{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:53:40Z","timestamp":1787018020137,"version":"build-2736575974"},"reference-count":47,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:sec>\n                    <jats:title>Objective\u2014<\/jats:title>\n                    <jats:p>Vascular smooth muscle cell (SMC) migration is regulated by cytoskeletal remodeling as well as by certain transient receptor potential (TRP) channels, nonselective cation channels that modulate calcium influx. Proper function of multiple subfamily C TRP (TRPC) channels requires the scaffolding protein Homer 1, which associates with the actin-binding protein Drebrin. We found that SMC Drebrin expression is upregulated in atherosclerosis and in response to injury and investigated whether Drebrin inhibits SMC activation, either through regulation of TRP channel function via Homer or through a direct effect on the actin cytoskeleton.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Approach and Results\u2014<\/jats:title>\n                    <jats:p>\n                      Wild-type (WT) and congenic\n                      <jats:italic>Dbn<\/jats:italic>\n                      <jats:sup>\u2212\/+<\/jats:sup>\n                      mice were subjected to wire-mediated carotid endothelial denudation. Subsequent neointimal hyperplasia was 2.4\u00b10.3-fold greater in\n                      <jats:italic>Dbn<\/jats:italic>\n                      <jats:sup>\u2212\/+<\/jats:sup>\n                      than in WT mice. Levels of globular actin were equivalent in\n                      <jats:italic>Dbn<\/jats:italic>\n                      <jats:sup>\u2212\/+<\/jats:sup>\n                      and WT SMCs, but there was a 2.4\u00b10.5-fold decrease in filamentous actin in\n                      <jats:italic>Dbn<\/jats:italic>\n                      <jats:sup>\u2212\/+<\/jats:sup>\n                      SMCs compared with WT. Filamentous actin was restored to WT levels in\n                      <jats:italic>Dbn<\/jats:italic>\n                      <jats:sup>\u2212\/+<\/jats:sup>\n                      SMCs by adenoviral-mediated rescue expression of Drebrin. Compared with WT SMCs,\n                      <jats:italic>Dbn<\/jats:italic>\n                      <jats:sup>\u2212\/+<\/jats:sup>\n                      SMCs exhibited increased TRP channel activity in response to platelet-derived growth factor, increased migration assessed in Boyden chambers, and increased proliferation. Enhanced TRP channel activity and migration in\n                      <jats:italic>Dbn<\/jats:italic>\n                      <jats:sup>\u2212\/+<\/jats:sup>\n                      SMCs were normalized to WT levels by rescue expression of not only WT Drebrin but also a mutant Drebrin isoform that binds actin but fails to bind Homer.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions\u2014<\/jats:title>\n                    <jats:p>Drebrin reduces SMC activation through its interaction with the actin cytoskeleton but independently of its interaction with Homer scaffolds.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/atvbaha.115.306140","type":"journal-article","created":{"date-parts":[[2016,3,24]],"date-time":"2016-03-24T20:21:42Z","timestamp":1458850902000},"page":"984-993","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":20,"title":["The Actin-Binding Protein Drebrin Inhibits Neointimal Hyperplasia"],"prefix":"10.1161","volume":"36","author":[{"given":"Jonathan A.","family":"Stiber","sequence":"first","affiliation":[{"name":"From the Department of Medicine, Duke University Medical Center, Durham, NC (J.A.S., J.-H.W., L.Z., I.N., Z.-S.Z., V.G.B., L.B., P.B.R., N.J.F.); Department of Pathology, Duke University Medical Center, Durham, NC (R.C.B.); and MRC Centre for Developmental Neurobiology, King\u2019s College, London, UK (P.R.G.-W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao-Hui","family":"Wu","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Duke University Medical Center, Durham, NC (J.A.S., J.-H.W., L.Z., I.N., Z.