{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:58:19Z","timestamp":1791917899895,"version":"4.3.4"},"reference-count":31,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec>\n            <jats:title>Objective\u2014<\/jats:title>\n            <jats:p>\n              In resistance arteries, there is an emerging view that smooth muscle Ca\n              <jats:sub>V<\/jats:sub>\n              3.2 channels restrain arterial constriction through a feedback response involving the large-conductance Ca\n              <jats:sup>2+<\/jats:sup>\n              -activated K\n              <jats:sup>+<\/jats:sup>\n              channel (BK\n              <jats:sub>Ca<\/jats:sub>\n              ). Here, we used wild-type and Ca\n              <jats:sub>V<\/jats:sub>\n              3.2 knockout (Ca\n              <jats:sub>V<\/jats:sub>\n              3.2\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              ) mice to definitively test whether Ca\n              <jats:sub>V<\/jats:sub>\n              3.2 moderates myogenic tone in mesenteric arteries via the Ca\n              <jats:sub>V<\/jats:sub>\n              3.2-ryanodine receptor-BK\n              <jats:sub>Ca<\/jats:sub>\n              axis and whether this regulatory mechanism influences blood pressure regulation.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Approach and Results\u2014<\/jats:title>\n            <jats:p>\n              Using pressurized vessel myography, Ca\n              <jats:sub>V<\/jats:sub>\n              3.2\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              mesenteric arteries displayed enhanced myogenic constriction to pressure but similar K\n              <jats:sup>+<\/jats:sup>\n              -induced vasoconstriction compared with wild-type C57BL\/6 arteries. Electrophysiological and myography experiments subsequently confirmed the inability of micromolar Ni\n              <jats:sup>2+<\/jats:sup>\n              , a Ca\n              <jats:sub>V<\/jats:sub>\n              3.2 blocker, to either constrict arteries or suppress T-type currents in Ca\n              <jats:sub>V<\/jats:sub>\n              3.2\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              smooth muscle cells. The frequency of BK\n              <jats:sub>Ca<\/jats:sub>\n              -induced spontaneous transient outward K\n              <jats:sup>+<\/jats:sup>\n              currents dropped in wild-type but not in knockout arterial smooth muscle cells upon the pharmacological suppression of Ca\n              <jats:sub>V<\/jats:sub>\n              3.2 channel. Line scan analysis performed on en face arteries loaded with Fluo-4 revealed the presence of Ca\n              <jats:sup>2+<\/jats:sup>\n              sparks in all arteries, with the subsequent application of Ni\n              <jats:sup>2+<\/jats:sup>\n              only affecting wild-type arteries. Although Ca\n              <jats:sub>V<\/jats:sub>\n              3.2 channel moderated myogenic constriction of resistance arteries, the blood pressure measurements of Ca\n              <jats:sub>V<\/jats:sub>\n              3.2\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              and wild-type animals were similar.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions\u2014<\/jats:title>\n            <jats:p>\n              Overall, our findings establish a negative feedback mechanism of the myogenic response in which Ca\n              <jats:sub>V<\/jats:sub>\n              3.2 channel modulates downstream ryanodine receptor-BK\n              <jats:sub>Ca<\/jats:sub>\n              to hyperpolarize and relax arteries.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/atvbaha.115.305736","type":"journal-article","created":{"date-parts":[[2015,6,12]],"date-time":"2015-06-12T03:17:31Z","timestamp":1434079051000},"page":"1843-1851","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":49,"title":["Genetic Ablation of Ca\n            <sub>V<\/sub>\n            3.2 Channels Enhances the Arterial Myogenic Response by Modulating the RyR-BK\n            <sub>Ca<\/sub>\n            Axis"],"prefix":"10.1161","volume":"35","author":[{"given":"Osama F.","family":"Harraz","sequence":"first","affiliation":[{"name":"From the Department of Physiology and Pharmacology, Hotchkiss Brain and Libin Cardiovascular Institutes, University of Calgary, Calgary, Alberta, Canada (O.F.H., S.E.B., A.Z., D.G.W.); Department of Pharmacology and Toxicology, Alexandria University, Alexandria, Egypt (O.F.H.); Department of Basic Sciences, Division of Pharmacology, Loma Linda University, CA (M.R., S.M.W.); Department of Pharmacology, University of California, Davis (J.L.P.); and Department of Physiology and Pharmacology, University..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne E.","family":"Brett","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Pharmacology, Hotchkiss Brain and Libin Cardiovascular Institutes, University of Calgary, Calgary, Alberta, Canada (O.F.H., S.E.B., A.Z., D.G.W.); Department of Pharmacology and Toxicology, Alexandria University, Alexandria, Egypt (O.F.H.); Department of Basic Sciences, Division of Pharmacology, Loma Linda University, CA (M.R., S.M.W.); Department of Pharmacology, University of California, Davis (J.L.P.); and Department of Physiology and Pharmacology, University..