{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:39:05Z","timestamp":1790861945566,"version":"4.1.0"},"reference-count":43,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:p>\n                    <jats:bold>\n                      <jats:italic>Objective\u2014<\/jats:italic>\n                    <\/jats:bold>\n                    An assay proposed to quantify endothelial progenitor cell (EPC) colonies in humans was investigated to determine the phenotype of recovered cells and their relevance to in vivo endothelial function.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>\n                      <jats:italic>Methods and Results\u2014<\/jats:italic>\n                    <\/jats:bold>\n                    Twelve sedentary subjects participating in a worksite wellness program underwent endothelial flow-mediated dilation (FMD) testing of the brachial artery and blood sampling for EPC colony assay. Microarray-based genotypic characterization of colonies showed surface markers consistent with T lymphocyte phenotype, but not with an EPC (CD34, CD133, VEGFR-2) or endothelial (CD146) phenotype. Gene expression patterns more closely matched T lymphocytes (\n                    <jats:italic>r<\/jats:italic>\n                    =0.87) than endothelial cells (\n                    <jats:italic>r<\/jats:italic>\n                    =0.66) in our microarray database. Flow cytometry of colonies confirmed large populations of CD3+CD45+ T cells (&gt;75%) and few CD146+CD45\u2212 endothelial cells (&lt;1%). Further, there was no correlation between colony number and the magnitude of FMD (\n                    <jats:italic>r<\/jats:italic>\n                    =\u22120.1512,\n                    <jats:italic>P<\/jats:italic>\n                    =0.6389). After exercise training, subjects improved FMD, from 6.7\u00b12.0 to 8.7\u00b11.9% (\n                    <jats:italic>P<\/jats:italic>\n                    =0.0043). Colonies also increased (\n                    <jats:italic>P<\/jats:italic>\n                    =0.0210), but without relation to FMD (\n                    <jats:italic>r<\/jats:italic>\n                    =0.1074,\n                    <jats:italic>P<\/jats:italic>\n                    =0.7396). T lymphocyte phenotype persisted after exercise (\n                    <jats:italic>r<\/jats:italic>\n                    =0.87).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>\n                      <jats:italic>Conclusions\u2014<\/jats:italic>\n                    <\/jats:bold>\n                    Cells in a commonly used EPC colony assay have a gene expression and cell surface marker profile consistent with a predominance of T lymphocytes and have an unclear relevance to endothelial function, either before or after exercise training.\n                  <\/jats:p>","DOI":"10.1161\/atvbaha.108.174573","type":"journal-article","created":{"date-parts":[[2008,12,17]],"date-time":"2008-12-17T17:55:34Z","timestamp":1229536534000},"page":"121-127","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":31,"title":["Microarray-Based Characterization of a Colony Assay Used to Investigate Endothelial Progenitor Cells and Relevance to Endothelial Function in Humans"],"prefix":"10.1161","volume":"29","author":[{"given":"Aditi","family":"Desai","sequence":"first","affiliation":[{"name":"From the Translational Medicine Branch (A.D., A.G., A.B., G.Z., M.L., M.A.S., R.O.C.), Genomics Core (D.L., N.R., P.J.M.), and Flow Cytometry Core (J.P.M.), National Heart, Lung, and Blood Institute; and the Functional Genomics and Proteomic Facility (N.R., M.A.S., R.L.D.), Critical Care Medicine Department, NIH Clinical Center; National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Glaser","sequence":"additional","affiliation":[{"name":"From the Translational Medicine Branch (A.D., A.G., A.B., G.Z., M.L., M.A.S., R.O.C.), Genomics Core (D.L., N.R., P.J.M.), and Flow Cytometry Core (J.P.M.), National Heart, Lung, and Blood Institute; and the Functional Genomics and Proteomic Facility (N.R., M.A.S., R.L.D.), Critical Care Medicine Department, NIH Clinical Center; National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delong","family":"Liu","sequence":"additional","affiliation":[{"name":"From the Translational Medicine Branch (A.D., A.G., A.B., G.Z., M.L., M.A.S., R.O.C.), Genomics Core (D.L., N.R., P.J.M.), and Flow Cytometry Core (J.P.M.), National Heart, Lung, and Blood Institute; and the Functional Genomics and Proteomic Facility (N.R., M.A.S., R.L.D.), Critical Care Medicine Department, NIH Clinical Center; National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nalini","family":"Raghavachari","sequence":"additional","affiliation":[{"name":"From the Translational Medicine Branch (A.D., A.G., A.B., G.Z., M.L., M.A.S., R.O.C.), Genomics Core (D.L., N.R., P.J.M.), and Flow Cytometry Core (J.P.M.), National Heart, Lung, and Blood Institute; and the Functional Genomics and Proteomic Facility (N.R., M.A.S., R.L.D.), Critical Care Medicine Department, NIH Clinical Center; National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnon","family":"Blum","sequence":"additional","affiliation":[{"name":"From