{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:25:30Z","timestamp":1791321930447,"version":"4.3.1"},"reference-count":34,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"12","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Objective\u2014<\/jats:italic>\n            <\/jats:bold>\n            To assess the coronary endothelial protective effects of 17\u03b2-estradiol (E2) and the role of estrogen receptor (ER) \u03b1 in ischemia\/reperfusion (I\/R).\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods and Results\u2014<\/jats:italic>\n            <\/jats:bold>\n            E2 exerts protective effects in cardiac I\/R. However, the implication in vivo of the endothelium and the cellular targets of the anti-ischemic effects of E2 are unknown. Mice were subjected to I\/R (30 minutes of I and 1 hour of R) in vivo, after which acetylcholine-induced relaxation of isolated coronary segments was assessed ex vivo. I\/R induced a coronary endothelial dysfunction in untreated ovariectomized mice that was prevented by long-term treatment with E2 in wild-type, but not in ER\u03b1\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            , mice. Chimeric mice inactivated for ER\u03b1 in the hematopoietic compartment remained protected by E2. Further inactivation of endothelial ER\u03b1 abolished the protective action of E2 on coronary endothelial function in Tie2-Cre(+) ER\u03b1\n            <jats:sup>f\/f<\/jats:sup>\n            mice. More importantly, E2 significantly limited infarct size in wild-type mice but not in mice deficient in endothelial ER\u03b1, even in the presence of hematopoietic ER\u03b1.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusion\u2014<\/jats:italic>\n            <\/jats:bold>\n            Endothelial ER\u03b1 plays a crucial role in the E2-induced prevention of endothelial dysfunction after I\/R. To our knowledge, we demonstrate for the first time, by using unique genetically modified mice, that targeting endothelial protection per se can confer cardiomyocyte protection in I\/R.\n          <\/jats:p>","DOI":"10.1161\/atvbaha.110.213637","type":"journal-article","created":{"date-parts":[[2010,9,17]],"date-time":"2010-09-17T04:31:47Z","timestamp":1284697907000},"page":"2562-2567","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":62,"title":["Endothelial Estrogen Receptor \u03b1 Plays an Essential Role in the Coronary and Myocardial Protective Effects of Estradiol in Ischemia\/Reperfusion"],"prefix":"10.1161","volume":"30","author":[{"given":"Julie","family":"Favre","sequence":"first","affiliation":[{"name":"From the Department of Pharmacology, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U644 and Rouen University Hospital (J.F., J.G., J.-P.H., I.R.-J., C.T., and V.R.), Institute for Biomedical Research Institut Fe\u0301de\u0301ratif de Recherches, University of Rouen, France; INSERM U858 (J.F., A.B.-G., J.-F.A., and F.L.), Institut de Me\u0301decine Mole\u0301culaire de Rangueil, University of Toulouse III, Toulouse, France; Centre de Microscopie Electronique Applique\u0301e a\u0300 la Biologie (I.F.), Faculty of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Gao","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U644 and Rouen University Hospital (J.F., J.G., J.-P.H., I.R.-J., C.T., and V.R.), Institute for Biomedical Research Institut Fe\u0301de\u0301ratif de Recherches, University of Rouen, France; INSERM U858 (J.F., A.B.-G., J.-F.A., and F.L.), Institut de Me\u0301decine Mole\u0301culaire de Rangueil, University of Toulouse III, Toulouse, France; Centre de Microscopie Electronique Applique\u0301e a\u0300 la Biologie (I.F.), Faculty of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Paul","family":"Henry","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U644 and Rouen University Hospital (J.F., J.G., J.-P.H., I.R.-J., C.T., and V.R.), Institute for Biomedical Research Institut Fe\u0301de\u0301ratif de Recherches, University of Rouen, France; INSERM U858 (J.F., A.B.-G., J.-F.A., and F.L.), Institut de Me\u0301decine Mole\u0301culaire de Rangueil, University of Toulouse III, Toulouse, France; Centre de Microscopie Electronique Applique\u0301e a\u0300 la Biologie (I.F.), Faculty of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Remy-Jouet","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U644 and Rouen University Hospital (J.F., J.G., J.-P.H., I.R.-J., C.T., and V.R.), Institute for Biomedical Research Institut Fe\u0301de\u0301ratif de Recherches, University of Rouen, France; INSERM U858 (J.F., A.B.-G., J.-F.A., and F.L.), Institut de Me\u0301decine Mole\u0301culaire de Rangueil, University of Toulouse III, Toulouse, France; Centre de Microscopie Electronique Applique\u0301e a\u0300 la Biologie (I.F.), Faculty of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Fourquaux","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U644 and Rouen University Hospital (J.F., J.G., J.-P.H., I.R.-J., C.T., and V.R.), Institute for Biomedical Research Institut Fe\u0301de\u0301ratif de Recherches, University of Rouen, France; INSERM U858 (J.F., A.B.-G., J.-F.A., and F.L.), Institut de Me\u0301decine Mole\u0301culaire de Rangueil, University of Toulouse III, Toulouse, France; Centre de Microscopie Electronique Applique\u0301e a\u0300 la Biologie (I.F.), Faculty of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Billon-Gales","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U644 and Rouen University Hospital (J.F., J.G., J.-P.H., I.R.