{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:32:53Z","timestamp":1762101173569,"version":"3.38.0"},"reference-count":53,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[1999,8]]},"abstract":"<jats:p><jats:italic>Background and Purpose<\/jats:italic>\u2014Rat major cerebral arteries seem to receive serotonergic fibers originating from the dorsal raphe nucleus (DRN), but little is known about their function. The aim of our present work was to establish a functional relationship between this brain stem nucleus and the cerebral blood vessels by studying the effects of several treatments in the DRN on cerebrovascular serotonergic activity.<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>\u2014Serotonin, clomipramine, 8-OH-DPAT, and WAY-100635 were administered in DRN. A stereotaxically localized electrode allowed the electrical stimulation of this brain stem nucleus. Serotonergic activity was appraised in major cerebral arteries, striatum, and hippocampus from 5-hydroxytryptophan accumulation after aromatic<jats:sc>l<\/jats:sc>-amino acid decarboxylase inhibition with NSD-1015.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>\u2014Serotonin significantly decreased serotonergic activity in major cerebral arteries and striatum without affecting it in hippocampus. This reduction was blocked by previous injection of WAY-100635 in DRN. Local administration of 8-OH-DPAT or clomipramine elicited an effect similar to that of serotonin, whereas that of WAY-100635 did not modify serotonergic activity in either of the tissues. Electrical stimulation of DRN significantly increased serotonergic activity in major cerebral arteries and striatum but not in hippocampus.<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>\u2014These results confirm the presence of a serotonergic innervation in rat major cerebral arteries functionally related to DRN. 5-HT<jats:sub>1A<\/jats:sub>receptor activation partly mediates the action of serotonin in DRN. A serotonergic tone acting on these somatodendritic receptors was not clearly found.<\/jats:p>","DOI":"10.1161\/01.str.30.8.1695","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T20:10:52Z","timestamp":1308341452000},"page":"1695-1701","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":9,"title":["Local Treatments of Dorsal Raphe Nucleus Induce Changes in Serotonergic Activity in Rat Major Cerebral Arteries"],"prefix":"10.1161","volume":"30","author":[{"given":"Emilio J.","family":"Marco","sequence":"first","affiliation":[{"name":"From the Departamento de Fisiologi\u0301a, Facultad de Medicina, Universidad Auto\u0301noma de Madrid, Spain."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari\u0301a Jesu\u0301s","family":"Moreno","sequence":"additional","affiliation":[{"name":"From the Departamento de Fisiologi\u0301a, Facultad de Medicina, Universidad Auto\u0301noma de Madrid, Spain."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angel Luis Lo\u0301pez","family":"de Pablo","sequence":"additional","affiliation":[{"name":"From the Departamento de Fisiologi\u0301a, Facultad de Medicina, Universidad Auto\u0301noma de Madrid, Spain."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","first-page":"85","volume":"206","year":"1978","unstructured":"Reinhard JF Jr, Leibmann J, Schlosberg A, Moskowitz MA. Serotonin neurons project to small blood vessels in the brain. Science. 1978;206:85\u201387.","journal-title":"Science"},{"doi-asserted-by":"publisher","key":"e_1_3_2_2_2","DOI":"10.1016\/0006-8993(82)91266-5"},{"key":"e_1_3_2_3_2","first-page":"561","volume":"1","year":"1983","unstructured":"Griffith SG, Burnstock G. Immunohistochemical demonstration of serotonin in nerves supplying human cerebral and mesenteric blood vessels. Lancet. 