{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T23:01:18Z","timestamp":1773270078298,"version":"3.50.1"},"reference-count":22,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Objective\u2014<\/jats:italic>\n            <\/jats:bold>\n            Phospholipase C-\u03b41 activity is enhanced in patients with coronary artery spasm, and a p122 protein was recently cloned to potentiate phospholipase C-\u03b41 activity. To investigate the role of p122 in enhanced vasomotility, we examined p122 expression in the cultured skin fibroblasts obtained from patients with and without coronary spasm, intracellular Ca\n            <jats:sup>2+<\/jats:sup>\n            concentration ([Ca\n            <jats:sup>2+<\/jats:sup>\n            ]\n            <jats:sub>i<\/jats:sub>\n            ) at baseline and after stimulation with acetylcholine in the cells transfected with p122, and promoter in genomic DNA.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods and Results\u2014<\/jats:italic>\n            <\/jats:bold>\n            p122 protein and gene expression levels in patients with coronary spasm (n=11) were enhanced compared with levels in control subjects (n=9) (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.01 for both). [Ca\n            <jats:sup>2+<\/jats:sup>\n            ]\n            <jats:sub>i<\/jats:sub>\n            at baseline and the peak increase in [Ca\n            <jats:sup>2+<\/jats:sup>\n            ]\n            <jats:sub>i<\/jats:sub>\n            in response to acetylcholine were both 2 times higher in cells transfected with p122 than in those without p122. Conversely, knockdown of p122 resulted in diminished [Ca\n            <jats:sup>2+<\/jats:sup>\n            ]\n            <jats:sub>i<\/jats:sub>\n            response. In the p122 promoter analysis, the \u2212228G\/A and \u22121466C\/T variants revealed the increase in luciferase activity. Although the \u22121466C\/T variant was similar between 144 patients with coronary spasm and 148 controls, the \u2212228G\/A variant was more frequent in male patients than in male controls (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.05).\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusion\u2014<\/jats:italic>\n            <\/jats:bold>\n            The p122 protein is upregulated in patients with coronary spasm, causing increased [Ca\n            <jats:sup>2+<\/jats:sup>\n            ]\n            <jats:sub>i<\/jats:sub>\n            to acetylcholine, and thereby seems to be related to enhanced coronary vasomotility.\n          <\/jats:p>","DOI":"10.1161\/atvbaha.110.203083","type":"journal-article","created":{"date-parts":[[2010,7,16]],"date-time":"2010-07-16T02:55:55Z","timestamp":1279248955000},"page":"1968-1975","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":10,"title":["p122 Protein Enhances Intracellular Calcium Increase to Acetylcholine"],"prefix":"10.1161","volume":"30","author":[{"given":"Reiichi","family":"Murakami","sequence":"first","affiliation":[{"name":"From the Department of Cardiology (R.M., T.O., H.T., S.S., and K.O.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; The Center for Advanced Medical Research (A.M. and K.I.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; and the Department of Biomolecular Science (Y.H.), Fukushima Medical University School of Medicine, Fukushima, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiro","family":"Osanai","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology (R.M., T.O., H.T., S.S., and K.O.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; The Center for Advanced Medical Research (A.M. and K.I.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; and the Department of Biomolecular Science (Y.H.), Fukushima Medical University School of Medicine, Fukushima, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirofumi","family":"Tomita","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology (R.M., T.O., H.T., S.S., and K.O.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; The Center for Advanced Medical Research (A.M. and K.I.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; and the Department of Biomolecular Science (Y.H.), Fukushima Medical University School of Medicine, Fukushima, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoko","family":"Sasaki","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology (R.M., T.O., H.T., S.S., and K.O.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; The Center for Advanced Medical Research (A.M. and K.I.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; and the Department of Biomolecular Science (Y.H.), Fukushima Medical University School of Medicine, Fukushima, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Maruyama","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology (R.M., T.O., H.T., S.S., and K.O.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; The Center for Advanced Medical Research (A.M. and K.I.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; and the Department of Biomolecular Science (Y.H.), Fukushima Medical University School of Medicine, Fukushima, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"Itoh","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology (R.M., T.O., H.T., S.S., and K.O.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; The Center for Advanced Medical Research (A.M. and K.I.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; and the Department of Biomolecular Science (Y.H.), Fukushima Medical University School of Medicine, Fukushima, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimi","family":"Homma","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology (R.M., T.O., H.T., S.S., and K.O.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; The Center for Advanced Medical Research (A.M. and K.I.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; and the Department of Biomolecular Science (Y.H.), Fukushima Medical University School of Medicine, Fukushima, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"Okumura","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology (R.M., T.O., H.T., S.S., and K.O.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; The Center for Advanced Medical Research (A.M. and K.I.), Hirosaki University Graduate School of Medicine, Hirosaki, Japan; and the Department of Biomolecular Science (Y.H.), Fukushima Medical University School of Medicine, Fukushima, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197304122881501"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.50.3.534"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197812072992303"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)90651-3"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bb.17.060188.000323"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00786.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.37.26127"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01396.2007"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2008.03.007"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(00)00966-9"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000014613.36469.3f"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.25.17757"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1356-9597.2004.00698.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb07002.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06387.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.19.5.446"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(95)00432-7"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(88)90449-4"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(89)90100-9"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.74.6.3779913"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.105.13.1545"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000090998.08629.60"}],"updated-by":[{"DOI":"10.1161\/atv.0b013e3181fdc67e","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000}}],"container-title":["Arteriosclerosis, Thrombosis, and Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/ATVBAHA.110.203083","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T22:53:01Z","timestamp":1715554381000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/ATVBAHA.110.203083"}},"subtitle":["Its Possible Role in the Pathogenesis of Coronary Spastic Angina"],"short-title":[],"issued":{"date-parts":[[2010,10]]},"references-count":22,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2010,10]]}},"alternative-id":["10.1161\/ATVBAHA.110.203083"],"URL":"https:\/\/doi.org\/10.1161\/atvbaha.110.203083","relation":{"correction":[{"id-type":"doi","id":"10.1161\/ATV.0b013e3181fdc67e","asserted-by":"object"}]},"ISSN":["1079-5642","1524-4636"],"issn-type":[{"value":"1079-5642","type":"print"},{"value":"1524-4636","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10]]},"assertion":[{"value":"2010-07-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}