{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T01:15:28Z","timestamp":1776820528951,"version":"3.51.2"},"reference-count":47,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:sec>\n            <jats:title>Background:<\/jats:title>\n            <jats:p>Epicardial adipose tissue (EAT) contributes to coronary artery disease (CAD). EAT presents a specific lipidomic signature, showing increased ceramides and other proinflammatory lipids content. Besides, LPL (lipoprotein lipase) activity in EAT would contribute to its expansion, supplying fatty acids to the tissue. Our aim was to evaluate the relations between LPL activity, regulators of LPL, and ceramides in EAT from CAD patients.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>We studied patients undergoing coronary bypass graft (CAD, n=25) and patients without CAD (no CAD, n=14). EAT and subcutaneous AT (SAT) were obtained, tissue LPL activity and its regulator\u2019s expression (ANGPTL4, GPIHBP1 [glycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1], and PPAR\u03b3 [peroxisomal proliferator\u2013activated receptor \u03b3]) were assessed. Tissue lipidomes were evaluated by UHPLC-MS, in positive and negative ionization modes.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>\n              LPL activity was higher in EAT from CAD (\n              <jats:italic>P<\/jats:italic>\n              &lt;0.001), and in EAT than SAT in both groups (\n              <jats:italic>P<\/jats:italic>\n              &lt;0.001). ANGPTL4 levels were lower, GPIHBP1 and PPAR\u03b3 levels were higher in EAT from CAD (\n              <jats:italic>P<\/jats:italic>\n              &lt;0.001). In both groups, EAT exhibited more ceramide (\n              <jats:italic>P<\/jats:italic>\n              =0.01), directly associated with LPL activity, being the strongest association with Cer18:1\/24:1 (\n              <jats:italic>P<\/jats:italic>\n              &lt;0.001). EAT Cer18:1\/16:0 to Cer18:1\/24:0 and Cer18:1\/24:1 to 18:1\/24:0 ratios were higher in CAD (\n              <jats:italic>P<\/jats:italic>\n              =0.03;\n              <jats:italic>P<\/jats:italic>\n              &lt;0.001, respectively), the latter directly associated with LPL activity (\n              <jats:italic>r<\/jats:italic>\n              =0.63,\n              <jats:italic>P<\/jats:italic>\n              &lt;0.001) GPIHBP1 levels (\n              <jats:italic>r<\/jats:italic>\n              =0.68,\n              <jats:italic>P<\/jats:italic>\n              &lt;0.001), and inversely to EAT ANGPTL4 expression (\n              <jats:italic>r<\/jats:italic>\n              =\u22120.49,\n              <jats:italic>P<\/jats:italic>\n              =0.03). Pairwise partial correlation network showed associations among bioactive lipids and LPL and its regulators (\n              <jats:italic>P<\/jats:italic>\n              &lt;0.001 in all cases).\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>The association between LPL activity, total ceramide, and the atherogenic ceramide ratios highlights the importance of the enzyme and these bioactive lipids contributing to the different metabolic profile of EAT in CAD.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/atvbaha.122.317840","type":"journal-article","created":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T09:00:41Z","timestamp":1655370041000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":14,"title":["Epicardial Adipose Tissue Ceramides Are Related to Lipoprotein Lipase Activity in Coronary Artery Disease: Unfolding a Missing 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B.G.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f3nica","family":"Miksztowicz","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires, CONICET, Facultad de Farmacia y Bioqu\u00edmica, Argentina (M.B., V.M., G.B.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Doukbi","sequence":"additional","affiliation":[{"name":"Aix-Marseille University, INSERM, INRAE, C2VN, France (P.A., E.D., L.S., A.D., J.C.M., B.G.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ljubica","family":"Svilar","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires, Facultad de Farmacia y Bioqu\u00edmica, Instituto de Fisiopatolog\u00eda y Bioqu\u00edmica Cl\u00ednica (INFIBIOC), Departamento de Bioqu\u00edmica Cl\u00ednica, Laboratorio de L\u00edpidos y Aterosclerosis, Buenos Aires, Argentina (M.B., L.S., G.B.)."},{"name":"Aix-Marseille University, INSERM, INRAE, C2VN, France (P.A., E.D., L.S., A.D., J.C.M., B.G.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1970-0934","authenticated-orcid":true,"given":"Daniel","family":"Y\u00f1\u00f3n","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires, Hospital de Cl\u00ednicas \u201cJos\u00e9 de San Mart\u00edn\u201d, Divisi\u00f3n de Cirug\u00eda Cardiovascular, Argentina (D.Y., M.R.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Patricio","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Servicio de Docencia e Investigaci\u00f3n, Hospital Central de Formosa, Facultad de Ciencias de la Salud, Universidad Nacional de Formosa, Argentina (J.P.N.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Rubio","sequence":"additional","affiliation":[{"name":"Universidad de Buenos Aires, Hospital de Cl\u00ednicas \u201cJos\u00e9 de San Mart\u00edn\u201d, Divisi\u00f3n de Cirug\u00eda 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