{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:16:32Z","timestamp":1790514992001,"version":"4.1.0"},"reference-count":68,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:sec>\n            <jats:title>Objective\u2014<\/jats:title>\n            <jats:p>\n              Leukocyte flux contributes to thrombus formation in deep veins under pathological conditions, but mechanisms that inhibit venous thrombosis are incompletely understood. Ectonucleotide di(tri)phosphohydrolase 1 (\n              <jats:italic>ENTPD1<\/jats:italic>\n              or\n              <jats:italic>Cd39<\/jats:italic>\n              ), an ectoenzyme that catabolizes extracellular adenine nucleotides, is embedded on the surface of endothelial cells and leukocytes. We hypothesized that under venous stasis conditions, CD39 regulates inflammation at the vein:blood interface in a murine model of deep vein thrombosis.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Approach and Results\u2014<\/jats:title>\n            <jats:p>\n              CD39-null mice developed significantly larger venous thrombi under venous stasis, with more leukocyte recruitment compared with wild-type mice. Gene expression profiling of wild-type and\n              <jats:italic>Cd39<\/jats:italic>\n              -null mice revealed 76 differentially expressed inflammatory genes that were significantly upregulated in\n              <jats:italic>Cd39<\/jats:italic>\n              -deleted mice after venous thrombosis, and validation experiments confirmed high expression of several key inflammatory mediators. P-selectin, known to have proximal involvement in venous inflammatory and thrombotic events, was upregulated in\n              <jats:italic>Cd39<\/jats:italic>\n              -null mice. Inferior vena caval ligation resulted in thrombosis and a corresponding increase in both P-selectin and VWF (von Willebrand Factor) levels which were strikingly higher in mice lacking the\n              <jats:italic>Cd39<\/jats:italic>\n              gene. These mice also manifest an increase in circulating platelet-leukocyte heteroaggregates suggesting heterotypic crosstalk between coagulation and inflammatory systems, which is amplified in the absence of CD39.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions\u2014<\/jats:title>\n            <jats:p>These data suggest that CD39 mitigates the venous thromboinflammatory response to flow interruption.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/atvbaha.119.312407","type":"journal-article","created":{"date-parts":[[2019,2,28]],"date-time":"2019-02-28T14:40:00Z","timestamp":1551364800000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":22,"title":["Tuning the Thromboinflammatory Response to Venous Flow Interruption by the Ectonucleotidase CD39"],"prefix":"10.1161","volume":"39","author":[{"given":"Anuli 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