{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T20:22:08Z","timestamp":1789417328021,"version":"build-2803163510"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:sec>\n            <jats:title>Objective\u2014<\/jats:title>\n            <jats:p>Interleukin-8 (IL-8) receptors IL8RA and IL8RB (IL8RA\/B) on neutrophil membranes bind to IL-8 with high affinity and play a critical role in neutrophil recruitment to sites of injury and inflammation. This study tested the hypothesis that administration of rat pulmonary arterial endothelial cells (ECs) overexpressing IL8RA\/B can accelerate the adhesion of ECs to the injured lung and inhibit monocrotaline-induced pulmonary inflammation, arterial thickening and hypertension, and right ventricular hypertrophy.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Approach and Results\u2014<\/jats:title>\n            <jats:p>\n              The treatment groups included 10-week-old ovariectomized Sprague-Dawley rats that received subcutaneous injection of PBS (vehicle), a single injection of monocrotaline (monocrotaline alone, 60 mg\/kg, SC), monocrotaline followed by intravenous transfusion of ECs transduced with the empty adenoviral vector (null-EC), and monocrotaline followed by intravenous transfusion of ECs overexpressing IL8RA\/B (1.5\u00d710\n              <jats:sup>6<\/jats:sup>\n              cells\/rat). Two days or 4 weeks after monocrotaline treatment, endothelial nitric oxide synthase, inducible nitric oxide synthase, cytokine-induced neutrophil chemoattractant-2\u03b2 (IL-8 equivalent in rat), and monocyte chemoattractant protein-1 expression, neutrophil and macrophage infiltration into pulmonary arterioles, and arteriolar and alveolar morphology were measured by histological and immunohistochemical techniques. Proinflammatory cytokine\/chemokine protein levels were measured by Multiplex rat-specific magnetic bead\u2013based sandwich immunoassay in total lung homogenates. Transfusion of ECs overexpressing IL8RA\/B significantly reduced monocrotaline-induced neutrophil infiltration and proinflammatory mediator (IL-8, monocyte chemoattractant protein-1, inducible nitric oxide synthase, cytokine-induced neutrophil chemoattractant, and macrophage inflammatory protein-2) expression in lungs and pulmonary arterioles and alveoli, pulmonary arterial pressure, and pulmonary arterial and right ventricular hypertrophy and remodeling.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions\u2014<\/jats:title>\n            <jats:p>These provocative findings suggest that targeted delivery of ECs overexpressing IL8RA\/B is effective in repairing the injured pulmonary vasculature.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/atvbaha.114.303821","type":"journal-article","created":{"date-parts":[[2014,5,2]],"date-time":"2014-05-02T02:55:19Z","timestamp":1398999319000},"page":"1539-1547","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":17,"title":["Targeted Delivery of Pulmonary Arterial Endothelial Cells Overexpressing Interleukin-8 Receptors Attenuates Monocrotaline-Induced Pulmonary Vascular Remodeling"],"prefix":"10.1161","volume":"34","author":[{"given":"Jinyan","family":"Fu","sequence":"first","affiliation":[{"name":"From the Vascular Biology and Hypertension Program, Division of Cardiovascular Disease, Department of Medicine (J.F., Y.-F.C., X.Z., Y.G., F.G.H., S.O., D.D.X.) and Department of Anesthesiology, University of Alabama at Birmingham (J.R.C.); Department of Biochemistry and the Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Xinjiang, China (J.F.); and Section of Cardiology, Birmingham Veteran\u2019s Administration Medical Center, AL (F.G.H.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiu-Fai","family":"Chen","sequence":"additional","affiliation":[{"name":"From the Vascular Biology and Hypertension Program, Division of Cardiovascular Disease, Department of Medicine (J.F., Y.-F.C., X.Z., Y.G., F.G.H., S.O., D.D.X.) and Department of Anesthesiology, University of Alabama at Birmingham (J.R.C.); Department of Biochemistry and the Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Xinjiang, China (J.F.); and Section of Cardiology, Birmingham Veteran\u2019s Administration Medical Center, AL (F.G.H.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangmin","family":"Zhao","sequence":"additional","affiliation":[{"name":"From the Vascular Biology and Hypertension Program, Division of Cardiovascular Disease, Department of Medicine (J.F., Y.-F.C., X.Z., Y.G., F.G.H., S.O., D.D.X.) and Department of Anesthesiology, University of Alabama at Birmingham (J.R.C.); Department of Biochemistry and the Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Xinjiang, China (J.F.); and Section of Cardiology, Birmingham Veteran\u2019s Administration Medical Center, AL (F.G.H.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judy R.","family":"Creighton","sequence":"additional","affiliation":[{"name":"From the Vascular Biology and Hypertension Program, Division of Cardiovascular Disease, Department of Medicine (J.F., Y.-F.C., X.Z., Y.G., F.G.H., S.O., D.D.X.) and Department of Anesthesiology, University of Alabama at Birmingham (J.R.C.); Department of Biochemistry and the Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Xinjiang, China (J.F.); and Section of Cardiology, Birmingham Veteran\u2019s Administration Medical Center, AL (F.G.H.