{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T22:23:55Z","timestamp":1759616635785,"version":"3.38.0"},"reference-count":48,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2000,10]]},"abstract":"<jats:p><jats:italic>Background and Purpose<\/jats:italic>\u2014We postulated that some abnormalities in cerebrovascular function after subarachnoid hemorrhage (SAH) may involve underlying alterations in K<jats:sup>+<\/jats:sup>channel function. Thus, using pharmacological inhibitors, we assessed the influence of SAH on function of 2 types of K<jats:sup>+<\/jats:sup>channel in regulation of basilar artery diameter in vivo and membrane potential (E<jats:sub>m<\/jats:sub>) in vitro.<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>\u2014Rats were injected with saline (control) or autologous blood (SAH) into the cisterna magna. Two days later, effects of vasoactive drugs on the basilar artery were examined with a cranial window preparation. Vascular responses to 4-aminopyridine (4-AP), 3-aminopyridine (3-AP), tetraethylammonium (TEA), serotonin, acetylcholine, and adenosine were compared in control and SAH rats. Additional studies using intracellular microelectrodes evaluated the effects of 4-AP and serotonin on E<jats:sub>m<\/jats:sub>of basilar arteries isolated from control and SAH rats.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>\u2014Baseline artery diameter was 236\u00b15 \u03bcm in control rats and 220\u00b17 \u03bcm in SAH rats (<jats:italic>P<\/jats:italic>&lt;0.05). 4-AP, but not 3-AP, constricted the basilar artery in control rats, and responses to 4-AP were reduced in SAH rats. Constrictor responses to TEA or serotonin were unaffected by SAH. Vasodilator responses to acetylcholine were impaired in SAH rats, whereas responses to adenosine were not different. Resting E<jats:sub>m<\/jats:sub>was \u221281\u00b13 mV in control arteries and \u221279\u00b13 mV in SAH arteries. Both 4-AP and serotonin depolarized the basilar artery, but only 4-AP\u2013induced depolarization was impaired in SAH arteries.<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>\u2014These data suggest that 4-AP induces cerebral vasoconstriction in vivo through smooth muscle depolarization due to inhibition of voltage-dependent K<jats:sup>+<\/jats:sup>channels. Furthermore, function of these K<jats:sup>+<\/jats:sup>channels may be selectively reduced in the basilar artery after SAH and thus could contribute to cerebral vascular dysfunction.<\/jats:p>","DOI":"10.1161\/01.str.31.10.2460","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T20:08:03Z","timestamp":1308341283000},"page":"2460-2465","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":27,"title":["Selective Effects of Subarachnoid Hemorrhage on Cerebral Vascular Responses to 4-Aminopyridine in Rats"],"prefix":"10.1161","volume":"31","author":[{"given":"Lilly","family":"Quan","sequence":"first","affiliation":[{"name":"From the Department of Pharmacology, University of Melbourne, Parkville, Victoria, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher G.","family":"Sobey","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, University of Melbourne, Parkville, Victoria, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.268.4.C799"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199810000-00001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702439"},{"key":"e_1_3_2_4_2","first-page":"H1287","volume":"272","year":"1997","unstructured":"Paterno R, Heistad DD, Faraci FM. Functional activity of Ca2+-dependent K+ channels is increased in basilar artery during chronic hypertension. Am J Physiol.. 1997;272:H1287\u2013H1291.","journal-title":"Am J Physiol."},{"key":"e_1_3_2_5_2","first-page":"H256","volume":"272","year":"1997","unstructured":"Sobey CG, Faraci FM. Effect of nitric oxide and potassium channel agonists and inhibitors on basilar artery diameter. Am J Physiol.. 1997;272:H256\u2013H262.","journal-title":"Am J Physiol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1998.78.1.53"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.22.5.677"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(94)90172-4"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.81.6.996"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.25.1.8266368"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.27.10.1874"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.28.11.2273"},{"key":"e_1_3_2_13_2","first-page":"H126","volume":"271","year":"1996","unstructured":"Sobey CG, Heistad DD, Faraci FM. Effect of subarachnoid hemorrhage on dilatation of basilar artery in vivo. Am J Physiol.. 1996;271:H126\u2013H132.","journal-title":"Am J Physiol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.27.2.311"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.28.2.392"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.29.7.1469"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.82.6.729"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.22.8.1866764"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(94)00080-M"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1080\/02688699550041386"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.1998.tb02337.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113146"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1373909"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1995.269.1.H348"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1987.sp016566"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.267.6.C1589"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00370529"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00374731"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.76.6.1057"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.4.C1262"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)01284-X"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1995.83.3.0510"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1988.69.2.0239"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)01433-3"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.265.1.C299"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.27.9.1603"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0700880"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI5357"},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1016\/S0022-3565(24)37326-4","volume":"284","year":"1998","unstructured":"Salter KJ, Kozlowski RZ. Differential electrophysiological actions of endothelin-1 on Cl\u2212 and K+ currents in myocytes isolated from aorta, basilar and pulmonary artery. J Pharmacol Exp Ther.. 1998;284:1122\u20131131.","journal-title":"J Pharmacol Exp Ther."},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1998.274.5.L842"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.73.1.7685253"},{"key":"e_1_3_2_42_2","first-page":"H109","volume":"271","year":"1996","unstructured":"Aiello EA, Clement-Chomienne O, Sontag DP, Walsh MP, Cole WC. Protein kinase C inhibits delayed rectifier K+ current in rabbit vascular smooth muscle cells. Am J Physiol.. 1996;271:H109\u2013H119.","journal-title":"Am J Physiol."},{"key":"e_1_3_2_43_2","first-page":"697","volume":"31","year":"1992","unstructured":"Pasqualin A, Tsukahara T, Kassell NF, Torner JC. Effect of nicardipine on basilar artery vasoactive responses after subarachnoid hemorrhage. Neurosurgery.. 1992;31:697\u2013704.","journal-title":"Neurosurgery."},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1227\/00006123-198911000-00010"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.23.8.1636187"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1993.264.1.H178"},{"key":"e_1_4_3_1_1_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.268.4.C799"},{"key":"e_1_4_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199810000-00001"}],"container-title":["Stroke"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.STR.31.10.2460","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T08:06:26Z","timestamp":1741248386000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.STR.31.10.2460"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,10]]},"references-count":48,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2000,10]]}},"alternative-id":["10.1161\/01.STR.31.10.2460"],"URL":"https:\/\/doi.org\/10.1161\/01.str.31.10.2460","relation":{},"ISSN":["0039-2499","1524-4628"],"issn-type":[{"type":"print","value":"0039-2499"},{"type":"electronic","value":"1524-4628"}],"subject":[],"published":{"date-parts":[[2000,10]]},"assertion":[{"value":"2000-04-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-07-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}