{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T15:19:37Z","timestamp":1791991177392,"version":"4.4.0"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:sec>\n            <jats:title>Objective\u2014<\/jats:title>\n            <jats:p>The proinflammatory cytokine S100A12 is associated with coronary atherosclerotic plaque rupture. We previously generated transgenic mice with vascular smooth muscle\u2013targeted expression of human S100A12 and found that these mice developed aortic aneurysmal dilation of the thoracic aorta. In the current study, we tested the hypothesis that S100A12 expressed in vascular smooth muscle in atherosclerosis-prone apolipoprotein E (ApoE)\u2013null mice would accelerate atherosclerosis.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods and Results\u2014<\/jats:title>\n            <jats:p>ApoE-null mice with or without the S100A12 transgene were analyzed. We found a 1.4-fold increase in atherosclerotic plaque size and more specifically a large increase in calcified plaque area (45% versus 7% of innominate artery plaques and 18% versus 10% of aortic root plaques) in S100A12\/ApoE-null mice compared with wild-type\/ApoE-null littermates. Expression of bone morphogenic protein and other osteoblastic genes was increased in aorta and cultured vascular smooth muscle, and importantly, these changes in gene expression preceded the development of vascular calcification in S100A12\/ApoE-null mice. Accelerated atherosclerosis and vascular calcification were mediated, at least in part, by oxidative stress because inhibition of NADPH oxidase attenuated S100A12-mediated osteogenesis in cultured vascular smooth muscle cells. S100A12 transgenic mice in the wild-type background (ApoE+\/+) showed minimal vascular calcification, suggesting that S100A12 requires a proinflammatory\/proatherosclerotic environment to induce osteoblastic differentiation and vascular calcification.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion\u2014<\/jats:title>\n            <jats:p>Vascular smooth muscle S100A12 accelerates atherosclerosis and augments atherosclerosis-triggered osteogenesis, reminiscent of features associated with plaque instability.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/atvbaha.110.217745","type":"journal-article","created":{"date-parts":[[2010,10,22]],"date-time":"2010-10-22T05:27:35Z","timestamp":1287725255000},"page":"337-344","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":102,"title":["S100A12 in Vascular Smooth Muscle Accelerates Vascular Calcification in Apolipoprotein E\u2013Null Mice by Activating an Osteogenic Gene Regulatory Program"],"prefix":"10.1161","volume":"31","author":[{"given":"Marion A.","family":"Hofmann Bowman","sequence":"first","affiliation":[{"name":"From the Department of Medicine, Section of Cardiology (M.A.H.B., J.G., U.B., P.T.T., G.K., J.E., E.M.M.) and Department of Pathology (A.N.H.), University of Chicago, Chicago, Ill."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Gawdzik","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Section of Cardiology (M.A.H.B., J.G., U.B., P.T.T., G.K., J.E., E.M.M.) and Department of Pathology (A.N.H.), University of Chicago, Chicago, Ill."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Usama","family":"Bukhari","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Section of Cardiology (M.A.H.B., J.G., U.B., P.T.T., G.K., J.E., E.M.M.) and Department of Pathology (A.N.H.), University of Chicago, Chicago, Ill."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aliya N.","family":"Husain","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Section of Cardiology (M.A.H.B., J.G., U.B., P.T.T., G.K., J.E., E.M.M.) and Department of Pathology (A.N.H.), University of Chicago, Chicago, Ill."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter T.","family":"Toth","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Section of Cardiology (M.A.H.B., J.G., U.B., P.T.T., G.K., J.E., E.M.M.) and Department of Pathology (A.N.H.), University of Chicago, Chicago, Ill."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gene","family":"Kim","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Section of Cardiology (M.A.H.B., J.G., U.B., P.T.T., G.K., J.E., E.M.M.) and Department of Pathology (A.N.H.), University of Chicago, Chicago, Ill."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judy","family":"Earley","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Section of Cardiology (M.A.H.B., J.G., U.B., P.T.T., G.K., J.E., E.M.M.) and Department of Pathology (A.N.H.), University of Chicago, Chicago, Ill."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth M.","family":"McNally","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Section of Cardiology (M.A.H.B., J.G., U.B., P.T.T., G.K., J.E., E.M.M.) and Department of Pathology (A.N.H.), University of Chicago, Chicago, Ill."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.20.5.1262"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.107.732867"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20999"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0306170"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.209486"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200317115"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M509442200"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000131783.74034.97"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2004.8.334"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000147961.09840.fb"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.109.134205"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2005-2559"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1159\/000148646"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000227513.13697.ac"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000070591.21548.69"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M800021200"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.9.2381"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2009.129"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2007.02.002"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2008050514"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0265t.x"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.12.6631-6636.1999"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/43459"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/hh0901.090299"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.105.607333"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80801-6"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI32703"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.2337\/db07-1808"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.109.201103"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.109.212217"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.108.814582"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.atv.0000166600.58468.1b"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0709958105"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00799.2009"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.14.9496"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900373"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0738"}],"container-title":["Arteriosclerosis, Thrombosis, and Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/ATVBAHA.110.217745","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T23:19:34Z","timestamp":1715555974000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/ATVBAHA.110.217745"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,2]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,2]]}},"alternative-id":["10.1161\/ATVBAHA.110.217745"],"URL":"https:\/\/doi.org\/10.1161\/atvbaha.110.217745","relation":{},"ISSN":["1079-5642","1524-4636"],"issn-type":[{"value":"1079-5642","type":"print"},{"value":"1524-4636","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,2]]},"assertion":[{"value":"2010-06-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-10-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-10-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}