{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T20:52:24Z","timestamp":1792270344263,"version":"4.4.0"},"reference-count":48,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>microRNAs are master regulators of gene expression with essential roles in virtually all biological processes. miR-217 has been associated with aging and cellular senescence, but its role in vascular disease is not understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Approach and Results:<\/jats:title>\n                    <jats:p>\n                      We have used an inducible endothelium-specific knock-in mouse model to address the role of miR-217 in vascular function and atherosclerosis. miR-217 reduced NO production and promoted endothelial dysfunction, increased blood pressure, and exacerbated atherosclerosis in proatherogenic apoE\n                      <jats:sup>\u2212\/<\/jats:sup>\n                      <jats:sup>\u2212<\/jats:sup>\n                      mice. Moreover, increased endothelial miR-217 expression led to the development of coronary artery disease and altered left ventricular heart function, inducing diastolic and systolic dysfunction. Conversely, inhibition of endogenous vascular miR-217 in apoE\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice improved vascular contractility and diminished atherosclerosis. Transcriptome analysis revealed that miR-217 regulates an endothelial signaling hub and downregulates a network of eNOS (endothelial NO synthase) activators, including VEGF (vascular endothelial growth factor) and apelin receptor pathways, resulting in diminished eNOS expression. Further analysis revealed that human plasma miR-217 is a biomarker of vascular aging and cardiovascular risk.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Our results highlight the therapeutic potential of miR-217 inhibitors in aging-related cardiovascular disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/atvbaha.120.314333","type":"journal-article","created":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T05:00:22Z","timestamp":1598504422000},"page":"2408-2424","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":112,"title":["Aging-Associated miR-217 Aggravates Atherosclerosis and Promotes Cardiovascular Dysfunction"],"prefix":"10.1161","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3785-5868","authenticated-orcid":true,"given":"Virginia G.","family":"de Y\u00e9benes","sequence":"first","affiliation":[{"name":"Department of Vascular Physiopathology, B Lymphocyte Biology Lab (V.G.d.Y., I.M.-F., S.M.M., F.B., A.R.R.), Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain."},{"name":"Department of Immunology, Ophthalmology and ENT, Complutense University School of Medicine, 12 de Octubre Health Research Institute, Madrid, Spain (V.G.d.Y.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M.","family":"Briones","sequence":"additional","affiliation":[{"name":"Departamento de Farmacolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Madrid, Instituto de Investigaci\u00f3n Hospital Universitario La Paz, Spain (A.M.B., M.G.-A., M.S.)."},{"name":"CIBER de Enfermedades Cardiovasculares, Spain (A.M.B., M.G.-A., M.S., J.M.R.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inmaculada","family":"Martos-Folgado","sequence":"additional","affiliation":[{"name":"Department of Vascular Physiopathology, B Lymphocyte Biology Lab (V.G.d.Y., I.M.-F., S.M.M., F.B., A.R.R.), Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia M.","family":"Mur","sequence":"additional","affiliation":[{"name":"Department of Vascular Physiopathology, B Lymphocyte Biology Lab (V.G.d.Y., I.M.-F., S.M.M., F.B., A.R.R.), Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Oller","sequence":"additional","affiliation":[{"name":"Gene Regulation in Cardiovascular Remodelling and Inflammation Lab (J.O., N.M.-B., J.M.R.), Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faiz","family":"Bilal","sequence":"additional","affiliation":[{"name":"Department of Vascular Physiopathology, B Lymphocyte Biology Lab (V.G.d.Y., I.M.-F., S.M.M., F.B., A.R.R.), Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5919-6760","authenticated-orcid":true,"given":"Mar\u00eda","family":"Gonz\u00e1lez-Amor","sequence":"additional","affiliation":[{"name":"Departamento de Farmacolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Madrid, Instituto de Investigaci\u00f3n Hospital Universitario La Paz, Spain (A.M.B., M.G.-A., M.S.)."},{"name":"CIBER de Enfermedades Cardiovasculares, Spain (A.M.B., M.G.-A., M.S., J.M.R.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nerea","family":"M\u00e9ndez-Barbero","sequence":"additional","affiliation":[{"name":"Gene Regulation in Cardiovascular Remodelling and Inflammation Lab (J.O., N.M.-B., J.M.R.), Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6058-5763","authenticated-orcid":true,"given":"Juan Carlos","family":"Silla-Castro","sequence":"additional","affiliation":[{"name":"Bioinformatics Unit, Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain (J.C.S.-C., F.W., F.S.-C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Were","sequence":"additional","affiliation":[{"name":"Bioinformatics Unit, Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain (J.C.S.-C., F.W., F.S.-C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5870-237X","authenticated-orcid":true,"given":"Luis J.","family":"Jim\u00e9nez-Borreguero","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Sanitaria Hospital La Princesa, Madrid, Spain (L.J.J.