{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T01:44:59Z","timestamp":1793065499129,"version":"4.5.0"},"reference-count":33,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec>\n            <jats:title>Objective\u2014<\/jats:title>\n            <jats:p>von Willebrand factor (VWF), which is synthesized in endothelial cells and megakaryocytes, is known to worsen stroke outcome. In vitro studies suggest that platelet-derived VWF (Plt-VWF) is biochemically different from the endothelial cell\u2013derived VWF (EC-VWF). However, little is known about relative contribution of different pools of VWF in stroke.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Approach and Results\u2014<\/jats:title>\n            <jats:p>\n              Using bone marrow transplantation, we generated chimeric Plt-VWF mice, Plt-VWF mice that lack ADAMTS13 in platelets and plasma (Plt-VWF\/\n              <jats:italic>Adamts<\/jats:italic>\n              13\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              ), and EC-VWF mice to determine relative contribution of different pools of VWF in stroke. In brain ischemia\/reperfusion injury model, we found that infarct size and postischemic intracerebral thrombo-inflammation (fibrin(ogen) deposition, neutrophil infiltration, interleukin-1\u03b2, and tumor necrosis factor-\u03b1 levels) within lesions were comparable between EC-VWF and wild-type mice. Infarct size and postischemic thrombo-inflammation were comparable between Plt-VWF and Plt-VWF\/\n              <jats:italic>Adamts<\/jats:italic>\n              13\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              mice, but decreased compared with EC-VWF and wild-type mice (\n              <jats:italic>P<\/jats:italic>\n              &lt;0.05) and increased compared with\n              <jats:italic>Vwf<\/jats:italic>\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              mice (\n              <jats:italic>P<\/jats:italic>\n              &lt;0.05). Susceptibility to FeCl\n              <jats:sub>3<\/jats:sub>\n              injury\u2013induced carotid artery thrombosis was comparable between wild-type and EC-VWF mice, whereas Plt-VWF and Plt-VWF\/\n              <jats:italic>Adamts<\/jats:italic>\n              13\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              mice exhibited defective thrombosis. Although most of the injured vessels did not occlude, slope over time showed that thrombus growth rate was increased in both Plt-VWF and Plt-VWF\/\n              <jats:italic>Adamts<\/jats:italic>\n              13\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              mice compared with\n              <jats:italic>Vwf<\/jats:italic>\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              mice (\n              <jats:italic>P<\/jats:italic>\n              &lt;0.05), but decreased compared with wild-type or EC-VWF mice.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions\u2014<\/jats:title>\n            <jats:p>Plt-VWF, either in presence or absence of ADAMTS13, partially contributes to VWF-dependent injury and postischemic thrombo-inflammation after stroke. EC-VWF is the major determinant that mediates VWF-dependent ischemic stroke by promoting postischemic thrombo-inflammation.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/atvbaha.116.307660","type":"journal-article","created":{"date-parts":[[2016,7,22]],"date-time":"2016-07-22T02:54:06Z","timestamp":1469156046000},"page":"1829-1837","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":61,"title":["Endothelial Cell\u2013Derived von Willebrand Factor Is the Major Determinant That Mediates von Willebrand Factor\u2013Dependent Acute Ischemic Stroke by Promoting Postischemic Thrombo-Inflammation"],"prefix":"10.1161","volume":"36","author":[{"given":"Nirav","family":"Dhanesha","sequence":"first","affiliation":[{"name":"From the Department of Internal Medicine (N.D., P.P., P.D., I.K., M.K.N., A.K.C.) and Stead Family Department of Pediatrics (M.J.P., J.M.S.), University of Iowa, Iowa City."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prem","family":"Prakash","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine (N.D., P.P., P.D., I.K., M.K.N., A.K.C.) and Stead Family Department of Pediatrics (M.J.P., J.M.S.), University of Iowa, Iowa City."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prakash","family":"Doddapattar","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine (N.D., P.P., P.D., I.K., M.K.N., A.K.C.) and Stead Family Department of Pediatrics (M.J.P., J.M.S.), University of Iowa, Iowa City."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ira","family":"Khanna","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine (N.D., P.P., P.D., I.K., M.K.N., A.K.C.) and Stead Family Department of Pediatrics (M.J.P., J.M.S.), University of Iowa, Iowa City."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly J.","family":"Pollpeter","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine (N.D., P.P., P.D., I.K., M.K.N., A.K.C.) and Stead Family Department of Pediatrics (M.J.P., J.M.S.), University of Iowa, Iowa City."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manasa K.","family":"Nayak","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine (N.D., P.P., P.D., I.K., M.K.N., A.K.C.) and Stead Family Department of Pediatrics (M.J.P., J.M.S.), University of Iowa, Iowa City."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice M.","family":"Staber","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine (N.D., P.P., P.D., I.K., M.K.N., A.K.C.) and Stead Family Department of Pediatrics (M.J.P., J.M.S.), University of Iowa, Iowa City."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil K.","family":"Chauhan","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine (N.D., P.P., P.D., I.K., M.K.N., A.K.C.) and Stead Family Department of Pediatrics (M.J.P., J.M.S.), University of Iowa, Iowa City."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.24.8752"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.95.1.355"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-05-1401"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-061813-013241"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1991.tb04528.x"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112064"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.16.9524"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI9896"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-05-287623"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-08-595686"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-01-264507"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-06-440255"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-06-439935"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.5.1424"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12456"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-03-213264"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-09-180695"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.110.208918"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-05-1519"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-04-496851"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V60.5.1132.1132"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2005.01561.x"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1994.tb05077.x"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.7.2428"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-03-632901"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2010.05730.x"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2012.04822.x"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.7.2455"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI7569"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2012.04797.x"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V69.5.1531.1531"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2001-11-0060"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V66.4.796.796"}],"container-title":["Arteriosclerosis, Thrombosis, and Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/ATVBAHA.116.307660","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T23:01:13Z","timestamp":1715554873000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/ATVBAHA.116.307660"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9]]},"references-count":33,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["10.1161\/ATVBAHA.116.307660"],"URL":"https:\/\/doi.org\/10.1161\/atvbaha.116.307660","relation":{},"ISSN":["1079-5642","1524-4636"],"issn-type":[{"value":"1079-5642","type":"print"},{"value":"1524-4636","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9]]},"assertion":[{"value":"2016-04-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-07-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-07-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}