the Translational Medicine Branch (A.D., A.G., A.B., G.Z., M.L., M.A.S., R.O.C.), Genomics Core (D.L., N.R., P.J.M.), and Flow Cytometry Core (J.P.M.), National Heart, Lung, and Blood Institute; and the Functional Genomics and Proteomic Facility (N.R., M.A.S., R.L.D.), Critical Care Medicine Department, NIH Clinical Center; National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria","family":"Zalos","sequence":"additional","affiliation":[{"name":"From the Translational Medicine Branch (A.D., A.G., A.B., G.Z., M.L., M.A.S., R.O.C.), Genomics Core (D.L., N.R., P.J.M.), and Flow Cytometry Core (J.P.M.), National Heart, Lung, and Blood Institute; and the Functional Genomics and Proteomic Facility (N.R., M.A.S., R.L.D.), Critical Care Medicine Department, NIH Clinical Center; National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret","family":"Lippincott","sequence":"additional","affiliation":[{"name":"From the Translational Medicine Branch (A.D., A.G., A.B., G.Z., M.L., M.A.S., R.O.C.), Genomics Core (D.L., N.R., P.J.M.), and Flow Cytometry Core (J.P.M.), National Heart, Lung, and Blood Institute; and the Functional Genomics and Proteomic Facility (N.R., M.A.S., R.L.D.), Critical Care Medicine Department, NIH Clinical Center; National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Philip","family":"McCoy","sequence":"additional","affiliation":[{"name":"From the Translational Medicine Branch (A.D., A.G., A.B., G.Z., M.L., M.A.S., R.O.C.), Genomics Core (D.L., N.R., P.J.M.), and Flow Cytometry Core (J.P.M.), National Heart, Lung, and Blood Institute; and the Functional Genomics and Proteomic Facility (N.R., M.A.S., R.L.D.), Critical Care Medicine Department, NIH Clinical Center; National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Munson","sequence":"additional","affiliation":[{"name":"From the Translational Medicine Branch (A.D., A.G., A.B., G.Z., M.L., M.A.S., R.O.C.), Genomics Core (D.L., N.R., P.J.M.), and Flow Cytometry Core (J.P.M.), National Heart, Lung, and Blood Institute; and the Functional Genomics and Proteomic Facility (N.R., M.A.S., R.L.D.), Critical Care Medicine Department, NIH Clinical Center; National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Solomon","sequence":"additional","affiliation":[{"name":"From the Translational Medicine Branch (A.D., A.G., A.B., G.Z., M.L., M.A.S., R.O.C.), Genomics Core (D.L., N.R., P.J.M.), and Flow Cytometry Core (J.P.M.), National Heart, Lung, and Blood Institute; and the Functional Genomics and Proteomic Facility (N.R., M.A.S., R.L.D.), Critical Care Medicine Department, NIH Clinical Center; National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L.","family":"Danner","sequence":"additional","affiliation":[{"name":"From the Translational Medicine Branch (A.D., A.G., A.B., G.Z., M.L., M.A.S., R.O.C.), Genomics Core (D.L., N.R., P.J.M.), and Flow Cytometry Core (J.P.M.), National Heart, Lung, and Blood Institute; and the Functional Genomics and Proteomic Facility (N.R., M.A.S., R.L.D.), Critical Care Medicine Department, NIH Clinical Center; National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"Richard O.","family":"Cannon","sequence":"additional","affiliation":[{"name":"From the Translational Medicine Branch (A.D., A.G., A.B., G.Z., M.L., M.A.S., R.O.C.), Genomics Core (D.L., N.R., P.J.M.), and Flow Cytometry Core (J.P.M.), National Heart, Lung, and Blood Institute; and the Functional Genomics and Proteomic Facility (N.R., M.A.S., R.L.D.), Critical Care Medicine Department, NIH Clinical Center; National Institutes of Health, Bethesda, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5302.964"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.85.3.221"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V92.2.362"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.3.952.003k27_952_958"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/7434"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.88.2.167"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.10.3106"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm948"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa022287"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000058702.69484.a0"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000096483.29777.50"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-08-043471"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.107.694778"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.7.5141"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0833"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404676"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.57.4.523"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2004.02.049"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000109141.48980.37"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2005.01.006"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.HCR.0000265031.10145.50"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2008.05.020"},{"key":"e_1_3_3_23_2","unstructured":"Munson PJ. 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