-J., C.T., and V.R.), Institute for Biomedical Research Institut Fe\u0301de\u0301ratif de Recherches, University of Rouen, France; INSERM U858 (J.F., A.B.-G., J.-F.A., and F.L.), Institut de Me\u0301decine Mole\u0301culaire de Rangueil, University of Toulouse III, Toulouse, France; Centre de Microscopie Electronique Applique\u0301e a\u0300 la Biologie (I.F.), Faculty of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Thuillez","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U644 and Rouen University Hospital (J.F., J.G., J.-P.H., I.R.-J., C.T., and V.R.), Institute for Biomedical Research Institut Fe\u0301de\u0301ratif de Recherches, University of Rouen, France; INSERM U858 (J.F., A.B.-G., J.-F.A., and F.L.), Institut de Me\u0301decine Mole\u0301culaire de Rangueil, University of Toulouse III, Toulouse, France; Centre de Microscopie Electronique Applique\u0301e a\u0300 la Biologie (I.F.), Faculty of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Franc\u0327ois","family":"Arnal","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U644 and Rouen University Hospital (J.F., J.G., J.-P.H., I.R.-J., C.T., and V.R.), Institute for Biomedical Research Institut Fe\u0301de\u0301ratif de Recherches, University of Rouen, France; INSERM U858 (J.F., A.B.-G., J.-F.A., and F.L.), Institut de Me\u0301decine Mole\u0301culaire de Rangueil, University of Toulouse III, Toulouse, France; Centre de Microscopie Electronique Applique\u0301e a\u0300 la Biologie (I.F.), Faculty of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327oise","family":"Lenfant","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U644 and Rouen University Hospital (J.F., J.G., J.-P.H., I.R.-J., C.T., and V.R.), Institute for Biomedical Research Institut Fe\u0301de\u0301ratif de Recherches, University of Rouen, France; INSERM U858 (J.F., A.B.-G., J.-F.A., and F.L.), Institut de Me\u0301decine Mole\u0301culaire de Rangueil, University of Toulouse III, Toulouse, France; Centre de Microscopie Electronique Applique\u0301e a\u0300 la Biologie (I.F.), Faculty of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Richard","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U644 and Rouen University Hospital (J.F., J.G., J.-P.H., I.R.-J., C.T., and V.R.), Institute for Biomedical Research Institut Fe\u0301de\u0301ratif de Recherches, University of Rouen, France; INSERM U858 (J.F., A.B.-G., J.-F.A., and F.L.), Institut de Me\u0301decine Mole\u0301culaire de Rangueil, University of Toulouse III, Toulouse, France; Centre de Microscopie Electronique Applique\u0301e a\u0300 la Biologie (I.F.), Faculty of..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1997.0476"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00479.2005"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.109.868729"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/atvbaha.107.140723"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.90.4.413"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.103.3.423"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11320"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808742106"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.108.182410"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00045.2009"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.109.191221"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1621\/nrs.04013"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116501"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.19.4277"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1161\/atvbaha.109.192849"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.107.722009"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.109.898445"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.str.0000054051.88378.25"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1996.0093"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(97)01201-6"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.8.2957-2968.2005"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.12.7980"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1677\/JOE-08-0071"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.278.5.H1640"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01219.2005"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.105.553925"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.11.6503"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20643"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-201X.2003.01068.x"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.86.3.1355412"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.108.805242"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M511024200"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2009.04.026"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2007.01.014"}],"container-title":["Arteriosclerosis, Thrombosis, and Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/ATVBAHA.110.213637","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T23:02:37Z","timestamp":1715554957000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/ATVBAHA.110.213637"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12]]},"references-count":34,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["10.1161\/ATVBAHA.110.213637"],"URL":"https:\/\/doi.org\/10.1161\/atvbaha.110.213637","relation":{},"ISSN":["1079-5642","1524-4636"],"issn-type":[{"value":"1079-5642","type":"print"},{"value":"1524-4636","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12]]},"assertion":[{"value":"2010-09-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}