{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T23:08:38Z","timestamp":1789945718588,"version":"4.0.1"},"reference-count":54,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[1999,5]]},"abstract":"<jats:p>\n            <jats:italic>Background and Purpose<\/jats:italic>\n            \u2014Apolipoprotein E (apoE) has been found relevant in a variety of central nervous system disorders. This experiment examined the effect of endogenous murine apoE on selective neuronal necrosis resulting from a transient forebrain ischemia insult.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Methods<\/jats:italic>\n            \u2014ApoE deficient (n=16) and wild type (n=17) halothane-anesthetized mice were subjected to severe forebrain ischemia (10 minutes of bilateral carotid occlusion and systemic hypotension). After 3 days\u2019 recovery, brain injury was determined histologically. In other apoE-deficient and wild-type mice, regional cerebral blood flow (CBF) was determined by\n            <jats:sup>14<\/jats:sup>\n            C-iodoantipyrine autoradiography 10 minutes before, 5 minutes after onset of, and 30 minutes after reperfusion from 10 minutes of forebrain ischemia.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Results<\/jats:italic>\n            \u2014The percentage of dead hippocampal CA1 neurons (mean\u00b1SD) was greater in the apoE-deficient group (apoE deficient=67\u00b130%; wild type=37\u00b133%;\n            <jats:italic>P<\/jats:italic>\n            =0.011). A similar pattern was observed in the caudoputamen (\n            <jats:italic>P<\/jats:italic>\n            =0.002) and neocortex (\n            <jats:italic>P<\/jats:italic>\n            =0.014). Cerebral blood flow was similar between groups at each measurement interval. Marked hypoperfusion persisted in both groups at 30 minutes after ischemia.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Conclusions<\/jats:italic>\n            \u2014ApoE deficiency worsens ischemic outcome. This is not attributable to effects on CBF. A role of apoE in the cerebral response to global ischemia is consistent with prior reports that murine apoE deficiency increases infarct size resulting from focal cerebral ischemia.\n          <\/jats:p>","DOI":"10.1161\/01.str.30.5.1118","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T20:08:03Z","timestamp":1308341283000},"page":"1118-1124","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":100,"title":["Apolipoprotein E Deficiency Worsens Outcome From Global Cerebral Ischemia in the Mouse"],"prefix":"10.1161","volume":"30","author":[{"given":"Huaxin","family":"Sheng","sequence":"first","affiliation":[{"name":"From the Departments of Anesthesiology (H.S., D.S.W.), Medicine (Neurology) (D.T.L.), and Surgery (R.D.P., D.S.W.), Duke University Medical Center, Durham, NC; Department of Anesthesiology, Klinikum rechts der Isar, Technische Universitat Munchen, Munich, Germany (G.B.M.); and the Department of Anesthesiology and Resuscitation, Yamagata University, Yamagata, Japan (M.K.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel T.","family":"Laskowitz","sequence":"additional","affiliation":[{"name":"From the Departments of Anesthesiology (H.S., D.S.W.), Medicine (Neurology) (D.T.L.), and Surgery (R.D.P., D.S.W.), Duke University Medical Center, Durham, NC; Department of Anesthesiology, Klinikum rechts der Isar, Technische Universitat Munchen, Munich, Germany (G.B.M.); and the Department of Anesthesiology and Resuscitation, Yamagata University, Yamagata, Japan (M.K.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Burkhard","family":"Mackensen","sequence":"additional","affiliation":[{"name":"From the Departments of Anesthesiology (H.S., D.S.W.), Medicine (Neurology) (D.T.L.), and Surgery (R.D.P., D.S.W.), Duke University Medical Center, Durham, NC; Department of Anesthesiology, Klinikum rechts der Isar, Technische Universitat Munchen, Munich, Germany (G.B.M.); and the Department of Anesthesiology and Resuscitation, Yamagata University, Yamagata, Japan (M.K.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaya","family":"Kudo","sequence":"additional","affiliation":[{"name":"From the Departments of Anesthesiology (H.S., D.S.W.), Medicine (Neurology) (D.T.L.), and Surgery (R.D.P., D.S.W.), Duke University Medical Center, Durham, NC; Department of Anesthesiology, Klinikum rechts der Isar, Technische Universitat Munchen, Munich, Germany (G.B.M.); and the Department of Anesthesiology and Resuscitation, Yamagata University, Yamagata, Japan (M.K.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert D.","family":"Pearlstein","sequence":"additional","affiliation":[{"name":"From the Departments of Anesthesiology (H.S., D.S.W.), Medicine (Neurology) (D.T.L.), and Surgery (R.D.P., D.S.W.), Duke University Medical Center, Durham, NC; Department of Anesthesiology, Klinikum rechts der Isar, Technische Universitat Munchen, Munich, Germany (G.B.M.); and the Department of Anesthesiology and Resuscitation, Yamagata University, Yamagata, Japan (M.K.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Warner","sequence":"additional","affiliation":[{"name":"From the Departments of Anesthesiology (H.S., D.S.W.), Medicine (Neurology) (D.T.L.), and Surgery (R.D.P., D.S.W.), Duke University Medical Center, Durham, NC; Department of Anesthesiology, Klinikum rechts der Isar, Technische Universitat Munchen, Munich, Germany (G.B.M.); and the Department of Anesthesiology and Resuscitation, Yamagata University, Yamagata, Japan (M.K.