\/jats:sup>\n            and the perforated patch-clamp technique to measure SR Ca\n            <jats:sup>2+<\/jats:sup>\n            content. Metabolic inhibition had several effects on SR function, including reduced frequency of spontaneous releases of Ca\n            <jats:sup>2+<\/jats:sup>\n            (sparks and waves of Ca\n            <jats:sup>2+<\/jats:sup>\n            -induced Ca\n            <jats:sup>2+<\/jats:sup>\n            release), increased SR Ca\n            <jats:sup>2+<\/jats:sup>\n            content (79.4\u00b15.7 to 115.2\u00b16.6 \u03bcmol\/L cell volume [mean\u00b1SEM;\n            <jats:italic>P<\/jats:italic>\n            &lt;0.001]), and, after a wave of Ca\n            <jats:sup>2+<\/jats:sup>\n            release, slower reuptake of Ca\n            <jats:sup>2+<\/jats:sup>\n            into the SR (rate constant of fall of Ca\n            <jats:sup>2+<\/jats:sup>\n            reduced from 8.5\u00b11.1 s\n            <jats:sup>\u2212<\/jats:sup>\n            <jats:sup>1<\/jats:sup>\n            in control to 5.2\u00b10.4 s\n            <jats:sup>\u2212<\/jats:sup>\n            <jats:sup>1<\/jats:sup>\n            in metabolic inhibition [\n            <jats:italic>P<\/jats:italic>\n            &lt;0.01]). Inhibition of L-type Ca\n            <jats:sup>2+<\/jats:sup>\n            channels with Cd\n            <jats:sup>2+<\/jats:sup>\n            (100 \u03bcmol\/L) did not reproduce the effects of metabolic inhibition on spontaneous Ca\n            <jats:sup>2+<\/jats:sup>\n            sparks. These results are evidence of inhibition of both Ca\n            <jats:sup>2+<\/jats:sup>\n            release and reuptake mechanisms. Reduced frequency of release could be attributable to either of these effects, but the increased SR Ca\n            <jats:sup>2+<\/jats:sup>\n            content at the time of reduced frequency of spontaneous release of Ca\n            <jats:sup>2+<\/jats:sup>\n            shows that the dominant effect of metabolic inhibition is to inhibit release of Ca\n            <jats:sup>2+<\/jats:sup>\n            from the SR, allowing the accumulation of greater than normal amounts of Ca\n            <jats:sup>2+<\/jats:sup>\n            . In the context of ischemia, this extra accumulation of Ca\n            <jats:sup>2+<\/jats:sup>\n            would present a risk of potentially arrhythmogenic, spontaneous release of Ca\n            <jats:sup>2+<\/jats:sup>\n            on reperfusion of the tissue.\n          <\/jats:p>","DOI":"10.1161\/01.res.88.2.181","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:52:16Z","timestamp":1339462336000},"page":"181-187","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":35,"title":["Altered Cardiac Sarcoplasmic Reticulum Function of Intact Myocytes of Rat Ventricle During Metabolic Inhibition"],"prefix":"10.1161","volume":"88","author":[{"given":"C. L.","family":"Overend","sequence":"first","affiliation":[{"name":"From the Department of Medicine, University of Manchester, Manchester, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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