{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T06:58:51Z","timestamp":1776754731342,"version":"3.51.2"},"reference-count":46,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"12","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:sec>\n            <jats:title>Objective\u2014<\/jats:title>\n            <jats:p>The pathogenesis of atherosclerosis is associated with the early retention of low-density lipoproteins that are trapped in the extracellular matrix of the arterial intima by interaction with glycosaminoglycan side chains of proteoglycans. Mutant mouse\/human chimeric antibodies of the murine monoclonal antibody P3, which react with N-glycolyl\u2013containing gangliosides and sulfated glycosaminoglycans, were tested for their potentially antiatherogenic properties through the induction of an idiotypic antibody network that may specifically interfere with the binding of low-density lipoproteins to proteoglycan side chains, low-density lipoprotein modification, and foam cell formation.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods and Results\u2014<\/jats:title>\n            <jats:p>\n              Apolipoprotein E\u2013deficient mice fed a high-fat, high-cholesterol diet received 5 to 6 doses of chP3R99 or chP3S98 mutant antibodies, showing high and low reactivity, respectively, against their respective antigens. Both chimeric antibodies elicited an immunodominant anti-idiotypic response in the absence of adjuvant. A striking (40%\u201343%) reduction (\n              <jats:italic>P<\/jats:italic>\n              &lt;0.01) in total lesion areas was observed in 18-week-old mice immunized with chP3R99, but not chP3S98, compared with PBS-treated mice. The antiatherosclerotic effect was associated with increased mice sera reactivity against heparin and sulfated glycosaminoglycans, including chondroitin and dermatan sulfate. In addition, purified IgG from chP3R99-immunized mice blocked the retention of apolipoprotein B\u2013containing lipoproteins within the arterial wall of apolipoprotein E\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              mice.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion\u2014<\/jats:title>\n            <jats:p>The present study supports use of active immunization and the mounting of an idiotypic antibody network response against glycosaminoglycans as a novel approach to target atherosclerosis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/atvbaha.112.300444","type":"journal-article","created":{"date-parts":[[2012,10,20]],"date-time":"2012-10-20T01:21:26Z","timestamp":1350696086000},"page":"2847-2854","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":31,"title":["Induction of Anti-Anti-Idiotype Antibodies Against Sulfated Glycosaminoglycans Reduces Atherosclerosis in Apolipoprotein E\u2013Deficient Mice"],"prefix":"10.1161","volume":"32","author":[{"given":"V\u00edctor","family":"Brito","sequence":"first","affiliation":[{"name":"From the Faculty of Pharmacy, Universit\u00e9 de Montr\u00e9al; Montr\u00e9al, Qu\u00e9bec, Canada (K.M., S.G.P., D.dB., H.O., S.M.); and the Division of Immunobiology, Center of Molecular Immunology, Havana, Cuba (V.B., A.M.V., A.P., Y.S.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katia","family":"Mellal","sequence":"additional","affiliation":[{"name":"From the Faculty of Pharmacy, Universit\u00e9 de Montr\u00e9al; Montr\u00e9al, Qu\u00e9bec, Canada (K.M., S.G.P., D.dB., H.O., S.M.); and the Division of Immunobiology, Center of Molecular Immunology, Havana, Cuba (V.B., A.M.V., A.P., Y.S.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon Giroux","family":"Portelance","sequence":"additional","affiliation":[{"name":"From the Faculty of Pharmacy, Universit\u00e9 de Montr\u00e9al; Montr\u00e9al, Qu\u00e9bec, Canada (K.M., S.G.P., D.dB., H.O., S.M.); and the Division of Immunobiology, Center of Molecular Immunology, Havana, Cuba (V.B., A.M.V., A.P., Y.S.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arlenis","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"From the Faculty of Pharmacy, Universit\u00e9 de Montr\u00e9al; Montr\u00e9al, Qu\u00e9bec, Canada (K.M., S.G.P., D.dB., H.O., S.M.); and the Division of Immunobiology, Center of Molecular Immunology, Havana, Cuba (V.B., A.M.V., A.P., Y.S.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yosdel","family":"Soto","sequence":"additional","affiliation":[{"name":"From the Faculty of Pharmacy, Universit\u00e9 de Montr\u00e9al; Montr\u00e9al, Qu\u00e9bec, Canada (K.M., S.G.P., D.dB., H.O., S.M.); and the Division of Immunobiology, Center of Molecular Immunology, Havana, Cuba (V.B., A.M.V., A.P., Y.S.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis","family":"deBlois","sequence":"additional","affiliation":[{"name":"From the Faculty of Pharmacy, Universit\u00e9 de Montr\u00e9al; Montr\u00e9al, Qu\u00e9bec, Canada (K.M., S.G.P., D.dB., H.O., S.M.); and the Division of Immunobiology, Center of Molecular Immunology, Havana, Cuba (V.B., A.M.V., A.P., Y.S.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huy","family":"Ong","sequence":"additional","affiliation":[{"name":"From the Faculty of Pharmacy, Universit\u00e9 de Montr\u00e9al; Montr\u00e9al, Qu\u00e9bec, Canada (K.M., S.G.P., D.dB., H.O., S.M.); and the Division of Immunobiology, Center of Molecular Immunology, Havana, Cuba (V.B., A.M.V., A.P., Y.S.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvie","family":"Marleau","sequence":"additional","affiliation":[{"name":"From the Faculty of Pharmacy, Universit\u00e9 de Montr\u00e9al; Montr\u00e9al, Qu\u00e9bec, Canada (K.M., S.G.P., D.dB., H.O., S.M.); and the Division of Immunobiology, Center of Molecular Immunology, Havana, Cuba (V.B., A.M.V., A.P., Y.S.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Mar\u00eda","family":"V\u00e1zquez","sequence":"additional","affiliation":[{"name":"From the Faculty of Pharmacy, Universit\u00e9 de Montr\u00e9al; Montr\u00e9al, Qu\u00e9bec, Canada (K.M., S.G.P., D.dB., H.O., S.M.); and the Division of Immunobiology, Center of Molecular Immunology, Havana, Cuba (V.B., A.M.V., A.P., Y.S.)."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.2174\/187152910793743832"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11906-010-0143-1"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2009.07.002"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra043430"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.106.676890"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.15.5.551"},{"key":"e_1_3_4_8_2","first-page":"457","article-title":"Interactions of lipoproteins with proteoglycans.","volume":"171","author":"Williams KJ","year":"2001","unstructured":"Williams KJ. 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