-B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1tima","family":"S\u00e1nchez-Cabo","sequence":"additional","affiliation":[{"name":"Bioinformatics Unit, Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain (J.C.S.-C., F.W., F.S.-C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9ctor","family":"Bueno","sequence":"additional","affiliation":[{"name":"Department of Cell &amp; Developmental Biology, Multidisciplinary Translational Cardiovascular Research (H.B.), Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Salaices","sequence":"additional","affiliation":[{"name":"Departamento de Farmacolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Madrid, Instituto de Investigaci\u00f3n Hospital Universitario La Paz, Spain (A.M.B., M.G.-A., M.S.)."},{"name":"CIBER de Enfermedades Cardiovasculares, Spain (A.M.B., M.G.-A., M.S., J.M.R.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Miguel","family":"Redondo","sequence":"additional","affiliation":[{"name":"Gene Regulation in Cardiovascular Remodelling and Inflammation Lab (J.O., N.M.-B., J.M.R.), Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain."},{"name":"Departamento de Farmacolog\u00eda, Facultad de Medicina, Universidad Aut\u00f3noma de Madrid, Instituto de Investigaci\u00f3n Hospital Universitario La Paz, Spain (A.M.B., M.G.-A., M.S.)."},{"name":"CIBER de Enfermedades Cardiovasculares, Spain (A.M.B., M.G.-A., M.S., J.M.R.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7539-3844","authenticated-orcid":true,"given":"Almudena R.","family":"Ramiro","sequence":"additional","affiliation":[{"name":"Department of Vascular Physiopathology, B Lymphocyte Biology Lab (V.G.d.Y., I.M.-F., S.M.M., F.B., A.R.R.), Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0b013e3182009701"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.246876"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.306301"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-030212-183737"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.311311"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.153916"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000265065.26744.17"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0804597105"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0000000000000424"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.864629"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehv290"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0704329104"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.602532"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20010187"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-12-543611"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09002"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.3791\/50933"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4266"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2015.10.033"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2017.09.032"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12628"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705549"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20092155"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2013.12.004"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1124\/pr.110.003905"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2009.2469"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.7555\/JBR.30.20150079"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.116.306874"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth1079"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20110503"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2015.01.006"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2007.03.023"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2017.3845"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100162"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehq107"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2015.07.029"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2011.10.001"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2016.12.047"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvn224"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.2009.01964.x"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2014.6158"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2011.11.082"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-19-22"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.15420\/cfr.2016:25:2"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.1136\/heart.84.suppl_1.i15"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1161\/hc2901.091399"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.55.091902.104417"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2018.05.013"}],"container-title":["Arteriosclerosis, Thrombosis, and Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/ATVBAHA.120.314333","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T19:42:58Z","timestamp":1715542978000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/ATVBAHA.120.314333"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10]]},"references-count":48,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["10.1161\/ATVBAHA.120.314333"],"URL":"https:\/\/doi.org\/10.1161\/atvbaha.120.314333","relation":{},"ISSN":["1079-5642","1524-4636"],"issn-type":[{"value":"1079-5642","type":"print"},{"value":"1524-4636","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10]]},"assertion":[{"value":"2020-08-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}