{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:16:42Z","timestamp":1780532202092,"version":"3.54.1"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[1992,9]]},"abstract":"<jats:p>The N-methyl-D-aspartate receptor is believed to mediate part of the ischemic neuronal damage caused by the excitatory amino acid glutamate. (+)SKF 10,047, the prototypic sigma-agonist, interacts with the N-methyl-D-aspartate receptor. Therefore, we studied the neuroprotective effect of (+)SKF 10,047 on cultured rat cerebellar neurons and on CA1 hippocampal neurons of gerbils exposed to brain ischemia.<\/jats:p>\n          <jats:p>Mechanisms of neuroprotection were studied in vitro by measuring calcium influx into cultured rat cerebellar granule cells loaded with fura 2-AM. In vivo neuroprotection of gerbil CA1 hippocampal neurons was studied in a posttreatment regimen following 5 minutes of bilateral carotid artery occlusion and 7 days of reperfusion.<\/jats:p>\n          <jats:p>In primary cultured rat cerebellar granule cell neurons, (+)SKF 10,047 in a dose-dependent manner diminished intracellular calcium levels of N-methyl-D-aspartate-stimulated neurons by a maximum of 87% (n = 8), with a 50% inhibitory concentration of 0.8 microM. (+)SKF 10,047 did not prevent subsequent calcium influx stimulated by kainic acid or KCl, nor did it interfere with modulation of the kainate response by quisqualic acid. Neuroprotection of 64% (p = 0.006, n = 15) of gerbil CA1 hippocampal neurons was achieved by posttreatment injection followed by minipump infusion.<\/jats:p>\n          <jats:p>Neuroprotection by (+)SKF 10,047 most likely involves interaction at the N-methyl-D-aspartate receptor. These results suggest that the benzomorphan class of sigma-agonists may provide neuroprotection in cerebral ischemia and stroke.<\/jats:p>","DOI":"10.1161\/01.str.23.9.1319","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T20:08:03Z","timestamp":1308341283000},"page":"1319-1323","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":18,"title":["Neuroprotective mechanism of (+)SKF 10,047 in vitro and in gerbil global brain ischemia."],"prefix":"10.1161","volume":"23","author":[{"given":"P G","family":"Lysko","sequence":"first","affiliation":[{"name":"Department of Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pa. 19406-0939."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T L","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pa. 19406-0939."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J L","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pa. 19406-0939."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Feuerstein","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pa. 19406-0939."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","container-title":["Stroke"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.STR.23.9.1319","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T22:34:00Z","timestamp":1715553240000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.STR.23.9.1319"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,9]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1992,9]]}},"alternative-id":["10.1161\/01.STR.23.9.1319"],"URL":"https:\/\/doi.org\/10.1161\/01.str.23.9.1319","relation":{},"ISSN":["0039-2499","1524-4628"],"issn-type":[{"value":"0039-2499","type":"print"},{"value":"1524-4628","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,9]]},"assertion":[{"value":"1992-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}