{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T11:39:17Z","timestamp":1771069157976,"version":"3.50.1"},"reference-count":44,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2001,7]]},"abstract":"<jats:p>\n            <jats:italic>Background and Purpose<\/jats:italic>\n            \u2014The expression of inducible NO synthase (iNOS) after experimental subarachnoid hemorrhage (SAH) has been postulated to play a critical role in the pathogenesis of SAH and subsequent cerebral vasospasm. The inhibitory effect of CuZn-superoxide dismutase (CuZn-SOD) on the induction of iNOS after SAH was examined by using transgenic mice overexpressing CuZn-SOD.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Methods<\/jats:italic>\n            \u2014SOD-transgenic mice and nontransgenic littermates were subjected to SAH by endovascular perforation of the left anterior cerebral artery. The iNOS mRNA expression after SAH was determined by reverse transcription\u2013polymerase chain reaction, and the distribution of iNOS-positive cells was immunohistochemically examined. The nuclear expression of activated nuclear factor-\u03baB, a major transcription factor of iNOS gene, was also immunohistochemically examined.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Results<\/jats:italic>\n            \u2014In nontransgenic mice, SAH-induced iNOS protein and mRNA expressions in the arteries of basal cistern as well as in the cerebral cortex were demonstrated by immunohistochemistry and reverse transcription\u2013polymerase chain reaction. SAH-induced iNOS protein and mRNA expressions in those tissues were much reduced in SOD-transgenic mice compared with nontransgenic mice. Moreover, the nuclear expression of the activated form of nuclear factor-\u03baB was immunohistochemically detected in the cerebral cortices of nontransgenic mice but not in those of SOD-transgenic mice.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Conclusions<\/jats:italic>\n            \u2014These results indicate that oxygen-derived free radicals, particularly superoxide, play an important role in the iNOS gene expression after SAH and provide a molecular basis for the protective role of SOD against vasospasm after SAH.\n          <\/jats:p>","DOI":"10.1161\/01.str.32.7.1652","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T20:08:03Z","timestamp":1308341283000},"page":"1652-1657","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":39,"title":["Transgenic CuZn-Superoxide Dismutase Inhibits NO Synthase Induction in Experimental Subarachnoid Hemorrhage"],"prefix":"10.1161","volume":"32","author":[{"given":"Atsushi","family":"Saito","sequence":"first","affiliation":[{"name":"From the Departments of Neurosurgery (A.S., T.K., T.Y.) and Biochemistry (I.K., S.T., H.O.), Tohoku University Graduate School of Medicine, Sendai, Japan; the Department of Neurosurgery (H.K.), Yamagata University School of Medicine, Yamagata, Japan; and the Department of Neurosurgery and Neurology (P.H.C.), Stanford University, Palo Alto, Calif."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideyuki","family":"Kamii","sequence":"additional","affiliation":[{"name":"From the Departments of Neurosurgery (A.S., T.K., T.Y.) and Biochemistry (I.K., S.T., H.O.), Tohoku University Graduate School of Medicine, Sendai, Japan; the Department of Neurosurgery (H.K.), Yamagata University School of Medicine, Yamagata, Japan; and the Department of Neurosurgery and Neurology (P.H.C.), Stanford University, Palo Alto, Calif."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ichiro","family":"Kato","sequence":"additional","affiliation":[{"name":"From the Departments of Neurosurgery (A.S., T.K., T.Y.) and Biochemistry (I.K., S.T., H.O.), Tohoku University Graduate School of Medicine, Sendai, Japan; the Department of Neurosurgery (H.K.), Yamagata University School of Medicine, Yamagata, Japan; and the Department of Neurosurgery and Neurology (P.H.C.), Stanford University, Palo Alto, Calif."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin","family":"Takasawa","sequence":"additional","affiliation":[{"name":"From the Departments of Neurosurgery (A.S., T.K., T.Y.) and Biochemistry (I.K., S.T., H.O.), Tohoku University Graduate School of Medicine, Sendai, Japan; the Department of Neurosurgery (H.K.), Yamagata University School of Medicine, Yamagata, Japan; and the Department of Neurosurgery and Neurology (P.H.C.), Stanford University, Palo Alto, Calif."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeo","family":"Kondo","sequence":"additional","affiliation":[{"name":"From the Departments of Neurosurgery (A.S., T.K., T.Y.) and Biochemistry (I.K., S.T., H.O.), Tohoku University Graduate School of Medicine, Sendai, Japan; the Department of Neurosurgery (H.K.), Yamagata University School of Medicine, Yamagata, Japan; and the Department of Neurosurgery and Neurology (P.H.C.), Stanford University, Palo Alto, Calif."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pak H.","family":"Chan","sequence":"additional","affiliation":[{"name":"From the Departments of Neurosurgery (A.S., T.K., T.Y.) and Biochemistry (I.K., S.T., H.O.), Tohoku University Graduate School of Medicine, Sendai, Japan; the Department of Neurosurgery (H.K.), Yamagata University School of Medicine, Yamagata, Japan; and the Department of Neurosurgery and Neurology (P.H.C.), Stanford University, Palo Alto, Calif."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Okamoto","sequence":"additional","affiliation":[{"name":"From the Departments of Neurosurgery (A.S., T.K., T.Y.) and Biochemistry (I.K., S.T., H.O.), Tohoku University Graduate School of Medicine, Sendai, Japan; the Department of Neurosurgery (H.K.), Yamagata University School of Medicine, Yamagata, Japan; and the Department of Neurosurgery and Neurology (P.H.C.), Stanford University, Palo Alto, Calif."