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However, little is known about the heterogeneity of coronary artery ECs, and cellular identity responses to flow. Identifying endothelial subpopulations will contribute to the precise localization of vascular endothelial subpopulations, thus enabling the precision of vascular injury treatment.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>METHODS:<\/jats:title>\n            <jats:p>Here, we performed a single-cell RNA sequencing of 31\u2005962 cells and functional assays of 3 branches of the coronary arteries (right coronary artery\/circumflex left coronary artery\/anterior descending left coronary artery) in wild-type mice.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>RESULTS:<\/jats:title>\n            <jats:p>\n              We found a compendium of 7 distinct cell types in mouse coronary arteries, mainly ECs, granulocytes, cardiac myocytes, smooth muscle cells, lymphocytes, myeloid cells, and fibroblast cells, and showed spatial heterogeneity between arterial branches. Furthermore, we revealed a subpopulation of coronary artery ECs, CD133\n              <jats:sup>+<\/jats:sup>\n              TRPV4\n              <jats:sup>high<\/jats:sup>\n              ECs. TRPV4 (transient receptor potential vanilloid 4) in CD133\n              <jats:sup>+<\/jats:sup>\n              TRPV4\n              <jats:sup>high<\/jats:sup>\n              ECs is important for regulating vasodilation and coronary blood flow.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>CONCLUSIONS:<\/jats:title>\n            <jats:p>\n              Our study elucidates the nature and range of coronary arterial cell diversity and highlights the importance of coronary CD133\n              <jats:sup>+<\/jats:sup>\n              TRPV4\n              <jats:sup>high<\/jats:sup>\n              ECs in regulating coronary vascular tone.\n            <\/jats:p>\n          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