1983;1:561\u2013562.","journal-title":"Lancet"},{"doi-asserted-by":"publisher","key":"e_1_3_2_4_2","DOI":"10.1038\/306055a0"},{"doi-asserted-by":"publisher","key":"e_1_3_2_5_2","DOI":"10.1016\/0006-8993(85)90997-7"},{"doi-asserted-by":"publisher","key":"e_1_3_2_6_2","DOI":"10.1159\/000411600"},{"doi-asserted-by":"publisher","key":"e_1_3_2_7_2","DOI":"10.1016\/0165-0173(91)90009-W"},{"doi-asserted-by":"publisher","key":"e_1_3_2_8_2","DOI":"10.1007\/BF00501930"},{"doi-asserted-by":"publisher","key":"e_1_3_2_9_2","DOI":"10.1111\/j.1748-1716.1984.tb07459.x"},{"doi-asserted-by":"publisher","key":"e_1_3_2_10_2","DOI":"10.1016\/0006-8993(86)91218-7"},{"doi-asserted-by":"publisher","key":"e_1_3_2_11_2","DOI":"10.1161\/res.60.2.3552285"},{"doi-asserted-by":"publisher","key":"e_1_3_2_12_2","DOI":"10.1016\/0006-8993(88)91608-3"},{"doi-asserted-by":"publisher","key":"e_1_3_2_13_2","DOI":"10.1016\/0306-4522(89)90072-9"},{"key":"e_1_3_2_14_2","first-page":"33","volume":"26","year":"1989","unstructured":"Yu J-G, Lee TJ-F. 5-Hydroxytryptamine-containing fibers in cerebral arteries of the cat, rat and guinea-pig. Blood Vessels. 1989;26:33\u201343.","journal-title":"Blood Vessels"},{"doi-asserted-by":"publisher","key":"e_1_3_2_15_2","DOI":"10.1016\/0006-8993(92)90184-B"},{"doi-asserted-by":"publisher","key":"e_1_3_2_16_2","DOI":"10.1177\/37.9.2671153"},{"doi-asserted-by":"publisher","key":"e_1_3_2_17_2","DOI":"10.1016\/0304-3940(82)90208-7"},{"key":"e_1_3_2_18_2","first-page":"463","volume":"16","year":"1985","unstructured":"Tsai S-H, Lin S-Z, Wang S-D, Liu J-C, Shih C-J. Retrograde localization of the innervation of the middle cerebral artery with horseradish peroxidase in cats. Neurosurgery. 1985;16:463\u2013467.","journal-title":"Neurosurgery"},{"doi-asserted-by":"publisher","key":"e_1_3_2_19_2","DOI":"10.1016\/0006-8993(85)90255-0"},{"doi-asserted-by":"publisher","key":"e_1_3_2_20_2","DOI":"10.1161\/str.26.2.271"},{"doi-asserted-by":"publisher","key":"e_1_3_2_21_2","DOI":"10.1016\/0014-2999(85)90494-7"},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"2060","DOI":"10.1046\/j.1471-4159.1996.67052060.x","volume":"67","year":"1996","unstructured":"L\u00f3pez de Pablo AL, Moreno MJ, Marco EJ. In vivo tryptophan hydroxylase activity in rat major cerebral arteries is decreased by dorsal raphe nucleus lesions. J Neurochem. 1996;67:2060\u20132065.","journal-title":"J Neurochem"},{"doi-asserted-by":"publisher","key":"e_1_3_2_23_2","DOI":"10.1161\/str.25.5.8165676"},{"doi-asserted-by":"publisher","key":"e_1_3_2_24_2","DOI":"10.1016\/S0006-8993(96)01382-0"},{"doi-asserted-by":"publisher","key":"e_1_3_2_25_2","DOI":"10.3171\/jns.1974.40.4.0433"},{"key":"e_1_3_2_26_2","first-page":"53","volume":"12","year":"1975","unstructured":"Urquilla PR, Marco EJ, Lluch S. Pharmacological receptors of cerebral arteries of the goat. Blood Vessels. 1975;12:53\u201367.","journal-title":"Blood Vessels"},{"doi-asserted-by":"publisher","key":"e_1_3_2_27_2","DOI":"10.1161\/res.42.1.618595"},{"doi-asserted-by":"publisher","key":"e_1_3_2_28_2","DOI":"10.1016\/0006-8993(91)90448-5"},{"key":"e_1_3_2_29_2","first-page":"91","volume":"1","year":"1969","unstructured":"Neff NH, Lin RC, Ngai SH, Costa E. Turnover rate measurements of brain serotonin in unanesthetized rats. Adv Biochem Psychopharmacol.. 1969;1:91\u2013109.","journal-title":". Adv Biochem Psychopharmacol."},{"doi-asserted-by":"publisher","key":"e_1_3_2_30_2","DOI":"10.1111\/j.2042-7158.1988.tb05215.x"},{"key":"e_1_3_2_31_2","first-page":"175","volume":"50","year":"1994","unstructured":"Moreno MJ, L\u00f3pez de Pablo AL, Conde MV, Fraile ML, Marco EJ. Serotonergic activity in rat cerebral arteries depends on dorsal raphe nucleus but not on median raphe nucleus. Rev Esp Fisiol.. 1994;50:175\u2013182.","journal-title":". Rev Esp Fisiol."},{"unstructured":"Letner C. Buffer solutions. In: Letner C ed. Geigy Scientific Tables. 