{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T22:44:16Z","timestamp":1771627456356,"version":"3.50.1"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[1991,7]]},"abstract":"<jats:p>We used in vivo microdialysis to determine the impact of a focal hypoxic-ischemic insult on striatal amino acid efflux in the immature brain. Microdialysis probes were inserted into the right striatum of postnatal day 7 rats. To induce hypoxic-ischemic injury, the right carotid artery was ligated and the animals were exposed to 8% oxygen for 2.5 hours (n = 22). Rats exposed to ligation alone (n = 10) or hypoxia alone (n = 8) and untreated controls (n = 17) were also studied. Two hours after probe insertion, a 30-minute baseline microdialysis sample was obtained. After arterial ligation, two additional baseline samples were collected. Five more samples were collected over the next 2.5 hours (in 8% oxygen or room air). Eight amino acids (glutamate, aspartate, taurine, glutamine, alanine, serine, glycine, and asparagine) were consistently detected in dialysates using a high-performance liquid chromatography assay with electrochemical detection. In untreated controls, amino acid efflux did not change over 4 hours. During hypoxia-ischemia, efflux values fluctuated widely, with marked intra-animal and interanimal variability. Efflux peaks for each amino acid were defined as values greater than the highest control mean value plus two standard deviations. Glutamate efflux peaks (greater than 7 pmol\/min compared with 2 pmol\/min at baseline) were detected in no controls and in eight hypoxic-ischemic rats (p = 0.006, Fisher's two-tailed exact test). Taurine efflux peaks (greater than 75 pmol\/min compared with 10 pmol\/min for controls at baseline) were detected in 10 hypoxic-ischemic rats and one control (p = 0.01) and in seven of the eight animals in which glutamate efflux peaks occurred (p = 0.006).(ABSTRACT TRUNCATED AT 250 WORDS)<\/jats:p>","DOI":"10.1161\/01.str.22.7.928","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T20:08:03Z","timestamp":1308341283000},"page":"928-932","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":42,"title":["Effects of perinatal stroke on striatal amino acid efflux in rats studied with in vivo microdialysis."],"prefix":"10.1161","volume":"22","author":[{"given":"K E","family":"Gordon","sequence":"first","affiliation":[{"name":"Department of Pediatrics, University of Michigan, Ann Arbor 48109-0570."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Simpson","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Michigan, Ann Arbor 48109-0570."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Statman","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Michigan, Ann Arbor 48109-0570."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F S","family":"Silverstein","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Michigan, Ann Arbor 48109-0570."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Stroke"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.STR.22.7.928","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T22:13:34Z","timestamp":1715552014000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.STR.22.7.928"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,7]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1991,7]]}},"alternative-id":["10.1161\/01.STR.22.7.928"],"URL":"https:\/\/doi.org\/10.1161\/01.str.22.7.928","relation":{},"ISSN":["0039-2499","1524-4628"],"issn-type":[{"value":"0039-2499","type":"print"},{"value":"1524-4628","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,7]]},"assertion":[{"value":"1991-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}