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In this brief review, we describe how single-cell technologies have shed a new light on the cellular composition of atherosclerotic plaques, and their response to diet perturbations or genetic manipulation in mouse models of atherosclerosis. We discuss how single-cell RNA sequencing, cellular indexing of transcriptomes and epitopes by sequencing, transposase-accessible chromatin with high-throughput sequencing, and cytometry by time-of-flight platforms have empowered the identification of discrete immune, endothelial, and smooth muscle cell alterations in atherosclerosis progression and regression. Finally, we review how single-cell approaches have allowed mapping the cellular and molecular composition of human atherosclerotic plaques and the discovery of new immune alterations in plaques from patients with stroke.<\/jats:p>","DOI":"10.1161\/atvbaha.121.315849","type":"journal-article","created":{"date-parts":[[2022,2,3]],"date-time":"2022-02-03T10:00:15Z","timestamp":1643882415000},"page":"243-252","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":36,"title":["How Single-Cell Technologies Have Provided New Insights Into Atherosclerosis"],"prefix":"10.1161","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3309-4563","authenticated-orcid":true,"given":"Natalia","family":"Eberhardt","sequence":"first","affiliation":[{"name":"Department of Medicine, Leon H. 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