{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:32:58Z","timestamp":1762101178618,"version":"3.38.0"},"reference-count":53,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[1999,11]]},"abstract":"<jats:p><jats:italic>Background and Purpose<\/jats:italic>\u2014Cerebral arteries develop stretch-induced myogenic tone, which plays an important role in the regulation of blood flow to the brain. Although the effect of oxidized LDL (Ox-LDL) on many aspects of the vascular endothelial and smooth muscle cell function have been extensively investigated, its influence on myogenic activity has not been studied.<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>\u2014The effect of Ox-LDL on the myogenic tone that develops in the perfused rabbit posterior cerebral artery at intramural pressures between 40 and 90 mm Hg was examined.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>\u2014Ox-LDL (10 \u03bcg\/mL) significantly enhanced myogenic tone by 21.4\u00b16.1% to 28.5\u00b11.8% at 60 to 90 mm Hg pressure (<jats:italic>P<\/jats:italic>&lt;0.05) but had no influence on norepinephrine- (0.5 to 1 \u03bcmol\/L) and KCl (20 mmol\/L)\u2013induced constriction. Ox-LDL was effective whether the artery was exposed to it from the intraluminal or the extraluminal surface. Lysophosphatidylcholine (10 \u03bcmol\/L), a lipid component of Ox-LDL, had an equivalent potentiating effect. Native LDL (100 \u03bcg\/mL) was inactive. The myogenic tone\u2013potentiating effect of Ox-LDL was abolished by endothelium removal but was not influenced by the NO synthase inhibitor<jats:italic>N<\/jats:italic><jats:sup>G<\/jats:sup>-nitro-<jats:sc>l<\/jats:sc>-nitro-arginine methyl ester (50 \u03bcmol\/L). This effect was reversed by the endothelin-1 (ET-1) antagonist BQ-123 (1 \u03bcmol\/L). This concentration blocked 1 to 3 nmol\/L ET-1\u2013induced constriction without altering constriction induced by 40 mmol\/L KCl. The potentiating effect was suppressed by the specific protein kinase C inhibitor chelerythrine (1 \u03bcmol\/L).<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>\u2014Ox-LDL enhances myogenic tone through the release of ET-1 from the endothelium of the rabbit posterior cerebral artery.<\/jats:p>","DOI":"10.1161\/01.str.30.11.2423","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T20:08:03Z","timestamp":1308341283000},"page":"2423-2430","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":11,"title":["Oxidized Low-Density Lipoprotein Enhances Myogenic Tone in the Rabbit Posterior Cerebral Artery Through the Release of Endothelin-1"],"prefix":"10.1161","volume":"30","author":[{"given":"Hui","family":"Xie","sequence":"first","affiliation":[{"name":"From the Totman Laboratory for Cerebrovascular Research, Department of Pharmacology, College of Medicine, University of Vermont, Burlington."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John A.","family":"Bevan","sequence":"additional","affiliation":[{"name":"From the Totman Laboratory for Cerebrovascular Research, Department of Pharmacology, College of Medicine, University of Vermont, Burlington."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/362801a0"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198904063201407"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114271"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.12.3883"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(90)90142-6"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.6.5.505"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1990.tb12045.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/S0022-3565(24)36994-0","volume":"283","year":"1997","unstructured":"Cox DA, Cohen ML. 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