-S.Z., V.G.B., L.B., P.B.R., N.J.F.); Department of Pathology, Duke University Medical Center, Durham, NC (R.C.B.); and MRC Centre for Developmental Neurobiology, King\u2019s College, London, UK (P.R.G.-W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Duke University Medical Center, Durham, NC (J.A.S., J.-H.W., L.Z., I.N., Z.-S.Z., V.G.B., L.B., P.B.R., N.J.F.); Department of Pathology, Duke University Medical Center, Durham, NC (R.C.B.); and MRC Centre for Developmental Neurobiology, King\u2019s College, London, UK (P.R.G.-W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Nepliouev","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Duke University Medical Center, Durham, NC (J.A.S., J.-H.W., L.Z., I.N., Z.-S.Z., V.G.B., L.B., P.B.R., N.J.F.); Department of Pathology, Duke University Medical Center, Durham, NC (R.C.B.); and MRC Centre for Developmental Neurobiology, King\u2019s College, London, UK (P.R.G.-W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhu-Shan","family":"Zhang","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Duke University Medical Center, Durham, NC (J.A.S., J.-H.W., L.Z., I.N., Z.-S.Z., V.G.B., L.B., P.B.R., N.J.F.); Department of Pathology, Duke University Medical Center, Durham, NC (R.C.B.); and MRC Centre for Developmental Neurobiology, King\u2019s College, London, UK (P.R.G.-W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria G.","family":"Bryson","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Duke University Medical Center, Durham, NC (J.A.S., J.-H.W., L.Z., I.N., Z.-S.Z., V.G.B., L.B., P.B.R., N.J.F.); Department of Pathology, Duke University Medical Center, Durham, NC (R.C.B.); and MRC Centre for Developmental Neurobiology, King\u2019s College, London, UK (P.R.G.-W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leigh","family":"Brian","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Duke University Medical Center, Durham, NC (J.A.S., J.-H.W., L.Z., I.N., Z.-S.Z., V.G.B., L.B., P.B.R., N.J.F.); Department of Pathology, Duke University Medical Center, Durham, NC (R.C.B.); and MRC Centre for Developmental Neurobiology, King\u2019s College, London, UK (P.R.G.-W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rex C.","family":"Bentley","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Duke University Medical Center, Durham, NC (J.A.S., J.-H.W., L.Z., I.N., Z.-S.Z., V.G.B., L.B., P.B.R., N.J.F.); Department of Pathology, Duke University Medical Center, Durham, NC (R.C.B.); and MRC Centre for Developmental Neurobiology, King\u2019s College, London, UK (P.R.G.-W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip R.","family":"Gordon-Weeks","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Duke University Medical Center, Durham, NC (J.A.S., J.-H.W., L.Z., I.N., Z.-S.Z., V.G.B., L.B., P.B.R., N.J.F.); Department of Pathology, Duke University Medical Center, Durham, NC (R.C.B.); and MRC Centre for Developmental Neurobiology, King\u2019s College, London, UK (P.R.G.-W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul B.","family":"Rosenberg","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Duke University Medical Center, Durham, NC (J.A.S., J.-H.W., L.Z., I.N., Z.-S.Z., V.G.B., L.B., P.B.R., N.J.F.); Department of Pathology, Duke University Medical Center, Durham, NC (R.C.B.); and MRC Centre for Developmental Neurobiology, King\u2019s College, London, UK (P.R.G.-W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil J.","family":"Freedman","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Duke University Medical Center, Durham, NC (J.A.S., J.-H.W., L.Z., I.N., Z.-S.Z., V.G.B., L.B., P.B.R., N.J.F.); Department of Pathology, Duke University Medical Center, Durham, NC (R.C.B.); and MRC Centre for Developmental Neurobiology, King\u2019s College, London, UK (P.R.G.-W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_5_2_2","first-page":"71","article-title":"Molecular cloning of a cDNA for the developmentally regulated brain protein, drebrin.","volume":"464","author":"Shirao T","year":"1988","unstructured":"Shirao T, Kojima N, Kato Y, Obata K. 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