{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T09:35:16Z","timestamp":1768901716806,"version":"3.49.0"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[1998,10]]},"abstract":"<jats:p><jats:italic>Background and Purpose<\/jats:italic>\u2014Subarachnoid hemorrhage (SAH) impairs both contraction and relaxation response in cerebral arteries. We tested the hypothesis that cerebral vasospasm might be ATP-independent contraction, such as latch state, and protein synthesis might be substantially downregulated due to ATP consumption after long-lasting contraction.<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>\u2014Chronic cerebral vasospasm was induced in the canine 2-hemorrhage model of SAH. The normal and spastic basilar arteries were stabilized in Krebs-Henseleit solution, and contraction was induced by 30 \u03bcmol\/L prostaglandin F<jats:sub>2\u03b1<\/jats:sub>(PGF<jats:sub>2\u03b1<\/jats:sub>) in vitro and in vivo. Before and at 15 minutes and 1 hour after the treatment with PGF<jats:sub>2\u03b1<\/jats:sub>, the levels of phosphorylated 20-kDa myosin light chain (MLC<jats:sub>20<\/jats:sub>) were measured. The time course of expression of contraction proteins actin and MLC<jats:sub>20<\/jats:sub>, and contraction-inhibiting proteins<jats:italic>h<\/jats:italic>-caldesmon and calponin was determined by immunoblotting techniques.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>\u2014A significant vasospasm occurred in the basilar artery during days 4 to 21, most prominently on days 7 and 14. There were no significant differences in the baseline levels of phosphorylated MLC<jats:sub>20<\/jats:sub>between normal and spastic basilar arteries. The increase in MLC<jats:sub>20<\/jats:sub>phosphorylation by PGF<jats:sub>2\u03b1<\/jats:sub>was significantly attenuated in the spastic basilar artery in vitro and in vivo (<jats:italic>P<\/jats:italic>&lt;0.05). The immunoreactivity for actin,<jats:italic>h<\/jats:italic>-caldesmon, and calponin in the spastic basilar arteries was progressively decreased until day 14 and returned to the normal level on day 21. In contrast, protein levels of MLC<jats:sub>20<\/jats:sub>did not significantly change during days 0 to 21.<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>\u2014Chronic cerebral vasospasm closely resembles the latch state, and temporary deficiencies of contractile proteins may result from increased destruction and inhibition of protein synthesis.<\/jats:p>","DOI":"10.1161\/01.str.29.10.2149","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T20:08:03Z","timestamp":1308341283000},"page":"2149-2154","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":24,"title":["Myosin Light Chain Phosphorylation and Contractile Proteins in a Canine Two-Hemorrhage Model of Subarachnoid Hemorrhage"],"prefix":"10.1161","volume":"29","author":[{"given":"Hong","family":"Sun","sequence":"first","affiliation":[{"name":"From the Department of Neurosurgery (H.Sun, K.K., H.Suzuki, T.K., S.W.) and First Department of Internal Medicine (M.I., Y.K., T.N.), Mie University School of Medicine, Mie, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Kanamaru","sequence":"additional","affiliation":[{"name":"From the Department of Neurosurgery (H.Sun, K.K., H.Suzuki, T.K., S.W.) and First Department of Internal Medicine (M.I., Y.K., T.N.), Mie University School of Medicine, Mie, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaaki","family":"Ito","sequence":"additional","affiliation":[{"name":"From the Department of Neurosurgery (H.Sun, K.K., H.Suzuki, T.K., S.W.) and First Department of Internal Medicine (M.I., Y.K., T.N.), Mie University School of Medicine, Mie, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidenori","family":"Suzuki","sequence":"additional","affiliation":[{"name":"From the Department of Neurosurgery (H.Sun, K.K., H.Suzuki, T.K., S.W.) and First Department of Internal Medicine (M.I., Y.K., T.N.), Mie University School of Medicine, Mie, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadashi","family":"Kojima","sequence":"additional","affiliation":[{"name":"From the Department of Neurosurgery (H.Sun, K.K., H.Suzuki, T.K., S.W.) and First Department of Internal Medicine (M.I., Y.K., T.N.), Mie University School of Medicine, Mie, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiro","family":"Waga","sequence":"additional","affiliation":[{"name":"From the Department of Neurosurgery (H.Sun, K.K., H.Suzuki, T.K., S.W.) and First Department of Internal Medicine (M.I., Y.K., T.N.), Mie University School of Medicine, Mie, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuko","family":"Kureishi","sequence":"additional","affiliation":[{"name":"From the Department of Neurosurgery (H.Sun, K.K., H.Suzuki, T.K., S.W.) and First Department of Internal Medicine (M.I., Y.K., T.N.), Mie University School of Medicine, Mie, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Nakano","sequence":"additional","affiliation":[{"name":"From the Department of Neurosurgery (H.Sun, K.K., H.Suzuki, T.K., S.W.) and First Department of Internal Medicine (M.I., Y.K., T.N.), Mie University School of Medicine, Mie, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1991.17"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1992.76.4.0635"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1993.30"},{"key":"e_1_3_2_4_2","first-page":"711","volume":"31","year":"1992","unstructured":"Nishizawa S, Peterson JW, Shimoyama I, Uemura K. 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