{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:21:17Z","timestamp":1787062877125,"version":"build-2736575974"},"reference-count":38,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:p>\n                    <jats:bold>\n                      <jats:italic>Background and Purpose\u2014<\/jats:italic>\n                    <\/jats:bold>\n                    In ischemic stroke, the ischemic crisis activates a cascade of events that are potentiated by reperfusion, eventually leading to cell death. The chief aim in this study was to investigate whether our new experimental model for stroke therapy, flushing the ischemic territory with saline before reperfusion, could minimize this damage by (1) reducing the inflammatory reaction and (2) improving regional microcirculation.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>\n                      <jats:italic>Methods\u2014<\/jats:italic>\n                    <\/jats:bold>\n                    Stroke in Sprague-Dawley rats (n=39) was induced by a 2-hour middle cerebral artery occlusion with the use of a novel intraluminal hollow filament. Before 48-hour reperfusion, 20 of the ischemic rats received 7 mL isotonic saline at 23\u00b0C or 37\u00b0C infused into the ischemic area through the filament. Regional cerebral blood flow in cortex supplied by the right middle cerebral artery was measured by laser-Doppler flowmetry during ischemia and reperfusion. Leukocyte infiltration, microvascular plugging, and infarct volume were compared with the use of hematoxylin and eosin staining. Expression of intercellular adhesion molecule 1 (ICAM-1) was determined by immunocytochemistry. Neurological deficits were evaluated.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>\n                      <jats:italic>Results\u2014<\/jats:italic>\n                    <\/jats:bold>\n                    After the prereperfusion infusion of saline, significantly (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.001) improved cerebral blood flow (105\u00b112% of baseline) was obtained up to 48 hours after reperfusion, compared with 45\u00b17% at 24 hours and 25\u00b13% at 48 hours after reperfusion without local saline infusion. Significant (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.001) reductions in leukocyte infiltration (61%), vascular plugging (45%), infarct volume (approximately 65%), and neurological deficits were also produced. ICAM-1 expression in the infarct region was significantly (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.05) minimized by 37%.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>\n                      <jats:italic>Conclusions\u2014<\/jats:italic>\n                    <\/jats:bold>\n                    The reduced brain infarct and neurological deficits may be attributed to adequate reperfusion and ameliorated inflammation induced by local prereperfusion infusion.\n                  <\/jats:p>","DOI":"10.1161\/01.str.0000028237.15541.cc","type":"journal-article","created":{"date-parts":[[2002,10,3]],"date-time":"2002-10-03T18:27:27Z","timestamp":1033669647000},"page":"2492-2498","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":88,"title":["Prereperfusion Saline Infusion Into Ischemic Territory Reduces Inflammatory Injury After Transient Middle Cerebral Artery Occlusion in Rats"],"prefix":"10.1161","volume":"33","author":[{"given":"Yuchuan","family":"Ding","sequence":"first","affiliation":[{"name":"From the Departments of Neurological Surgery (Y.D., J.L., F.G.D.), Anatomy and Cell Biology (J.A.R.), and Physiology (J.W.P.), Wayne State University School of Medicine, Detroit, Mich."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"From the Departments of Neurological Surgery (Y.D., J.L., F.G.D.), Anatomy and Cell Biology (J.A.R.), and Physiology (J.W.P.), Wayne State University School of Medicine, Detroit, Mich."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0300 A.","family":"Rafols","sequence":"additional","affiliation":[{"name":"From the Departments of Neurological Surgery (Y.D., J.L., F.G.D.), Anatomy and Cell Biology (J.A.R.), and Physiology (J.W.P.), Wayne State University School of Medicine, Detroit, Mich."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Phillis","sequence":"additional","affiliation":[{"name":"From the Departments of Neurological Surgery (Y.D., J.L., F.G.D.), Anatomy and Cell Biology (J.A.R.), and Physiology (J.W.P.), Wayne State University School of Medicine, Detroit, Mich."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando G.","family":"Diaz","sequence":"additional","affiliation":[{"name":"From the Departments of Neurological Surgery (Y.D., J.L., F.G.D.), Anatomy and Cell Biology (J.A.R.), and Physiology (J.W.P.), Wayne State University School of Medicine, Detroit, Mich."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","first-page":"58","volume":"2","year":"1990","unstructured":"Ginsberg MD. 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Reperfusable brain infarction model in rat.In: Proceedings of the 10th Meeting of the Japanese Stroke Society; 1985.Abstract."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.20.1.2643202"},{"key":"e_1_3_2_21_2","unstructured":"Mohr JP Gautier JC Hier D Stein RW. Stroke: pathophysiology diagnosis and management. In: Barnett HJM Stein BM Mohr JP Yatsu FM eds. Stroke: Pathophysiology Diagnosis and Management. New York NY: Churchill Livingstone 1986: 377\u2013450."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0049-3848(10)80003-9"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-199708000-00004"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.2002.96.2.0310"},{"key":"e_1_3_2_25_2","first-page":"1477","volume":"147","year":"1995","unstructured":"Garcia JH, Liu KF, Relton JK. Interleukin-1 receptor antagonist decreases the number of necrotic neurons in rats with middle cerebral artery occlusion. Am J Pathol. 1995; 147: 1477\u20131486.","journal-title":"Am J Pathol"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.25.1.7505494"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(94)90074-4"},{"key":"e_1_3_2_28_2","first-page":"188","volume":"144","year":"1994","unstructured":"Garcia JH, Liu KF, Yoshida Y, Lian J, Chen S, del Zoppo GJ. Influx of leukocytes and platelets in an evolving brain infarct (Wistar rat). Am J Pathol. 1994; 144: 188\u2013199.","journal-title":"Am J Pathol"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(94)90234-8"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-197407000-00007"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1990.47"},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","unstructured":"Bivin WS Crawford MP Brewer NR. Morphophysiology.In: Baker HJ Lindsey JR Weisbroth SH eds. The Laboratory Rat. 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