{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:59:02Z","timestamp":1791219542404,"version":"4.1.0"},"reference-count":41,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Objective\u2014<\/jats:italic>\n            <\/jats:bold>\n            Relationships between intracellular Ca\n            <jats:sup>2+<\/jats:sup>\n            concentration ([Ca\n            <jats:sup>2+<\/jats:sup>\n            ]\n            <jats:sub>cyt<\/jats:sub>\n            ) and apoptotic events, such as mitochondrial depolarization (\u0394\u03a8m loss) and Bcl-2 and Bad phosphorylation, were analyzed in platelets and Jurkat cells in relation to rapid procoagulant phosphatidylserine (PS) exposure.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods and Results\u2014<\/jats:italic>\n            <\/jats:bold>\n            Platelets were stimulated with A23187, thapsigargin (TG) and thrombin plus convulxin (Thr\/Cvx), and Jurkat cells with ionomycin, in the presence or absence of cyclosporin A (CsA), a mitochondrial permeability transition pore inhibitor. \u0394\u03a8m loss occurred when platelets were stimulated in Ca\n            <jats:sup>2+<\/jats:sup>\n            medium in conditions exposing PS, but also in EGTA medium. CsA inhibited PS exposure, [Ca\n            <jats:sup>2+<\/jats:sup>\n            ]\n            <jats:sub>cyt<\/jats:sub>\n            increase, and \u0394\u03a8m loss in platelets stimulated with TG and Thr\/Cvx, but had no inhibitory effect on A23187 stimulation. CsA reduced TG-induced Ca\n            <jats:sup>2+<\/jats:sup>\n            release from the endoplasmic reticulum and, consequently, external Ca\n            <jats:sup>2+<\/jats:sup>\n            influx. In ionomycin-stimulated Jurkat cells, rapid PS exposure was evidenced but not \u0394\u03a8m loss, and CsA did not inhibit the process. The status of phosphorylated Bad and Bcl-2 in both cell types remained unchanged on stimulation.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusions\u2014<\/jats:italic>\n            <\/jats:bold>\n            Whether \u0394\u03a8m loss occurs or not, PS exposure is triggered by a high [Ca\n            <jats:sup>2+<\/jats:sup>\n            ]\n            <jats:sub>cyt<\/jats:sub>\n            increase. Data further demonstrate that CsA prevents membrane scrambling by inhibiting the high [Ca\n            <jats:sup>2+<\/jats:sup>\n            ]\n            <jats:sub>cyt<\/jats:sub>\n            increase, independently of its effect on mitochondrial permeability transition pore.\n          <\/jats:p>","DOI":"10.1161\/atvbaha.109.190926","type":"journal-article","created":{"date-parts":[[2009,8,21]],"date-time":"2009-08-21T02:18:49Z","timestamp":1250821129000},"page":"1883-1889","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":43,"title":["Rapid Procoagulant Phosphatidylserine Exposure Relies on High Cytosolic Calcium Rather Than on Mitochondrial Depolarization"],"prefix":"10.1161","volume":"29","author":[{"given":"Amal","family":"Arachiche","sequence":"first","affiliation":[{"name":"From INSERM U688 and Universite\u0301 Victor Segalen (A.A., T.L., J.D.-P.), Bordeaux, INSERM U770 and Universite\u0301 Paris-Sud (A.A., D.K.-N.), Le Kremlin-Bice\u0302tre, INSERM UMR-S757 and Universite\u0301 Paris-Sud (I.G.), Orsay, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danie\u0300le","family":"Kerbiriou-Nabias","sequence":"additional","affiliation":[{"name":"From INSERM U688 and Universite\u0301 Victor Segalen (A.A., T.L., J.D.-P.), Bordeaux, INSERM U770 and Universite\u0301 Paris-Sud (A.A., D.K.-N.), Le Kremlin-Bice\u0302tre, INSERM UMR-S757 and Universite\u0301 Paris-Sud (I.G.), Orsay, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Garcin","sequence":"additional","affiliation":[{"name":"From INSERM U688 and Universite\u0301 Victor Segalen (A.A., T.L., J.D.-P.), Bordeaux, INSERM U770 and Universite\u0301 Paris-Sud (A.A., D.K.-N.), Le Kremlin-Bice\u0302tre, INSERM UMR-S757 and Universite\u0301 Paris-Sud (I.G.), Orsay, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Letellier","sequence":"additional","affiliation":[{"name":"From INSERM U688 and Universite\u0301 Victor Segalen (A.A., T.L., J.D.-P.), Bordeaux, INSERM U770 and Universite\u0301 Paris-Sud (A.A., D.K.-N.), Le Kremlin-Bice\u0302tre, INSERM UMR-S757 and Universite\u0301 Paris-Sud (I.G.), Orsay, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanne","family":"Dachary-Prigent","sequence":"additional","affiliation":[{"name":"From INSERM U688 and Universite\u0301 Victor Segalen (A.A., T.L., J.D.-P.), Bordeaux, INSERM U770 and Universite\u0301 Paris-Sud (A.A., D.K.-N.), Le Kremlin-Bice\u0302tre, INSERM UMR-S757 and Universite\u0301 Paris-Sud (I.G.), Orsay, 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