{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T03:38:58Z","timestamp":1768707538439,"version":"3.49.0"},"reference-count":59,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:sec>\n            <jats:title>Objective:<\/jats:title>\n            <jats:p>\n              Thrombus resolution is driven by leukocyte recruitment and thrombus angiogenesis. An effective inhibition of leukocyte transmigration in vitro is mediated by naturally occurring peptide B\u03b2\n              <jats:sub>15-42<\/jats:sub>\n              , which is a competitive inhibitor of the interaction between the N-terminus of the fibrin beta chain and vascular endothelial cadherin. We investigated the effect of B\u03b2\n              <jats:sub>15-42<\/jats:sub>\n              on thrombus resolution in a murine stagnant flow venous thrombosis model and studied B\u03b2\n              <jats:sub>15-42<\/jats:sub>\n              levels in venous thrombus of human patients.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Approach and Results:<\/jats:title>\n            <jats:p>\n              We investigated 2 mouse models of subtotal inferior vena cava ligation. In the first model, we ligated the inferior vena cava. In the second model, we additionally ligated all visible inferior vena cava side and back branches. Study groups of 8 to 12 weeks old BALB\/c mice were then injected intraperitoneal twice daily with 2.4 mg\/kg of B\u03b2\n              <jats:sub>15-42<\/jats:sub>\n              , unrelated control peptide or saline. B\u03b2\n              <jats:sub>15-42<\/jats:sub>\n              attenuated thrombus resolution after inferior vena cava ligation. We observed decreased numbers of thrombus macrophages and microvessels and less urokinase-type plasminogen activator expression in mice that were injected with B\u03b2\n              <jats:sub>15-42<\/jats:sub>\n              . Mechanistic experiments demonstrated that B\u03b2\n              <jats:sub>15-42<\/jats:sub>\n              blocks monocyte transmigration through an endothelial cell monolayer. Measurements of B\u03b2\n              <jats:sub>15-42<\/jats:sub>\n              in red clot and plasma of chronic thromboembolic pulmonary hypertension cases indicated high concentrations compared with controls.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>\n              Our data suggest that excess of the fibrin fragment B\u03b2\n              <jats:sub>15-42<\/jats:sub>\n              misguides thrombus resolution, presumably by inhibiting vascular endothelial cadherin-mediated leukocyte migration during early thrombus organization.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/atvbaha.121.316404","type":"journal-article","created":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T09:07:17Z","timestamp":1622711237000},"page":"2168-2180","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":6,"title":["Synthetic Fibrin-Derived B\u03b2\n            <sub>15-42<\/sub>\n            Peptide Delays Thrombus Resolution in a Mouse Model"],"prefix":"10.1161","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7235-2505","authenticated-orcid":true,"given":"Stella","family":"Chausheva","sequence":"first","affiliation":[{"name":"Division of Cardiology, Department of Internal Medicine II (S.C., S.S., I.M.L.), Medical University of Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bassam","family":"Redwan","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Klinik am Park, Klinikum Westfalen, Luenen, Germany (B.R.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Smriti","family":"Sharma","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Internal Medicine II (S.C., S.S., I.M.L.), Medical University of Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5254-6089","authenticated-orcid":true,"given":"Nara","family":"Marella","sequence":"additional","affiliation":[{"name":"CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Austria (N.M., A.C.M.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1202-5961","authenticated-orcid":true,"given":"Klaudia","family":"Schossleitner","sequence":"additional","affiliation":[{"name":"Skin and Endothelial Research Division, Department of Dermatology (K.S., P.P.), Medical University of Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0608-8176","authenticated-orcid":true,"given":"Andr\u00e9 C.","family":"Mueller","sequence":"additional","affiliation":[{"name":"CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Austria (N.M., A.C.M.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Petzelbauer","sequence":"additional","affiliation":[{"name":"Skin and Endothelial Research Division, Department of Dermatology (K.S., P.P.), Medical University of Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy","family":"Morris","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine, University of California San Diego (T.M.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0485-2692","authenticated-orcid":true,"given":"Irene M.","family":"Lang","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Internal Medicine II (S.C., S.S., I.M.L.), Medical University of Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.108.162289"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp048088"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.114.009309"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.015008"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200506-916OC"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2006.02.010"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-03-208264"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.182.6.855"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2013.09.024"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.90412.2008"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-006-0051-7"},{"key":"e_1_3_6_13_2","doi-asserted-by":"crossref","unstructured":"Bach TL Barsigian C Yaen CH Martinez J. 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