{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T12:21:21Z","timestamp":1767183681163},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[1994,8]]},"abstract":"<jats:p>Nitric oxide-mediated cerebral vasodilation is altered in spontaneously hypertensive stroke-prone rats. Stroke predisposition in this strain could be related to a genetic defect of brain nitric oxide synthase, the enzyme responsible for nitric oxide production. We tested the hypothesis that brain nitric oxide synthase activity is altered in spontaneously hypertensive stroke-prone rats compared with spontaneously hypertensive or Wistar-Kyoto rats.<\/jats:p>\n          <jats:p>A colony of spontaneously hypertensive stroke-prone rats was bred, in which the rate of neurological events under salt load was assessed. In a separate cohort of animals brain nitric oxide synthase activity was measured in spontaneously hypertensive stroke-prone rats (n = 6) and in spontaneously hypertensive (n = 6) and genetically related Wistar-Kyoto rats (n = 6). Calcium dependency of nitric oxide synthase was also assessed in cortical brain samples from the three rat strains to determine if altered calcium-dependent activation of nitric oxide synthase was present.<\/jats:p>\n          <jats:p>Brain nitric oxide synthase activity was highest in the cerebellum (eg, spontaneously hypertensive stroke-prone rats: cerebral cortex, 10.6 +\/- 0.9; cerebellum, 50.1 +\/- 12.0; brain stem, 14.7 +\/- 10.3 pmol\/mg protein per minute); however, there was no difference among the three rat strains in any region (eg, cerebral cortex: spontaneously hypertensive stroke-prone, 10.6 +\/- 0.9; spontaneously hypertensive, 10.8 +\/- 0.5; Wistar-Kyoto, 10.9 +\/- 0.7 pmol\/mg protein per minute) or at any calcium concentration tested.<\/jats:p>\n          <jats:p>A genetic defect of brain nitric oxide synthase is unlikely to be the cause of stroke predisposition in spontaneously hypertensive stroke-prone rats.<\/jats:p>","DOI":"10.1161\/01.str.25.8.1674","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T20:08:03Z","timestamp":1308341283000},"page":"1674-1677","update-policy":"http:\/\/dx.doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":24,"title":["Brain nitric oxide synthase activity in normal, hypertensive, and stroke-prone rats."],"prefix":"10.1161","volume":"25","author":[{"given":"N","family":"Clavier","sequence":"first","affiliation":[{"name":"Department of Anesthesiology and Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore, Md."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J R","family":"Tobin","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore, Md."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J R","family":"Kirsch","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore, Md."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Izuta","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore, Md."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R J","family":"Traystman","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore, Md."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Stroke"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.STR.25.8.1674","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T22:02:54Z","timestamp":1715551374000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.STR.25.8.1674"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,8]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1994,8]]}},"alternative-id":["10.1161\/01.STR.25.8.1674"],"URL":"https:\/\/doi.org\/10.1161\/01.str.25.8.1674","relation":{},"ISSN":["0039-2499","1524-4628"],"issn-type":[{"value":"0039-2499","type":"print"},{"value":"1524-4628","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,8]]},"assertion":[{"value":"1994-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}