{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T22:44:14Z","timestamp":1759099454003},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2004,9]]},"abstract":"\n \n Background and Purpose\u2014<\/jats:italic>\n <\/jats:bold>\n All studies reporting high numbers of\n Chlamydia pneumoniae<\/jats:italic>\n DNA positives in stroke patients published to date have used polymerase chain reaction (PCR) techniques highly prone to generate false-positive results. The aim of this study was to analyze the prevalence of\n C. pneumoniae<\/jats:italic>\n DNA in plaques of the carotid artery as well as in peripheral blood by means of a new, closed, real-time PCR system.\n <\/jats:p>\n \n \n Methods\u2014<\/jats:italic>\n <\/jats:bold>\n Carotid endarterectomy specimens and preoperative peripheral blood mononuclear cells (PBMC) of 75 individuals with severe cerebrovascular atherosclerosis were analyzed by means of a\n C. pneumoniae<\/jats:italic>\n -specific quantitative\n omp<\/jats:italic>\n A-based real-time PCR TaqMan system. Plaques were also cultured onto HEp-2 cells. Before the surgical intervention,\n C. pneumoniae<\/jats:italic>\n -specific IgM, IgG, and IgA as well as C-reactive protein (CRP) levels were determined.\n <\/jats:p>\n \n \n Results\u2014<\/jats:italic>\n <\/jats:bold>\n 89% of all patients studied had\n C. pneumoniae-<\/jats:italic>\n specific antibodies, but the pathogen was not detected in a single carotid atheroma by real-time PCR and cell culture. However,\n C. pneumoniae<\/jats:italic>\n DNA was detected in 4 PBMC samples (5.3%) at very low levels (<1 inclusion\/6 mL EDTA blood). No statistical significance was found between symptomatic\/asymptomatic patients,\n C. pneumoniae<\/jats:italic>\n PCR, results and CRP values after correction for multiplicity-of-test adjustment.\n <\/jats:p>\n \n \n Conclusions\u2014<\/jats:italic>\n <\/jats:bold>\n By means of a closed, highly sensitive, and specific real-time PCR,\n C. pneumoniae<\/jats:italic>\n was not detected in cerebrovascular atherosclerosis. PCR on PBMC was not predictive for endovascular chlamydia infection and most likely stem from previous\n C. pneumoniae<\/jats:italic>\n respiratory tract infection in individual cases.\n <\/jats:p>","DOI":"10.1161\/01.str.0000137765.64705.d8","type":"journal-article","created":{"date-parts":[[2004,7,16]],"date-time":"2004-07-16T00:36:07Z","timestamp":1089938167000},"page":"2024-2028","update-policy":"http:\/\/dx.doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":20,"title":["No Evidence of Involvement of\n Chlamydia pneumoniae<\/i>\n in Severe Cerebrovascular Atherosclerosis by Means of Quantitative Real-Time Polymerase Chain Reaction"],"prefix":"10.1161","volume":"35","author":[{"given":"Petra","family":"Apfalter","sequence":"first","affiliation":[{"name":"From the Department of Clinical Microbiology, Institute of Hygiene and Medical Microbiology, Medical University of Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Barousch","sequence":"additional","affiliation":[{"name":"From the Department of Clinical Microbiology, Institute of Hygiene and Medical Microbiology, Medical University of Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marion","family":"Nehr","sequence":"additional","affiliation":[{"name":"From the Department of Clinical Microbiology, Institute of Hygiene and Medical Microbiology, Medical University of Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgit","family":"Willinger","sequence":"additional","affiliation":[{"name":"From the Department of Clinical Microbiology, Institute of Hygiene and Medical Microbiology, Medical University of Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manfred","family":"Rotter","sequence":"additional","affiliation":[{"name":"From the Department of Clinical Microbiology, Institute of Hygiene and Medical Microbiology, Medical University of Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander M.","family":"Hirschl","sequence":"additional","affiliation":[{"name":"From the Department of Clinical Microbiology, Institute of Hygiene and Medical Microbiology, Medical University of Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.str.0000089015.51603.cc"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000040403.57597.48"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/315596"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/clind\/21.Supplement_3.S244"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00041433-200312000-00009"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-116-4-273"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.2.519-524.2001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.12.4428-4434.2002"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.592-600.2003"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.7.2357-2362.2002"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.7.2622-2627.2000"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960050481"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1454-1457.2003"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.str.0000039322.66575.77"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1067\/mva.2003.200"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/315126"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.12.4408-4411.2000"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1128\/jcm.30.4.796-800.1992","volume":"30","year":"1992","unstructured":"Gaydos CA, Quinn TC, Eiden JJ. Identification of Chlamydia pneumoniae by DNA amplification of the 16S rRNA gene. J Clin Microbiol. 1992; 30: 796\u2013800.","journal-title":"J Clin Microbiol"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/173.4.957"},{"key":"e_1_3_2_20_2","unstructured":"Petrich AK Chong S Boman J Apfalter P Gaydos C Henslee J Kuoppa Y Mannonen L Mygind T Persson K Puolakkainen M Tondella ML Luinstra K Smieja M Mahony J Chernesky M. Multicenter comparison of real time PCR assays and an LCX PCR research test for detection and quantitation of Chlamydia pneumoniae DNA. In: Proceedings of the 43rd Interscience Conference on Antimicrobial Agents and Chemotherapy September 14\u201317 2003. Chicago IL. 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