{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T23:55:32Z","timestamp":1789170932960,"version":"build-2803163510"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:sec><jats:title>Objective\u2014<\/jats:title><jats:p>In renal arteries, inhibitors of G protein \u03b2\u03b3 subunits (G\u03b2\u03b3) reduce Kv7 activity and inhibit Kv7-dependent receptor-mediated vasorelaxations. However, the mechanisms underlying receptor-mediated relaxation are artery specific. Consequently, the aim of this study was to ascertain the role of G\u03b2\u03b3 in Kv7-dependent vasorelaxations of the rat vasculature.<\/jats:p><\/jats:sec><jats:sec><jats:title>Approach and Results\u2014<\/jats:title><jats:p>Isometric tension recording was performed in isolated rat renal, mesenteric, and cerebral arteries to study isoproterenol and calcitonin gene-related peptide relaxations. Kv7.4 was knocked down via morpholino transfection while inhibition of G\u03b2\u03b3 was investigated with gallein and M119K. Proximity ligation assay was performed on isolated myocytes to study the association between Kv7.4 and G protein \u03b2 subunits or signaling intermediaries. Isoproterenol or calcitonin gene-related peptide\u2013induced relaxations were attenuated by Kv7.4 knockdown in all arteries studied. Inhibition of G\u03b2\u03b3 with gallein or M119K had no effect on isoproterenol-mediated relaxations in mesenteric artery but had a marked effect on calcitonin gene-related peptide\u2013induced responses in mesenteric artery and cerebral artery and isoproterenol responses in renal artery. Isoproterenol increased association with Kv7.4 and Rap1a in mesenteric artery which were not sensitive to gallein, whereas in renal artery, isoproterenol increased Kv7.4-AKAP (A-kinase anchoring protein) associations in a gallein-sensitive manner.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions\u2014<\/jats:title><jats:p>The G\u03b2\u03b3-Kv7 relationship differs between vessels and is an essential requirement for AKAP, but not Rap\u2013mediated regulation of the channel.<\/jats:p><\/jats:sec>","DOI":"10.1161\/atvbaha.118.311360","type":"journal-article","created":{"date-parts":[[2018,7,12]],"date-time":"2018-07-12T09:06:53Z","timestamp":1531386413000},"page":"2091-2102","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":29,"title":["Investigating the Role of G Protein \u03b2\u03b3 in Kv7-Dependent Relaxations of the Rat Vasculature"],"prefix":"10.1161","volume":"38","author":[{"given":"Jennifer B.","family":"Stott","sequence":"first","affiliation":[{"name":"From the Vascular Biology Research Centre, Institute of Molecular and Clinical Sciences, St George\u2019s University of London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Barrese","sequence":"additional","affiliation":[{"name":"From the Vascular Biology Research Centre, Institute of Molecular and Clinical Sciences, St George\u2019s University of London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malavika","family":"Suresh","sequence":"additional","affiliation":[{"name":"From the Vascular Biology Research Centre, Institute of Molecular and Clinical Sciences, St George\u2019s University of London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirou","family":"Masoodi","sequence":"additional","affiliation":[{"name":"From the Vascular Biology Research Centre, Institute of Molecular and Clinical Sciences, St George\u2019s University of London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iain A.","family":"Greenwood","sequence":"additional","affiliation":[{"name":"From the Vascular Biology Research Centre, Institute of Molecular and Clinical Sciences, St George\u2019s University of London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2016.05.002"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2013.12.003"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.114.303405"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.113.01244"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.114.04373"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvv020"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/micc.12183"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.111.187427"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.116.308517"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.111.032136"},{"key":"e_1_3_5_12_2","doi-asserted-by":"crossref","unstructured":"Carr G Barrese V Stott JB Povstyan OV Jepps TA Figueiredo HB Zheng D Jamshidi Y Greenwood IA. 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