{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T07:57:00Z","timestamp":1789977420451,"version":"4.0.1"},"reference-count":35,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Background and Purpose\u2014<\/jats:italic>\n            <\/jats:bold>\n            Status epilepticus and cerebral ischemia stimulate persistent neurogenesis in the adult brain, but both conditions cause neuronal damage. We determined whether spreading depression, a common epiphenomenon of these conditions, stimulates persistent neurogenesis.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods\u2014<\/jats:italic>\n            <\/jats:bold>\n            We analyzed the effect of KCl-induced spreading depression on persistent neurogenesis and the spatio-temporal distribution of cells exhibiting immunohistochemical markers for divided and early committed neurons (new neurons) in the adult rat brain.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Results\u2014<\/jats:italic>\n            <\/jats:bold>\n            After induction of spreading depression for 48 hours, the density of mitotic cells, divided cells, and new neurons in the subventricular zone increased at days 1 to 3, days 3 to 6, and day 6, respectively (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.05). The divided cell density in the rostral migratory stream and the stream size increased at day 12 (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.001). Vehicle (saline) infusion or induction of spreading depression for 4 hours only did not increase the divided cell density, but the latter increased new neuron density in the subventricular zone (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.001). Double-labeled new neuron-like cells also appeared in the caudate putamen or cortex in ectopic fashion at day 3, with dramatic increases at days 6 and 12. Administration of the NMDA receptor antagonist, MK-801, which inhibits the propagation of spreading depression, abolished the increase in new neurons in the subventricular zone and the appearance of ectopic new neuron-like cells after 48-hour KCl infusion. There was no neuronal damage, as evidenced by mature neuron density, neurite density, and apoptotic cell appearance after spreading depression for 48 hours.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusions\u2014<\/jats:italic>\n            <\/jats:bold>\n            Spreading depression has the potential to stimulate persistent neurogenesis or to produce ectopic new neuron-like cells.\n          <\/jats:p>","DOI":"10.1161\/01.str.0000169903.09253.c7","type":"journal-article","created":{"date-parts":[[2005,6,3]],"date-time":"2005-06-03T00:50:46Z","timestamp":1117759846000},"page":"1544-1550","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":58,"title":["Induced Spreading Depression Activates Persistent Neurogenesis in the Subventricular Zone, Generating Cells With Markers for Divided and Early Committed Neurons in the Caudate Putamen and Cortex"],"prefix":"10.1161","volume":"36","author":[{"given":"Hiroji","family":"Yanamoto","sequence":"first","affiliation":[{"name":"From the Laboratory for Cerebrovascular Disorders (H.Y., N.T., J.-H.X., Y.N., Y.Nakajo), Research Institute of the National Cardiovascular Center, Department of Cerebrovascular Surgery (H.Y., S.M., I.N.), Hospital of the National Cardiovascular Center (H.K.), Japan; and the Laboratory for Basic Science (Y.Nakajo), Rakuwakai Otowa Hospital, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"From the Laboratory for Cerebrovascular Disorders (H.Y., N.T., J.-H.X., Y.N., Y.Nakajo), Research Institute of the National Cardiovascular Center, Department of Cerebrovascular Surgery (H.Y., S.M., I.N.), Hospital of the National Cardiovascular Center (H.K.), Japan; and the Laboratory for Basic Science (Y.Nakajo), Rakuwakai Otowa Hospital, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norimitsu","family":"Tohnai","sequence":"additional","affiliation":[{"name":"From the Laboratory for Cerebrovascular Disorders (H.Y., N.T., J.-H.X., Y.N., Y.Nakajo), Research Institute of the National Cardiovascular Center, Department of Cerebrovascular Surgery (H.Y., S.M., I.N.), Hospital of the National Cardiovascular Center (H.K.), Japan; and the Laboratory for Basic Science (Y.Nakajo), Rakuwakai Otowa Hospital, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izumi","family":"Nagata","sequence":"additional","affiliation":[{"name":"From the Laboratory for Cerebrovascular Disorders (H.Y., N.T., J.-H.X., Y.N., Y.Nakajo), Research Institute of the National Cardiovascular Center, Department of Cerebrovascular Surgery (H.Y., S.M., I.N.), Hospital of the National Cardiovascular Center (H.K.), Japan; and the Laboratory for Basic Science (Y.Nakajo), Rakuwakai Otowa Hospital, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing-Hui","family":"Xue","sequence":"additional","affiliation":[{"name":"From the Laboratory for Cerebrovascular Disorders (H.Y., N.T., J.-H.X., Y.N., Y.Nakajo), Research Institute of the National Cardiovascular Center, Department of Cerebrovascular Surgery (H.Y., S.M., I.N.), Hospital of the National Cardiovascular Center (H.K.), Japan; and the Laboratory for Basic Science (Y.Nakajo), Rakuwakai Otowa Hospital, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikazu","family":"Nakano","sequence":"additional","affiliation":[{"name":"From the Laboratory for Cerebrovascular Disorders (H.Y., N.T., J.-H.X., Y.N., Y.Nakajo), Research Institute of the National Cardiovascular Center, Department of Cerebrovascular Surgery (H.Y., S.M., I.N.), Hospital of the National Cardiovascular Center (H.K.), Japan; and the Laboratory for Basic Science (Y.Nakajo), Rakuwakai Otowa Hospital, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukako","family":"Nakajo","sequence":"additional","affiliation":[{"name":"From the Laboratory for Cerebrovascular Disorders (H.Y., N.T., J.-H.X., Y.N., Y.Nakajo), Research Institute of the National Cardiovascular Center, Department of Cerebrovascular Surgery (H.Y., S.M., I.N.), Hospital of the National Cardiovascular Center (H.K.), Japan; and the Laboratory for Basic Science (Y.Nakajo), Rakuwakai Otowa Hospital, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruhiko","family":"Kikuchi","sequence":"additional","affiliation":[{"name":"From the Laboratory for Cerebrovascular Disorders (H.Y., N.T., J.-H.X., Y.N., Y.Nakajo), Research Institute of the National Cardiovascular Center, Department of Cerebrovascular Surgery (H.Y., S.M., I.N.), Hospital of the National Cardiovascular Center (H.K.), Japan; and the Laboratory for Basic Science (Y.Nakajo), Rakuwakai Otowa Hospital, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.887941"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901370404"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.5.2074"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-10-03727.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-08-03174.2002"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-19-07768.1998"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)00979-1"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1944.7.6.359"},{"key":"e_1_3_2_9_2","unstructured":"Bure\u0161 J Bure\u0161ov\u00e1 O Kriv\u00e1nek J. 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