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Guo","sequence":"additional","affiliation":[{"name":"From the Vascular Biology and Hypertension Program, Division of Cardiovascular Disease, Department of Medicine (J.F., Y.-F.C., X.Z., Y.G., F.G.H., S.O., D.D.X.) and Department of Anesthesiology, University of Alabama at Birmingham (J.R.C.); Department of Biochemistry and the Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Xinjiang, China (J.F.); and Section of Cardiology, Birmingham Veteran\u2019s Administration Medical Center, AL (F.G.H.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fadi G.","family":"Hage","sequence":"additional","affiliation":[{"name":"From the Vascular Biology and Hypertension Program, Division of Cardiovascular Disease, Department of Medicine (J.F., Y.-F.C., X.Z., Y.G., F.G.H., S.O., D.D.X.) and Department of Anesthesiology, University of Alabama at Birmingham (J.R.C.); Department of Biochemistry and the Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Xinjiang, China (J.F.); and Section of Cardiology, Birmingham Veteran\u2019s Administration Medical Center, AL (F.G.H.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne","family":"Oparil","sequence":"additional","affiliation":[{"name":"From the Vascular Biology and Hypertension Program, Division of Cardiovascular Disease, Department of Medicine (J.F., Y.-F.C., X.Z., Y.G., F.G.H., S.O., D.D.X.) and Department of Anesthesiology, University of Alabama at Birmingham (J.R.C.); Department of Biochemistry and the Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Xinjiang, China (J.F.); and Section of Cardiology, Birmingham Veteran\u2019s Administration Medical Center, AL (F.G.H.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisy D.","family":"Xing","sequence":"additional","affiliation":[{"name":"From the Vascular Biology and Hypertension Program, Division of Cardiovascular Disease, Department of Medicine (J.F., Y.-F.C., X.Z., Y.G., F.G.H., S.O., D.D.X.) and Department of Anesthesiology, University of Alabama at Birmingham (J.R.C.); Department of Biochemistry and the Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Xinjiang, China (J.F.); and Section of Cardiology, Birmingham Veteran\u2019s Administration Medical Center, AL (F.G.H.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0208125"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2011.05.073"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.111.078436"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00571.2012"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2003.11.006"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00979.2001"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.2174\/187152511797037501"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00046612"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.3109\/10408449209146311"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1517\/13543781003727099"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1506"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11906-013-0394-8"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.112.114173"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1159\/000350177"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00380-013-0395-1"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.3727\/096368913X667736"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.113.003139"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.109.202028"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.2013.765863"},{"key":"e_1_3_4_21_2","first-page":"H149","article-title":"Changes in pulmonary structure and function induced by monocrotaline intoxication.","volume":"240","author":"Ghodsi F","year":"1981","unstructured":"Ghodsi F, Will JA. Changes in pulmonary structure and function induced by monocrotaline intoxication. Am J Physiol. 1981;240:H149\u2013H155.","journal-title":"Am J Physiol"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00212.2011"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(99)80022-7"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M607705200"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.110.209296"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.090480"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2011.06.028"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1517\/14712590903002047"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2007.12.025"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(02)00823-7"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1159\/000346921"}],"container-title":["Arteriosclerosis, Thrombosis, and Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/ATVBAHA.114.303821","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T13:37:07Z","timestamp":1718804227000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/ATVBAHA.114.303821"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7]]},"references-count":30,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2014,7]]}},"alternative-id":["10.1161\/ATVBAHA.114.303821"],"URL":"https:\/\/doi.org\/10.1161\/atvbaha.114.303821","relation":{},"ISSN":["1079-5642","1524-4636"],"issn-type":[{"value":"1079-5642","type":"print"},{"value":"1524-4636","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7]]},"assertion":[{"value":"2013-11-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-04-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}