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Yoshimoto","sequence":"additional","affiliation":[{"name":"From the Departments of Neurosurgery (A.S., T.K., T.Y.) and Biochemistry (I.K., S.T., H.O.), Tohoku University Graduate School of Medicine, Sendai, Japan; the Department of Neurosurgery (H.K.), Yamagata University School of Medicine, Yamagata, Japan; and the Department of Neurosurgery and Neurology (P.H.C.), Stanford University, Palo Alto, Calif."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_1_1_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1996.84.5.0792"},{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(94)90064-7"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.25.4.8160234"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.30.4.867"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/01616412.1998.11740488"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/01616412.1999.11740934"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1999.90.6.1098"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2496"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(92)91004-X"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.149.8.2736"},{"key":"e_1_3_1_11_2","first-page":"2829","volume":"140","year":"1998","unstructured":"Drapier JC, Hibbs JB Jr. Differentiation of murine macrophages to express nonspecific cytotoxicity for tumor cells results in L-arginine-dependent inhibition of mitochondrial iron-sulfur enzymes in the macrophage effector cells. J Immunol. 1998;140:2829\u20132838.","journal-title":"J Immunol"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.95.2.527"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90286-4"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(00)70268-X"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-200003000-00017"},{"key":"e_1_3_1_16_2","first-page":"8044","volume":"83","year":"1987","unstructured":"Epstein CJ, Avraham KB, Lovett M, Smith S, Elory-Stein O, Rotman G, Bry C, Groner Y. Transgenic mice with increased CuZn-superoxide dismutase activity: animal model of dosage effects in Down syndrome. Proc Natl Acad Sci U S A. 1987;83:8044\u20138048.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6421"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410290506"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199611000-00009"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(94)90818-4"},{"key":"e_1_3_1_21_2","unstructured":"Kamii H Kinouchi H Sharp FR Chan PH. A model of transient focal cerebral ischemia in the mouse. In: Ohnishi ST Ohnishi T eds. Central Nervous System Trauma: Research Techniques. Boca Raton Fla: CRC Press; 1995:139\u2013146."},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.25.1.8266365"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)00924-9"},{"key":"e_1_3_1_24_2","first-page":"81","volume":"191","year":"1993","unstructured":"Nunokawa Y, Ishida N, Tanaka S. Cloning of inducible nitric oxide synthase in rat vascular smooth muscle cells. Biochem Biophys Res Commun. 1993;191:81\u201394.","journal-title":"Biochem Biophys Res Commun"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/13.7.2485"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-23-09157.1997"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1995.47"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.26.12041"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(95)00578-1"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.4.1425"},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0999"},{"key":"e_1_3_1_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(95)00158-1"},{"key":"e_1_3_1_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/8432"},{"key":"e_1_3_1_34_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.4.719"},{"key":"e_1_3_1_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.21.9618"},{"key":"e_1_3_1_36_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.12.040194.001041"},{"key":"e_1_4_3_1_1_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.25.4.8160234"},{"key":"e_1_4_3_1_2_2","first-page":"529","volume":"30","year":"1992","unstructured":"Macdonald RL, Weir BK, Runzer TD, Grace M, G, Poznansky MJ. Effect of intrathecal superoxide dismutase and catalase on oxyhemoglobin-induced vasospasm in monkeys. Neurosurgery. 1992;30:529\u2013539.","journal-title":"Neurosurgery"},{"key":"e_1_4_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1227\/00006123-199101000-00005"},{"key":"e_1_4_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1998.88.2.0298"},{"key":"e_1_4_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1227\/00006123-198902000-00005"},{"key":"e_1_4_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1989.71.1.0098"},{"key":"e_1_4_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/01616412.1984.11739663"},{"key":"e_1_4_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.32.3.636"}],"container-title":["Stroke"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.STR.32.7.1652","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T22:28:30Z","timestamp":1715552910000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.STR.32.7.1652"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,7]]},"references-count":44,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2001,7]]}},"alternative-id":["10.1161\/01.STR.32.7.1652"],"URL":"https:\/\/doi.org\/10.1161\/01.str.32.7.1652","relation":{},"ISSN":["0039-2499","1524-4628"],"issn-type":[{"value":"0039-2499","type":"print"},{"value":"1524-4628","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,7]]},"assertion":[{"value":"2000-09-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-04-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}