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3233(08)60642-7"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.13.4169"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(86)90519-7"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440020306"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)91411-0"},{"key":"e_1_3_2_6_2","first-page":"818","volume":"227","year":"1997","unstructured":"Slooter AJ, Tang MX, van Duijn CM, Stern Y, Ott A, Bell K, Breteler MM, Van Broeckhoven C, Tatemichi TK, Tycko B, Hofman A, Mayeux R. Apolipoprotein E \u03b54 and risk of dementia with stroke, a population-based investigation. JAMA. 1997;227:818\u2013821.","journal-title":"JAMA"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0295-135"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4975(97)00757-1"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)04318-3"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1997.03550020068040"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.29.9.1882"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199707000-00005"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199808030-00035"},{"key":"e_1_3_2_14_2","first-page":"774","volume":"24","year":"1998","unstructured":"Sheng H, Laskowitz DT, Pearlstein RD, Warner DS: Characterization of a recovery global cerebral ischemia model in the mouse. Soc Neurosci Abs. 1998;24:774.15.","journal-title":"Soc Neurosci Abs"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.10.4471"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0404.1984.tb07822.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.18.2.3564110"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.27.9.1578"},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","unstructured":"Kaplan HM Brewer NR. Physiology. In: Kaplan HM Brewer NR Blair WH eds. The Mouse in Biomedical Research. Vol 3. San Diego CA: Academic Press; 1983;251.","DOI":"10.1016\/B978-0-12-262503-9.50018-9"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1978.234.1.H59"},{"key":"e_1_3_2_21_2","unstructured":"Franklin BJ Paxinos G. The Mouse Brain in Stereotactic Coordinates. San Diego Calif: Academic Press; 1997."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199804000-00003"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/nbdi.1996.0023"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6795720"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90362-G"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1161\/atv91.14.1.8274470"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1411543"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1995.1088"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(95)12006-P"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199707280-00018"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)01420-5"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.136.10.3716"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(97)00021-0"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0996-55"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1995.1062"},{"key":"e_1_3_2_36_2","first-page":"343","volume":"22","year":"1996","unstructured":"Horsburgh K, Nicoll J. Selective alterations in the cellular distribution of apolipoprotein E immunoreactivity following transient global ischaemia in the rat. Neuropathol App Neurobiol. 1996;22:343\u2013349.","journal-title":"Neuropathol App Neurobiol"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(94)01467-V"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-20-08292.1998"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(98)00208-5"},{"key":"e_1_3_2_40_2","first-page":"482","volume":"24","year":"1998","unstructured":"Sheng H, Brody T, Pearlstein RD, Crapo J, Warner DS. Extracellular superoxide dismutase deficient mice have decreased resistance to focal cerebral ischemia. Soc Neurosci Abs. 1998;24:482.14.","journal-title":"Soc Neurosci Abs"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.11.5219"},{"key":"e_1_3_2_42_2","first-page":"617","volume":"75","year":"1996","unstructured":"Oury TD, Day BJ, Crapo JD. Extracellular superoxide dismutase: a regulator of nitric oxide bioavailability. Lab Invest. 1996;75:617\u2013636.","journal-title":"Lab Invest"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.28.9.1805"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)01217-1"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)01453-9"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.28.4.837"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.23.8085"},{"key":"e_1_4_3_1_1_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199805000-00001"},{"key":"e_1_4_3_1_2_2","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1046\/j.1471-4159.1999.0721069.x","volume":"72","year":"1999","unstructured":"Moulder KL, Narita M, Chang LK, Bu G, Johnson EM Jr. Analysis of a novel mechanism of neuronal toxicity produced by an apolipoprotein E-derived peptide. J Neurochem. 1999;72:1069\u20131080.","journal-title":"J Neurochem"},{"key":"e_1_4_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-15-05678.1997"},{"key":"e_1_4_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-01-00195.1998"},{"key":"e_1_4_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0694-180"},{"key":"e_1_4_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8346443"},{"key":"e_1_4_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.23.8085"}],"container-title":["Stroke"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.STR.30.5.1118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T22:20:32Z","timestamp":1715552432000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.STR.30.5.1118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,5]]},"references-count":54,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1999,5]]}},"alternative-id":["10.1161\/01.STR.30.5.1118"],"URL":"https:\/\/doi.org\/10.1161\/01.str.30.5.1118","relation":{},"ISSN":["0039-2499","1524-4628"],"issn-type":[{"value":"0039-2499","type":"print"},{"value":"1524-4628","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,5]]},"assertion":[{"value":"1998-10-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-02-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}