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil","family":"Zechariah","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Pharmacology, Hotchkiss Brain and Libin Cardiovascular Institutes, University of Calgary, Calgary, Alberta, Canada (O.F.H., S.E.B., A.Z., D.G.W.); Department of Pharmacology and Toxicology, Alexandria University, Alexandria, Egypt (O.F.H.); Department of Basic Sciences, Division of Pharmacology, Loma Linda University, CA (M.R., S.M.W.); Department of Pharmacology, University of California, Davis (J.L.P.); and Department of Physiology and Pharmacology, University..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Romero","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Pharmacology, Hotchkiss Brain and Libin Cardiovascular Institutes, University of Calgary, Calgary, Alberta, Canada (O.F.H., S.E.B., A.Z., D.G.W.); Department of Pharmacology and Toxicology, Alexandria University, Alexandria, Egypt (O.F.H.); Department of Basic Sciences, Division of Pharmacology, Loma Linda University, CA (M.R., S.M.W.); Department of Pharmacology, University of California, Davis (J.L.P.); and Department of Physiology and Pharmacology, University..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose L.","family":"Puglisi","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Pharmacology, Hotchkiss Brain and Libin Cardiovascular Institutes, University of Calgary, Calgary, Alberta, Canada (O.F.H., S.E.B., A.Z., D.G.W.); Department of Pharmacology and Toxicology, Alexandria University, Alexandria, Egypt (O.F.H.); Department of Basic Sciences, Division of Pharmacology, Loma Linda University, CA (M.R., S.M.W.); Department of Pharmacology, University of California, Davis (J.L.P.); and Department of Physiology and Pharmacology, University..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean M.","family":"Wilson","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Pharmacology, Hotchkiss Brain and Libin Cardiovascular Institutes, University of Calgary, Calgary, Alberta, Canada (O.F.H., S.E.B., A.Z., D.G.W.); Department of Pharmacology and Toxicology, Alexandria University, Alexandria, Egypt (O.F.H.); Department of Basic Sciences, Division of Pharmacology, Loma Linda University, CA (M.R., S.M.W.); Department of Pharmacology, University of California, Davis (J.L.P.); and Department of Physiology and Pharmacology, University..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald G.","family":"Welsh","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Pharmacology, Hotchkiss Brain and Libin Cardiovascular Institutes, University of Calgary, Calgary, Alberta, Canada (O.F.H., S.E.B., A.Z., D.G.W.); Department of Pharmacology and Toxicology, Alexandria University, Alexandria, Egypt (O.F.H.); Department of Basic Sciences, Division of Pharmacology, Loma Linda University, CA (M.R., S.M.W.); Department of Pharmacology, University of California, Davis (J.L.P.); and Department of Physiology and Pharmacology, University..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.56.2.205"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.66.5.1445"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.199br.x"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000024417.79275.23"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn1779"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1902.sp000911"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2010.11"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00476.2012"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12066"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.304056"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(99)77134-1"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1089268"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900059"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.128363"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvt043"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.113.2.229"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.184051"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00016.2014"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2011.02.024"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.191544"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00686.2006"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-009-0782-8"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1373909"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5236.633"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-09-03404.2002"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2124"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1115024109"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061844"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.88.9"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2009.169920"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201511361"}],"container-title":["Arteriosclerosis, Thrombosis, and Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/ATVBAHA.115.305736","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T22:45:15Z","timestamp":1715553915000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/ATVBAHA.115.305736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8]]},"references-count":31,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1161\/ATVBAHA.115.305736"],"URL":"https:\/\/doi.org\/10.1161\/atvbaha.115.305736","relation":{},"ISSN":["1079-5642","1524-4636"],"issn-type":[{"value":"1079-5642","type":"print"},{"value":"1524-4636","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8]]},"assertion":[{"value":"2015-04-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-05-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-06-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}