{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:33:33Z","timestamp":1791232413814,"version":"4.1.0"},"reference-count":5,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>Alveolar-capillary endothelial cells can be activated by severe acute respiratory syndrome coronavirus 2 infection leading to cytokine release. This could trigger endothelial dysfunction, pyroptosis, and thrombosis, which are the vascular changes, commonly referred to as coronavirus disease 2019 (COVID-19) endotheliopathy. Thus, this study aimed to identify tissue biomarkers associated with endothelial activation\/dysfunction and the pyroptosis pathway in the lung samples of patients with COVID-19 and to compare them to pandemic influenza A virus H1N1 subtype 2009 and control cases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Approach and Results:<\/jats:title>\n                    <jats:p>Postmortem lung samples (COVID-19 group =6 cases; H1N1 group =10 cases, and control group =11 cases) were analyzed using immunohistochemistry and the following monoclonal primary antibodies: anti-IL (interleukin)-6, anti-TNF (tumor necrosis factor)-\u03b1, anti-ICAM-1 (intercellular adhesion molecule 1), and anticaspase-1. From the result, IL-6, TNF-\u03b1, ICAM-1, and caspase-1 showed higher tissue expression in the COVID-19 group than in the H1N1 and control groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Our results demonstrated endothelial dysfunction and suggested the participation of the pyroptosis pathway in the pulmonary samples. These conditions might lead to systemic thrombotic events that could impair the clinical staff\u2019s efforts to avoid fatal outcomes. One of the health professionals\u2019 goals should be to identify the high risk of thrombosis patients early to block endotheliopathy and its consequences.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/atvbaha.120.314860","type":"journal-article","created":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T11:01:30Z","timestamp":1596798090000},"page":"2404-2407","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":165,"title":["Endothelial Dysfunction and Thrombosis in Patients With COVID-19\u2014Brief Report"],"prefix":"10.1161","volume":"40","author":[{"given":"Seigo","family":"Nagashima","sequence":"first","affiliation":[{"name":"Postgraduate Program of Health Sciences (S.N., A.F.R.d.S.M.), School of Medicine, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2232-8720","authenticated-orcid":true,"given":"Monalisa Castilho","family":"Mendes","sequence":"additional","affiliation":[{"name":"Postgraduate in Biotechnology Applied in Health of Children and Adolescent, Faculdades Pequeno Pr\u00edncipe (FPP), Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe (IPPPP), Curitiba, Paran\u00e1, Brazil (M.C.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6242-7099","authenticated-orcid":true,"given":"Ana Paula","family":"Camargo Martins","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Pathology (A.P.C.M.), School of Medicine, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2529-3869","authenticated-orcid":true,"given":"N\u00edcolas Henrique","family":"Borges","sequence":"additional","affiliation":[{"name":"School of Medicine, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba, Brazil (N.H.B., T.M.G.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1461-7656","authenticated-orcid":true,"given":"Thiago Mateus","family":"Godoy","sequence":"additional","affiliation":[{"name":"School of Medicine, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba, Brazil (N.H.B., T.M.G.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8497-400X","authenticated-orcid":true,"given":"Anna Flavia Ribeiro dos Santos","family":"Miggiolaro","sequence":"additional","affiliation":[{"name":"Postgraduate Program of Health Sciences (S.N., A.F.R.d.S.M.), School of Medicine, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"da Silva Dezid\u00e9rio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0565-3492","authenticated-orcid":true,"given":"Cleber","family":"Machado-Souza","sequence":"additional","affiliation":[{"name":"Faculdades Pequeno Pr\u00edncipe (FPP), Instituto de Pesquisa Pel\u00e9 Pequeno Pr\u00edncipe (IPPPP), Curitiba, Paran\u00e1, Brazil (C.M.-S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0310-7164","authenticated-orcid":true,"given":"Lucia","family":"de Noronha","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Pathology (L.d.N.), School of Medicine, Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1 (PUCPR), Curitiba, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2015432"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2020.04.027"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","unstructured":"Yang M; SSRN Electronic Journal. Cell Pyroptosis a Potential Pathogenic Mechanism of 2019-nCoV Infection. 2020. https:\/\/papers.ssrn.com\/sol3\/papers.cfm?abstract_id=3527420 Accessed April 10 2020.","DOI":"10.2139\/ssrn.3527420"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.835223"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/17.10.1728"}],"container-title":["Arteriosclerosis, Thrombosis, and Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/ATVBAHA.120.314860","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T19:42:35Z","timestamp":1715542955000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/ATVBAHA.120.314860"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10]]},"references-count":5,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["10.1161\/ATVBAHA.120.314860"],"URL":"https:\/\/doi.org\/10.1161\/atvbaha.120.314860","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.06.17.20133124","asserted-by":"object"}]},"ISSN":["1079-5642","1524-4636"],"issn-type":[{"value":"1079-5642","type":"print"},{"value":"1524-4636","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10]]},"assertion":[{"value":"2020-06-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}