3rd ed. Basel Switzerland: Ciba-Geigy Ltd; 1984;3:58\u201360.","key":"e_1_3_2_32_2"},{"unstructured":"K\u00f6nig JFR Klippel RA. The Rat Brain. Huntington NY: Robert E. Krieger Publishing Co Inc; 1974.","key":"e_1_3_2_33_2"},{"doi-asserted-by":"publisher","key":"e_1_3_2_34_2","DOI":"10.1016\/0006-8993(76)90050-0"},{"unstructured":"Steinbusch HWM. Serotonin-immunoreactive neurons and their projections in the CNS. In: Bj\u00f6rklund A H\u00f6kfelt T Kuhar MJ eds. Handbook of Chemical Neuroanatomy. Vol. 3: Classical Transmitters and Transmitter Receptors in the CNS Part II. Amsterdam Netherlands: Elsevier Science Publishers BV; 1984:68\u2013125.","key":"e_1_3_2_35_2"},{"doi-asserted-by":"publisher","key":"e_1_3_2_36_2","DOI":"10.1016\/0028-3908(85)90095-4"},{"key":"e_1_3_2_37_2","first-page":"354","volume":"193","year":"1951","unstructured":"Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin phenol reagent. J Biol Chem. 1951;193:354\u2013357.","journal-title":"J Biol Chem"},{"doi-asserted-by":"publisher","key":"e_1_3_2_38_2","DOI":"10.1016\/0014-2999(81)90481-7"},{"doi-asserted-by":"crossref","unstructured":"Theodorson-Norheim E. Kruskal-Wallis test: BASIC computer program to perform nonparametric one-way analysis of variance and multiple comparisons on ranks of several independents samples. Comput Methods Programs Biomed. 186;23:57\u201362.","key":"e_1_3_2_39_2","DOI":"10.1016\/0169-2607(86)90081-7"},{"doi-asserted-by":"publisher","key":"e_1_3_2_40_2","DOI":"10.1097\/00004714-198712001-00001"},{"doi-asserted-by":"publisher","key":"e_1_3_2_41_2","DOI":"10.1016\/0306-4522(90)90248-3"},{"key":"e_1_3_2_42_2","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1016\/S0022-3565(25)38872-5","volume":"269","year":"1994","unstructured":"Kreiss DS, Lucki I. Differential regulation of serotonin (5-HT) release in the striatum and hippocampus by 5-HT1A autoreceptors of the dorsal and median raphe nuclei. J Pharmacol Exp Ther. 1994;269:1268\u20131279.","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_3_2_43_2","first-page":"237","volume":"343","year":"1991","unstructured":"Adell A, Artigas F. Differential effects of clomipramine given locally or systemically on extracellular 5-hydroxytryptamine in raphe nuclei and frontal cortex. Naunyn Schmiedebergs Arch Pharmacol. 1991;343:237\u2013244.","journal-title":"Naunyn Schmiedebergs Arch Pharmacol"},{"key":"e_1_3_2_44_2","first-page":"101","volume":"232","year":"1993","unstructured":"Bel N, Artigas F. Fluvoxamine preferentially increases extracellular 5-hydroxytryptamine in the raphe nuclei: an in vivo microdialysis study. Eur J Pharmacol.. 1993;232:101\u2013103.","journal-title":". Eur J Pharmacol."},{"doi-asserted-by":"publisher","key":"e_1_3_2_45_2","DOI":"10.1016\/0014-2999(95)00573-4"},{"doi-asserted-by":"crossref","unstructured":"Jacobs BL Fornal CA. Physiology and pharmacology of brain serotonergic neurons. In: Baumgarten HG G\u00f6thert M eds. Handbook of Experimental Pharmacology: Serotoninergic Neurons and 5-HT Receptors in CNS. Berlin Germany: Springer; 1997:91\u201396.","key":"e_1_3_2_46_2","DOI":"10.1007\/978-3-642-60921-3_3"},{"doi-asserted-by":"publisher","key":"e_1_3_2_47_2","DOI":"10.1038\/jcbfm.1990.15"},{"doi-asserted-by":"publisher","key":"e_1_3_2_48_2","DOI":"10.1007\/BF00515596"},{"doi-asserted-by":"publisher","key":"e_1_3_2_49_2","DOI":"10.1038\/jcbfm.1989.41"},{"doi-asserted-by":"publisher","key":"e_1_3_2_50_2","DOI":"10.1016\/0306-4522(93)90508-D"},{"doi-asserted-by":"publisher","key":"e_1_3_2_51_2","DOI":"10.1016\/0304-3940(95)12057-B"},{"doi-asserted-by":"publisher","key":"e_1_3_2_52_2","DOI":"10.1016\/0006-8993(91)90873-T"},{"doi-asserted-by":"publisher","key":"e_1_3_2_53_2","DOI":"10.1016\/S0006-8993(97)00577-5"}],"container-title":["Stroke"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.STR.30.8.1695","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T08:26:14Z","timestamp":1741249574000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.STR.30.8.1695"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,8]]},"references-count":53,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1999,8]]}},"alternative-id":["10.1161\/01.STR.30.8.1695"],"URL":"https:\/\/doi.org\/10.1161\/01.str.30.8.1695","relation":{},"ISSN":["0039-2499","1524-4628"],"issn-type":[{"type":"print","value":"0039-2499"},{"type":"electronic","value":"1524-4628"}],"subject":[],"published":{"date-parts":[[1999,8]]},"assertion":[{"value":"1999-03-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-05-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}